CA2206265C - Procede de reduction de la concentration d'arn dans un melange de matieres biologiques a l'aide de diatomite - Google Patents

Procede de reduction de la concentration d'arn dans un melange de matieres biologiques a l'aide de diatomite Download PDF

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Publication number
CA2206265C
CA2206265C CA002206265A CA2206265A CA2206265C CA 2206265 C CA2206265 C CA 2206265C CA 002206265 A CA002206265 A CA 002206265A CA 2206265 A CA2206265 A CA 2206265A CA 2206265 C CA2206265 C CA 2206265C
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CA
Canada
Prior art keywords
rna
concentration
diatomaceous earth
dna
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002206265A
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English (en)
Other versions
CA2206265A1 (fr
Inventor
Nancy Horn
Magda Marquet
Jennifer Meek
Gregg Budahazi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/372,236 external-priority patent/US5576196A/en
Application filed by Vical Inc filed Critical Vical Inc
Publication of CA2206265A1 publication Critical patent/CA2206265A1/fr
Application granted granted Critical
Publication of CA2206265C publication Critical patent/CA2206265C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention se rapporte à un procédé de réduction de la concentration d'ARN dans un mélange de matières biologiques, ce procédé consistant à: (a) obtenir un mélange de matières biologiques possédant une première concentration d'ARN; (b) filtrer le mélange en le faisant passer dans une diatomite pour obtenir un filtrat possédant une seconde concentration d'ARN, cette seconde concentration étant inférieure à la première; et (c) récupérer le filtrat dont la concentration d'ARN a été réduite. Selon un aspect de l'invention, la diatomite sert à purifier l'ADN plasmidique de recombinaison à partir d'un composant d'ARN dans un mélange de matières biologiques, et selon un autre aspect, à séparer différentes formes d'ARN soluble dans un mélange de matières biologiques.
CA002206265A 1995-01-13 1996-01-03 Procede de reduction de la concentration d'arn dans un melange de matieres biologiques a l'aide de diatomite Expired - Fee Related CA2206265C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/372,236 US5576196A (en) 1995-01-13 1995-01-13 Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US08/372,236 1995-01-13
PCT/US1996/000247 WO1996021729A1 (fr) 1995-01-13 1996-01-03 Procede de reduction de la concentration d'arn dans un melange de matieres biologiques a l'aide de diatomite

Publications (2)

Publication Number Publication Date
CA2206265A1 CA2206265A1 (fr) 1996-07-18
CA2206265C true CA2206265C (fr) 2007-05-29

Family

ID=38147157

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002206265A Expired - Fee Related CA2206265C (fr) 1995-01-13 1996-01-03 Procede de reduction de la concentration d'arn dans un melange de matieres biologiques a l'aide de diatomite

Country Status (1)

Country Link
CA (1) CA2206265C (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025252480A1 (fr) * 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Procédé de purification d'adn plasmidique

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112619596A (zh) * 2020-12-01 2021-04-09 上海江夏血液技术有限公司 吸附膜及其制备方法和复合膜组件及过滤器

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025252480A1 (fr) * 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Procédé de purification d'adn plasmidique

Also Published As

Publication number Publication date
CA2206265A1 (fr) 1996-07-18

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Effective date: 20150105