CA2199810A1 - Cultures tridimensionnelles de cellules humaines sur des structures de valvules cardiaques, et leurs utilisations - Google Patents

Cultures tridimensionnelles de cellules humaines sur des structures de valvules cardiaques, et leurs utilisations

Info

Publication number
CA2199810A1
CA2199810A1 CA002199810A CA2199810A CA2199810A1 CA 2199810 A1 CA2199810 A1 CA 2199810A1 CA 002199810 A CA002199810 A CA 002199810A CA 2199810 A CA2199810 A CA 2199810A CA 2199810 A1 CA2199810 A1 CA 2199810A1
Authority
CA
Canada
Prior art keywords
cells
heart valve
stromal
fibroblasts
stromal cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002199810A
Other languages
English (en)
Inventor
Gail K. Naughton
Brian A. Naughton
Anthony F. Purchio
Lee K. Landeen
Joan Zeltinger
Todd D. Campbell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Tissue Sciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2199810A1 publication Critical patent/CA2199810A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
    • A61F2/06—Blood vessels
    • A61F2/062—Apparatus for the production of blood vessels made from natural tissue or with layers of living cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/24—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body
    • A61F2/2412—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body with soft flexible valve members, e.g. tissue valves shaped like natural valves
    • A61F2/2415—Manufacturing methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3625—Vascular tissue, e.g. heart valves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Vascular Medicine (AREA)
  • Zoology (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Manufacturing & Machinery (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Prostheses (AREA)

Abstract

L'invention concerne un procédé qui permet de faire croître différents tissus et cellules dans des cultures tridimensionnelles in vitro en utilisant des fibroblastes humains dans des milieux de culture. Des cellules du stroma, comprenant notamment des fibroblastes dermiques et cardiaques humains, sont inoculés et croissent sur une grille ou structure tridimensionnellle. Ces fibroblastes humains secrètent des protéines de matrice humaine destinées aà compléter et remplacer la matrice porcine existante composée de valvules cardiaques ou de parois et valves aortiques privées de leurs cellules et prenant la forme de reconstructions tridimensionnelles dotées d'espaces intersticiels reliés par les cellules du stroma. Le tissu du stroma vivant ainsi formé fournit un support ainsi que des facteurs de croissance et de régulation nécessaires pour assurer la viabilité et la prolifération à long terme des cellules mises en culture et/ou implantées in vivo. Lorsqu'elles croissent sur cette structure tridimensionnelle, les cellules qui prolifèrent deviennent matures et se différencient nettement pour former des composants de tissus analogues à leur équivalents in vivo.
CA002199810A 1994-09-12 1995-09-08 Cultures tridimensionnelles de cellules humaines sur des structures de valvules cardiaques, et leurs utilisations Abandoned CA2199810A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30406294A 1994-09-12 1994-09-12
US304,062 1994-09-12
US48816595A 1995-06-07 1995-06-07
US488,165 1995-06-07

Publications (1)

Publication Number Publication Date
CA2199810A1 true CA2199810A1 (fr) 1996-03-21

Family

ID=26973791

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002199810A Abandoned CA2199810A1 (fr) 1994-09-12 1995-09-08 Cultures tridimensionnelles de cellules humaines sur des structures de valvules cardiaques, et leurs utilisations

Country Status (8)

Country Link
EP (1) EP0781116A4 (fr)
JP (1) JPH10511563A (fr)
KR (1) KR970705951A (fr)
AU (1) AU700911B2 (fr)
CA (1) CA2199810A1 (fr)
IL (1) IL115261A0 (fr)
NZ (1) NZ293419A (fr)
WO (1) WO1996008213A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9643153B2 (en) 2014-05-19 2017-05-09 Evonik Degussa Gmbh Membrane-supported catalyst removal in the epoxidation of cyclic unsaturated C12 compounds, for example cyclododecene (CDEN)

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US6284284B1 (en) 1995-06-06 2001-09-04 Advanced Tissue Sciences, Inc. Compositions and methods for production and use of an injectable naturally secreted extracellular matrix
US6121041A (en) * 1996-07-31 2000-09-19 St. Jude Medical, Inc. Use of microorganisms for decellularizing bioprosthetic tissue
US6206917B1 (en) * 1997-05-02 2001-03-27 St. Jude Medical, Inc. Differential treatment of prosthetic devices
ES2175753T3 (es) * 1997-06-27 2002-11-16 Augustinus Bader Injerto biosintetico y metodo para su produccion.
AU747166B2 (en) 1997-10-31 2002-05-09 Children's Medical Center Corporation Bladder reconstruction
EP1782849A3 (fr) * 1999-04-12 2007-05-23 Theregen, Inc. Tissu stromal tridimensionnel
US20030007954A1 (en) * 1999-04-12 2003-01-09 Gail K. Naughton Methods for using a three-dimensional stromal tissue to promote angiogenesis
EP1196206A1 (fr) * 1999-07-09 2002-04-17 Advanced Tissue Sciences, Inc. Appareil revetu d'une matrice extracellulaire naturellement secretee par l'homme
JP3603179B2 (ja) 1999-09-09 2004-12-22 グンゼ株式会社 心血管系組織培養用基材および組織再生法
JP2001120582A (ja) 1999-10-22 2001-05-08 Gunze Ltd 人工心臓弁およびその作製方法
US6432712B1 (en) * 1999-11-22 2002-08-13 Bioscience Consultants, Llc Transplantable recellularized and reendothelialized vascular tissue graft
US6652583B2 (en) 2000-04-07 2003-11-25 Rhode Island Hospital Cardiac valve replacement
DE10026482A1 (de) * 2000-05-29 2001-12-13 Augustinus Bader Verfahren zur Herstellung eines bioartifiziellen Transplantats
DE10026480A1 (de) 2000-05-29 2001-12-13 Augustinus Bader Verfahren zur Herstellung eines empfängerspezifischen Gewebe-Transplantats oder -Implantats
DE10058240A1 (de) * 2000-11-17 2002-05-29 Auto Tissue Gmbh Verfahren und Vorrichtung zur Herstellung biologischer Prothesen
EP2292278B1 (fr) * 2001-11-16 2013-04-24 Children's Medical Center Corporation Augmentation de la fonction d'un organe
WO2005023321A2 (fr) 2003-09-04 2005-03-17 Cook Biotech Incorporated Materiaux composites de matrice extracellulaire, leur fabrication et utilisation
DE10350287A1 (de) 2003-10-24 2005-05-25 Deutsche Institute für Textil- und Faserforschung Stuttgart - Stiftung des öffentlichen Rechts Kardiovaskuläres Implantat, Verfahren und Vorrichtung zur Herstellung und Bereitstellung für die Chirurgie
EP1863545B1 (fr) * 2005-03-19 2015-11-18 Cook Biotech, Inc. Implants prothetiques comprenant un materiau composite a matrice extracellulaire
MX369594B (es) 2005-08-26 2019-11-13 Univ Minnesota Descelularizacion y recelularizacion de organos y tejidos.
US20080176206A1 (en) 2007-01-18 2008-07-24 Toshiharu Shinoka Cardiovascular tissue culture substrate
US9580688B2 (en) 2007-06-08 2017-02-28 Wake Forest University Health Sciences Kidney structures and methods of forming the same
EP2162529B1 (fr) 2007-06-08 2019-03-27 Wake Forest University Health Sciences Thérapie cellulaire sélective pour le traitement de l'insuffisance rénale
EP2085054A1 (fr) * 2008-02-01 2009-08-05 Technische Universiteit Eindhoven Procédé de fabrication de construction créée par tissu
WO2010057013A1 (fr) 2008-11-14 2010-05-20 Wake Forest University Health Sciences Therapie cellulaire selective pour le traitement d'insuffisance renale
AU2010233169A1 (en) 2009-04-09 2011-11-03 Arizona Board Of Regents On Behalf Of The University Of Arizona Cellular seeding and co-culture of a three dimensional fibroblast construct
RU2611361C2 (ru) 2010-09-01 2017-02-21 Риджентс Оф Дзе Юниверсити Оф Миннесота Способы рецеллюляризации ткани или органа для улучшения приживления трансплантата
US9968446B2 (en) 2011-03-23 2018-05-15 The Regents Of The University Of California Tubular scaffold for fabrication of heart valves
US10016461B2 (en) 2012-12-03 2018-07-10 The Regents Of The University Of California Apparatus and process for growing a heart valve in three-dimensions
EP2841010B1 (fr) 2012-04-24 2023-08-23 Harvard Apparatus Regenerative Technology, Inc. Supports pour échafaudages tissulaires modifiés
US9290738B2 (en) 2012-06-13 2016-03-22 Miromatrix Medical Inc. Methods of decellularizing bone
JP2016513465A (ja) 2013-03-15 2016-05-16 ミロマトリックス メディカル インコーポレイティド 膵島細胞の再細胞化のための灌流型脱細胞化肝臓の使用
DE102014209413A1 (de) 2014-05-19 2015-11-19 Evonik Degussa Gmbh Membrangestützte Katalysatorabtrennung bei der Epoxidierung von Fettsäurealkylestern
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EP4052734B1 (fr) * 2021-03-02 2024-04-24 Solidified Inc. Échafaudage de tissus immunocompatible et ses procédés de formation
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9643153B2 (en) 2014-05-19 2017-05-09 Evonik Degussa Gmbh Membrane-supported catalyst removal in the epoxidation of cyclic unsaturated C12 compounds, for example cyclododecene (CDEN)

Also Published As

Publication number Publication date
JPH10511563A (ja) 1998-11-10
AU3585595A (en) 1996-03-29
KR970705951A (ko) 1997-11-03
IL115261A0 (en) 1995-12-31
NZ293419A (en) 1998-11-25
EP0781116A4 (fr) 1999-08-25
EP0781116A1 (fr) 1997-07-02
WO1996008213A1 (fr) 1996-03-21
AU700911B2 (en) 1999-01-14

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued
FZDE Discontinued

Effective date: 20030908