CA2206984A1 - Manipulation genetique de thioesterases vegetales et thioesterases vegetales presentant une nouvelle specificite de substrat - Google Patents

Manipulation genetique de thioesterases vegetales et thioesterases vegetales presentant une nouvelle specificite de substrat

Info

Publication number
CA2206984A1
CA2206984A1 CA 2206984 CA2206984A CA2206984A1 CA 2206984 A1 CA2206984 A1 CA 2206984A1 CA 2206984 CA2206984 CA 2206984 CA 2206984 A CA2206984 A CA 2206984A CA 2206984 A1 CA2206984 A1 CA 2206984A1
Authority
CA
Canada
Prior art keywords
thioesterase
acp
plant
acyl
thioesterases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2206984
Other languages
English (en)
Inventor
Ling Yuan
Vic C. Knauf
Jean Kridl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/440,845 external-priority patent/US5955329A/en
Application filed by Individual filed Critical Individual
Publication of CA2206984A1 publication Critical patent/CA2206984A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés pour modifier la spécificité de substrats de thioestérases acyle-ACP de plantes, et des thioestérases acyle-ACP végétales obtenues par génie génétique. Les deux tiers de la partie terminale C des thioestérases végétales sont identifiées comme devant subir ces modifications. L'invention traite également de séquences d'ADN et de produits de recombinaison pour l'expression de thioestérases obtenues par génie génétique, ainsi que les nouvelles thioestérases ainsi obtenues. Ces séquences d'ADN peuvent être utilisées pour l'expression des thioestérases obtenues par génie génétique dans des cellules hôtes, en particulier, dans les cellules de graines de plantes à graines oléagineuses, pour modifier la composition des acides gras. Il est possible de modifier une thioestérase acyle-ACP préférant un substrat C12 telle que décrite ici pour obtenir une thioestérase végétale présentant approximativement des activités semblables sur les substrats C14 et C12. D'autres modifications de l'enzyme C12 permettent de produire une thioestérase présentant une activité supérieure sur un substrat C14 par rapport aux substrats C12.
CA 2206984 1995-05-15 1996-05-15 Manipulation genetique de thioesterases vegetales et thioesterases vegetales presentant une nouvelle specificite de substrat Abandoned CA2206984A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/440,845 US5955329A (en) 1995-05-15 1995-05-15 Engineering plant thioesterases for altered substrate specificity
US53708395A 1995-09-29 1995-09-29
US08/440,845 1995-09-29
US08/537,083 1995-09-29

Publications (1)

Publication Number Publication Date
CA2206984A1 true CA2206984A1 (fr) 1996-11-21

Family

ID=27032574

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2206984 Abandoned CA2206984A1 (fr) 1995-05-15 1996-05-15 Manipulation genetique de thioesterases vegetales et thioesterases vegetales presentant une nouvelle specificite de substrat

Country Status (4)

Country Link
EP (1) EP0830453A2 (fr)
JP (1) JPH11505115A (fr)
CA (1) CA2206984A1 (fr)
WO (1) WO1996036719A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6150512A (en) * 1995-05-15 2000-11-21 Yuan; Ling Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity
WO1997012047A1 (fr) * 1995-09-29 1997-04-03 Calgene, Inc. Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales
US6331664B1 (en) 1997-05-05 2001-12-18 Dow Agrosciences Llc Acyl-ACP thioesterase nucleic acids from maize and methods of altering palmitic acid levels in transgenic plants therewith
US6365802B2 (en) 1998-08-14 2002-04-02 Calgene Llc Methods for increasing stearate content in soybean oil
WO2000075343A2 (fr) * 1999-06-09 2000-12-14 Calgene Llc MISE AU POINT DE LA SYNTHASE DE β-CETOACYL ACP AYANT UNE NOUVELLE SPECIFICITE DE SUBSTRAT
US6770465B1 (en) 1999-06-09 2004-08-03 Calgene Llc Engineering B-ketoacyl ACP synthase for novel substrate specificity
CA2782423C (fr) 2009-12-18 2019-06-18 Cargill Incorporated Plantes du genre brassica donnant des huiles a faible teneur totale en acides gras satures
JP5798729B2 (ja) 2009-12-25 2015-10-21 花王株式会社 チオエステラーゼ改変体を用いた脂肪酸含有脂質の製造方法
AU2013367325B2 (en) 2012-12-27 2019-12-19 Kao Corporation Acyl-ACP thioesterase
US9567615B2 (en) * 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9828613B2 (en) 2013-07-12 2017-11-28 Kao Corporation Acyl-ACP thioesterase
US10550412B2 (en) 2014-06-20 2020-02-04 Kao Corporation Method of producing lipid
EP3660149A1 (fr) 2014-07-24 2020-06-03 Corbion Biotech, Inc. Variantes de thioestérases et procédés d'utilisation
CN107208103A (zh) 2014-09-18 2017-09-26 泰拉瑞亚控股公司 酰基‑acp硫酯酶及其突变体
CN111094558A (zh) * 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
WO2019203073A1 (fr) * 2018-04-19 2019-10-24 花王株式会社 Procédé de production de lipides
US20210180094A1 (en) * 2018-04-19 2021-06-17 Kao Corporation Method of Producing Lipid
CN120026040B (zh) * 2025-03-21 2025-11-21 吉林大学 玉米单酰基甘油酯酶基因ZmMAGLL及应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases

Also Published As

Publication number Publication date
WO1996036719A1 (fr) 1996-11-21
EP0830453A2 (fr) 1998-03-25
JPH11505115A (ja) 1999-05-18

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Legal Events

Date Code Title Description
FZDE Dead