CA2197117A1 - Agents therapeutiques antisens et triplex; methodes d'obtention - Google Patents
Agents therapeutiques antisens et triplex; methodes d'obtentionInfo
- Publication number
- CA2197117A1 CA2197117A1 CA002197117A CA2197117A CA2197117A1 CA 2197117 A1 CA2197117 A1 CA 2197117A1 CA 002197117 A CA002197117 A CA 002197117A CA 2197117 A CA2197117 A CA 2197117A CA 2197117 A1 CA2197117 A1 CA 2197117A1
- Authority
- CA
- Canada
- Prior art keywords
- intercalating
- compound
- strands
- derivatives
- attached
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 230000002829 reductive effect Effects 0.000 description 1
- 210000001533 respiratory mucosa Anatomy 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 108700004027 tat Genes Proteins 0.000 description 1
- 101150098170 tat gene Proteins 0.000 description 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 229940086542 triethylamine Drugs 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000001755 vocal effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3511—Conjugate intercalating or cleaving agent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Abstract
On décrit des oligonucléotides, antisens et anti-gène, et des oligonucleotides modifiés, auxquels sont rattachées plusieurs molécules d'intercalation, utiles dans des thérapies antisens et triplex. Du sang, du plasma sanguin ou des échantillons de sérum sont rendus nettement moins infectieux, lorsqu'ils sont infectés par des virus, tout en gardant leur intégrité aux fins d'analyse clinique. Des brins synthétiques d'oligonucléotides, et/ou des dérivés d'oligonucléotides résistant aux nucléases, se voient rattacher des molécules d'un agent d'intercalation et constituent un additif qu'on peut rajouter à un échantillon de sang devant subir des examens et procédures de type analytique. La stérilisation d'un nombre significatif de virus cibles intracellulaires présents dans l'échantillon rend celui-ci nettement moins susceptible d'infecter des personnes qui le manipulent. Un additif prenant la forme d'un élément de liaison approprié tel qu'un anticorps ou un aptamère, spécifique de certains épitopes ou sites de liaison présents sur des virus cibles libres ou extracellulaires, peut aussi convenir, seul ou combiné avec des ingrédients de brins cellulaires.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28859394A | 1994-08-10 | 1994-08-10 | |
| US08/288,593 | 1994-08-10 | ||
| US38949695A | 1995-02-16 | 1995-02-16 | |
| US08/389,496 | 1995-02-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2197117A1 true CA2197117A1 (fr) | 1996-02-22 |
Family
ID=26965112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002197117A Abandoned CA2197117A1 (fr) | 1994-08-10 | 1995-08-09 | Agents therapeutiques antisens et triplex; methodes d'obtention |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0774902A1 (fr) |
| JP (1) | JPH10504819A (fr) |
| CA (1) | CA2197117A1 (fr) |
| WO (1) | WO1996004788A1 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6776986B1 (en) | 1996-06-06 | 2004-08-17 | Novartis Ag | Inhibition of HIV-1 replication by antisense RNA expression |
| AU2006251496B2 (en) | 2005-05-25 | 2012-11-22 | Tina Holding Aps | Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (TINA) and process for the preparation of TINA |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2540122B1 (fr) * | 1983-01-27 | 1985-11-29 | Centre Nat Rech Scient | Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application |
| US4806463A (en) * | 1986-05-23 | 1989-02-21 | Worcester Foundation For Experimental Biology | Inhibition of HTLV-III by exogenous oligonucleotides |
-
1995
- 1995-08-09 WO PCT/US1995/010055 patent/WO1996004788A1/fr not_active Ceased
- 1995-08-09 EP EP95930802A patent/EP0774902A1/fr not_active Withdrawn
- 1995-08-09 CA CA002197117A patent/CA2197117A1/fr not_active Abandoned
- 1995-08-09 JP JP8507452A patent/JPH10504819A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MX9700991A (es) | 1998-05-31 |
| JPH10504819A (ja) | 1998-05-12 |
| EP0774902A1 (fr) | 1997-05-28 |
| WO1996004788A1 (fr) | 1996-02-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |