CA2194135C - Mono- et poly-antigenes recombines pour la detection de l'igm specifique du cytomegalovirus dans les serums humains par dosage immuno-enzymatique - Google Patents
Mono- et poly-antigenes recombines pour la detection de l'igm specifique du cytomegalovirus dans les serums humains par dosage immuno-enzymatique Download PDFInfo
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- CA2194135C CA2194135C CA002194135A CA2194135A CA2194135C CA 2194135 C CA2194135 C CA 2194135C CA 002194135 A CA002194135 A CA 002194135A CA 2194135 A CA2194135 A CA 2194135A CA 2194135 C CA2194135 C CA 2194135C
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- specific igm
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Mélange de matières protéiques de mono- et poly-épitopes pouvant entièrement remplacer les antigènes viraux lorsqu'il est utilisé dans un dosage immuno-enzymatique. Ce mélange comprend une protéine hybride de poly-épitope possédant une première région formée par une séquence d'acides aminés (H10) correspondant à celle des 233 derniers acides aminés de la terminaison COOH de la protéine virale p52 ou à une partie de celle-ci, une deuxième région formée par une séquence d'acides aminés (F3) correspondant à celle des 43 derniers acides aminés de la terminaison COOH de la protéine virale pp150 ou à une partie de celle-ci, et une troisième région formée par une séquence d'acides aminés (A1C2) correspondant à celle prise, dans le sens 5'}3', des acides aminés 595 à 614 de la même protéine virale pp150; et une association, d'une part, d'une deuxième protéine hybride comprenant une séquence d'acides aminés correspondant à celle prise, dans le sens 5'}3', des acides aminés 297 à 510 de la protéine virale de matrice majeure pp65 codée par le gène viral UL83, et d'autre part, une troisième protéine hybride comprenant une séquence d'acides aminés correspondant à celle prise, dans le sens 5'}3', des acides aminés 117 à 373 de la protéine virale d'assemblage pp38 codée par le gène viral UL80a. Ces trois protéines hybrides peuvent être associées les unes aux autres et utilisées dans la préparation d'une trousse d'essai de type ELISA pour le dépistage de l'IgM spécifique du cytomégalovirus dans les sérums humains.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITTO94A000543 | 1994-07-01 | ||
| IT94TO000543A IT1266740B1 (it) | 1994-07-01 | 1994-07-01 | Materiale proteico ricombinante legante anticorpi contro il citomegalovirus umano, reagenti diagnostici derivati da tale |
| PCT/IT1995/000073 WO1996001321A1 (fr) | 1994-07-01 | 1995-05-15 | Mono- et poly-antigenes recombines pour la detection de l'igm specifique du cytomegalovirus dans les serums humains par dosage immuno-enzymatique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2194135A1 CA2194135A1 (fr) | 1996-01-18 |
| CA2194135C true CA2194135C (fr) | 2006-07-18 |
Family
ID=36694025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002194135A Expired - Lifetime CA2194135C (fr) | 1994-07-01 | 1995-05-15 | Mono- et poly-antigenes recombines pour la detection de l'igm specifique du cytomegalovirus dans les serums humains par dosage immuno-enzymatique |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2194135C (fr) |
-
1995
- 1995-05-15 CA CA002194135A patent/CA2194135C/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2194135A1 (fr) | 1996-01-18 |
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| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20150515 |