CA2210012C - Composition non corrosive et methodes utiles pour l'extraction d'acides nucleiques - Google Patents

Composition non corrosive et methodes utiles pour l'extraction d'acides nucleiques Download PDF

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Publication number: CA2210012C
Authority: CA; Canada
Prior art keywords: extraction; nucleic acid; sample; nucleic acids; aqueous phase
Prior art date: 1990-07-13
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002210012A

Other languages

English (en)

Other versions

CA2210012A1 (fr

Inventor

Jeffrey Van Ness

B. Melina Cimler

Rich B. Meyer, Jr.

Nicholaas Marthinus Johannes Vermuelen

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Becton Dickinson and Co

Original Assignee

Becton Dickinson and Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-07-13

Filing date

1991-07-12

Publication date

2001-07-10

1991-02-01 Priority claimed from US07/649,389 external-priority patent/US5130423A/en

1991-07-12 Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co

1991-07-12 Priority claimed from CA002087105A external-priority patent/CA2087105C/fr

1992-01-14 Publication of CA2210012A1 publication Critical patent/CA2210012A1/fr

2001-07-10 Application granted granted Critical

2001-07-10 Publication of CA2210012C publication Critical patent/CA2210012C/fr

2011-07-12 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

150000007523 nucleic acids Chemical class 0.000 title claims abstract description 117
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239000000203 mixture Substances 0.000 title claims abstract description 29
238000000605 extraction Methods 0.000 title abstract description 101
230000009972 noncorrosive effect Effects 0.000 title description 3
WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 claims abstract description 71
239000008346 aqueous phase Substances 0.000 claims abstract description 38
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238000005192 partition Methods 0.000 claims description 6
GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 claims description 4
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WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 claims description 2
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229910000271 hectorite Inorganic materials 0.000 description 1
238000000265 homogenisation Methods 0.000 description 1
ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 1
238000003018 immunoassay Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
150000002540 isothiocyanates Chemical class 0.000 description 1
239000007788 liquid Substances 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
238000000464 low-speed centrifugation Methods 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
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235000010335 lysozyme Nutrition 0.000 description 1
238000002844 melting Methods 0.000 description 1
230000008018 melting Effects 0.000 description 1
239000011325 microbead Substances 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
229910052901 montmorillonite Inorganic materials 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
238000007899 nucleic acid hybridization Methods 0.000 description 1
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125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
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239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
210000001938 protoplast Anatomy 0.000 description 1
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210000001995 reticulocyte Anatomy 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
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229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
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NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
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230000002194 synthesizing effect Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000011592 zinc chloride Substances 0.000 description 1
235000005074 zinc chloride Nutrition 0.000 description 1
JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1

Landscapes

Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

CA002210012A 1990-07-13 1991-07-12 Composition non corrosive et methodes utiles pour l'extraction d'acides nucleiques Expired - Lifetime CA2210012C (fr)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US55274590A	1990-07-13	1990-07-13
US552,745		1990-07-13
US07/649,389 US5130423A (en)	1990-07-13	1991-02-01	Non-corrosive compositions and methods useful for the extraction of nucleic acids
US649,389		1991-02-01
CA002087105A CA2087105C (fr)	1990-07-13	1991-07-12	Composition non corrosive et methodes utilisees pour extraire les acides nucleiques

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CA2210012C true CA2210012C (fr)	2001-07-10

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Publication	Publication Date	Title
US5130423A (en)	1992-07-14	Non-corrosive compositions and methods useful for the extraction of nucleic acids
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US20090130687A1 (en)	2009-05-21	Formulations and method isolating nucleic acids from arbitrary complex starting materials and subsequent complex genetic materials
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