CA2214800C - Chambre d'electroporation en flux et procede associe - Google Patents

Chambre d'electroporation en flux et procede associe Download PDF

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Publication number
CA2214800C
CA2214800C CA002214800A CA2214800A CA2214800C CA 2214800 C CA2214800 C CA 2214800C CA 002214800 A CA002214800 A CA 002214800A CA 2214800 A CA2214800 A CA 2214800A CA 2214800 C CA2214800 C CA 2214800C
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CA
Canada
Prior art keywords
electrodes
biological particles
red blood
cell
flow path
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002214800A
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English (en)
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CA2214800A1 (fr
Inventor
Peter M. Meserol
Rita C. Prodell
Jesse L. Acker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxcyte Inc
Original Assignee
THERAMED Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/402,145 external-priority patent/US5720921A/en
Application filed by THERAMED Inc filed Critical THERAMED Inc
Priority to CA002363548A priority Critical patent/CA2363548A1/fr
Publication of CA2214800A1 publication Critical patent/CA2214800A1/fr
Application granted granted Critical
Publication of CA2214800C publication Critical patent/CA2214800C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3687—Chemical treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3621—Extra-corporeal blood circuits
    • A61M1/3623—Means for actively controlling temperature of blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3692—Washing or rinsing blood or blood constituents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • G—PHYSICS
    • G05—CONTROLLING; REGULATING
    • G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
    • G05D23/00—Control of temperature
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3616—Batch-type treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0429—Red blood cells; Erythrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/33—Controlling, regulating or measuring
    • A61M2205/3306—Optical measuring means
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/33—Controlling, regulating or measuring
    • A61M2205/3379—Masses, volumes, levels of fluids in reservoirs, flow rates
    • A61M2205/3382—Upper level detectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/36—General characteristics of the apparatus related to heating or cooling
    • A61M2205/3606—General characteristics of the apparatus related to heating or cooling cooled
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2205/00—General characteristics of the apparatus
    • A61M2205/36—General characteristics of the apparatus related to heating or cooling
    • A61M2205/3673—General characteristics of the apparatus related to heating or cooling thermo-electric, e.g. Peltier effect, thermocouples, semi-conductors

Landscapes

  • Health & Medical Sciences (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Cardiology (AREA)
  • Public Health (AREA)
  • Anesthesiology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Electromagnetism (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Automation & Control Theory (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • External Artificial Organs (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Glass Compositions (AREA)
  • Physical Deposition Of Substances That Are Components Of Semiconductor Devices (AREA)

Abstract

Cette invention se rapporte à un procédé et à un appareil qui permettent d'encapsuler des substances biologiquement actives dans des globules rouges du sang et qui se caractérisent par un système d'électroporation autonome à flux continu éventuellement automatisé (5), qui permet de prélever du sang d'un patient, de séparer les globules rouges et, éventuellement, de recombiner le plasma et les globules rouges modifiés, afin de produire du sang ayant des caractéristiques biologiques modifiées. Cette invention est particulièrement adaptée pour encapsuler des effecteurs allostériques d'hémoglobine, afin de réduire l'affinité des hérythrocytes pour l'oxygène et afin d'améliorer la libération de l'oxygène contenue dans les hérythrocytes de tissus. Le sang est introduit dans le système (5) par une entrée (11) et mélangé à un anticoagulant (27). Le mélange de sang et d'anticoagulant est ensuite amené à passer à travers un filtre (18), jusqu'à atteindre un bol (44) de séparation et de lavage du sang. Le plasma et les globules blancs sont introduits dans le réservoir de plasma (89), tandis que les globules rouges sont retenus dans le bol de lavage (44). Les globules rouges séparés sont ensuite extraits par pompage et mélangés au niveau d'une jonction (67) avec une solution d'hexaphosphate d'inositol (HPI) provenant d'un réservoir (50). Ce mélange est refroidi par un serpentin de refroidissement (68) et ensuite pompé dans une chambre d'électroporation (72) pour y être traité.
CA002214800A 1995-03-10 1996-03-11 Chambre d'electroporation en flux et procede associe Expired - Lifetime CA2214800C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002363548A CA2363548A1 (fr) 1995-03-10 1996-03-11 Chambre d'electroporation en flux et procede associe

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/402,145 US5720921A (en) 1995-03-10 1995-03-10 Flow electroporation chamber and method
US490695P 1995-10-06 1995-10-06
US60/004,906 1995-10-06
US08/402,145 1995-10-06
PCT/US1996/003211 WO1996028199A1 (fr) 1995-03-10 1996-03-11 Chambre d'electroporation en flux et procede associe

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002363548A Division CA2363548A1 (fr) 1995-03-10 1996-03-11 Chambre d'electroporation en flux et procede associe

Publications (2)

Publication Number Publication Date
CA2214800A1 CA2214800A1 (fr) 1996-09-19
CA2214800C true CA2214800C (fr) 2002-01-22

Family

ID=26673634

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002214800A Expired - Lifetime CA2214800C (fr) 1995-03-10 1996-03-11 Chambre d'electroporation en flux et procede associe

Country Status (6)

Country Link
EP (1) EP0814855A4 (fr)
JP (2) JP3859709B2 (fr)
KR (1) KR19980702848A (fr)
CN (1) CN1195997A (fr)
AU (1) AU5305096A (fr)
CA (1) CA2214800C (fr)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
EP1161270B1 (fr) * 1999-03-17 2007-01-10 Haemonetics Corporation Systeme de traitement du sang
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
AU2002326717A1 (en) 2001-08-22 2003-03-10 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7601268B2 (en) * 2003-05-27 2009-10-13 Haemonetics Corporation Continuous blood filtration and method of use
US7771984B2 (en) 2004-05-12 2010-08-10 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
JP4519915B2 (ja) * 2004-06-12 2010-08-04 インビトロゲン シンガポール ピーティーイー．リミテッド． 中空構造を有する電気穿孔装置
CN1965079B (zh) * 2004-06-12 2012-12-05 英杰生命新加坡私人有限公司 具有长中空件的电穿孔装置
KR101084528B1 (ko) * 2008-04-15 2011-11-18 인비트로겐 싱가포르 피티이. 엘티디. 전기천공 장치용 파이펫 팁
CN101857836B (zh) * 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 一种流式电穿孔装置及系统
EP2338356A1 (fr) * 2009-12-23 2011-06-29 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Système de réacteur pour électroporation
GB201012521D0 (en) * 2010-07-27 2010-09-08 Univ Strathclyde Integrated perfusion system
CN101928666B (zh) * 2010-07-30 2013-04-03 北京大学 一种流式电穿孔装置及系统
EP3673845B1 (fr) * 2013-03-13 2024-04-17 Nuvaira, Inc. Cartouche d'echangeur de chaleur à usage unique
JP6683598B2 (ja) * 2013-03-15 2020-04-22 マックスサイト インコーポレーティッド 安定なトランスフェクトされた細胞を生成するための方法および組成物
ES2807439T3 (es) * 2015-03-31 2021-02-23 Oncosec Medical Inc Sistemas para electroporación mejorada basada en la detección de tejidos
CN107980059B (zh) 2015-04-13 2022-11-11 美克斯细胞有限公司 用于修饰基因组dna的方法和组合物
WO2016183032A1 (fr) * 2015-05-08 2016-11-17 Biomagnetic Solutions Llc Appareil et procédé de séparation de cellules immunomagnétique
KR102516995B1 (ko) * 2016-04-05 2023-03-31 제네럴 일렉트릭 컴퍼니 조정가능한 성장 인자 레벨을 가진 활성화된 혈소판 조성
WO2018211500A1 (fr) * 2017-05-14 2018-11-22 Paragate Medical Ltd. Système implantable d'extraction de fluide
JP6985508B2 (ja) * 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
CN110527625A (zh) * 2018-05-24 2019-12-03 苏州壹达生物科技有限公司 一种结合高压和低压模式的流式电转染装置
CN108795750A (zh) * 2018-05-28 2018-11-13 深圳先进技术研究院 一种带冷却功能的电穿孔装置
AU2019331113A1 (en) * 2018-08-31 2021-03-18 The Charles Stark Draper Laboratory, Inc. Method and apparatus for high throughput high efficiency transfection of cells
WO2020130106A1 (fr) * 2018-12-20 2020-06-25 国立大学法人豊橋技術科学大学 Dispositif d'électroporation et procédé de production de cellules avec une substance étrangère introduite
US11071860B2 (en) 2019-02-06 2021-07-27 Oncosec Medical Incorporated Systems and methods for detecting fault conditions in electroporation therapy
JP7248721B2 (ja) * 2021-02-17 2023-03-29 ゼネラル・エレクトリック・カンパニイ 成長因子レベルを調節可能な活性化血小板組成物
EP4310173A1 (fr) 2021-04-22 2024-01-24 FUJIFILM Corporation Dispositif de perforation électrique, procédé d'introduction de matériau bioactif, procédé de production de biomatériau issu de tissu, et biomatériau issu de tissu
JPWO2023157673A1 (fr) 2022-02-21 2023-08-24
JPWO2023223931A1 (fr) 2022-05-16 2023-11-23
CN116077753B (zh) * 2023-01-13 2026-03-31 海尔血液技术重庆有限公司 血浆分离机
WO2024204415A1 (fr) * 2023-03-27 2024-10-03 富士フイルム株式会社 Procédé de production virale
KR20260006618A (ko) 2023-06-01 2026-01-13 후지필름 가부시키가이샤 일렉트로포레이션 방법, 유용 물질의 제조 방법, 유로 디바이스 및 일렉트로포레이션 장치
WO2026070754A1 (fr) * 2024-09-27 2026-04-02 富士フイルム株式会社 Procédé de nettoyage pour dispositif à canal d'écoulement

Also Published As

Publication number Publication date
AU5305096A (en) 1996-10-02
JP2007007430A (ja) 2007-01-18
CA2214800A1 (fr) 1996-09-19
KR19980702848A (ko) 1998-08-05
JP4171755B2 (ja) 2008-10-29
JP3859709B2 (ja) 2006-12-20
EP0814855A1 (fr) 1998-01-07
CN1195997A (zh) 1998-10-14
EP0814855A4 (fr) 2002-07-17
JPH11502133A (ja) 1999-02-23

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