CA2221192C - Embedded expression of heterologous proteins - Google Patents

Embedded expression of heterologous proteins Download PDF

Info

Publication number: CA2221192C
Authority: CA; Canada
Prior art keywords: protein; cks; heterologous; dna; domain
Prior art date: 1995-05-15
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA2221192A

Other languages

English (en)

French (fr)

Other versions

CA2221192A1 (en

Inventor

Gregory T. Maine

Linda E. Chovan

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abbott Laboratories

Original Assignee

Abbott Laboratories

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-05-15

Filing date

1996-05-14

Publication date

2011-11-01

1996-05-14 Application filed by Abbott Laboratories filed Critical Abbott Laboratories

1996-11-21 Publication of CA2221192A1 publication Critical patent/CA2221192A1/en

2011-11-01 Application granted granted Critical

2011-11-01 Publication of CA2221192C publication Critical patent/CA2221192C/en

2016-05-14 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Virology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
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CA2221192A 1995-05-15 1996-05-14 Embedded expression of heterologous proteins Expired - Fee Related CA2221192C (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US44114195A	1995-05-15	1995-05-15
US08/441,141		1995-05-15
PCT/US1996/006841 WO1996036722A2 (en)	1995-05-15	1996-05-14	Embedded expression of heterologous proteins

Publications (2)

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CA2221192A1 CA2221192A1 (en)	1996-11-21
CA2221192C true CA2221192C (en)	2011-11-01

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CA2221192A Expired - Fee Related CA2221192C (en)	1995-05-15	1996-05-14	Embedded expression of heterologous proteins

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EP (1)	EP0826060B1 (de)
JP (1)	JP4108122B2 (de)
AT (1)	ATE406451T1 (de)
CA (1)	CA2221192C (de)
DE (1)	DE69637659D1 (de)
ES (1)	ES2313727T3 (de)
WO (1)	WO1996036722A2 (de)

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* Cited by examiner, † Cited by third party
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IT1290804B1 (it)	1996-08-16	1998-12-11	Abbott Lab	Saggio western blot perfezionato per l'individuazione di anticorpi specifici contro il virus hcmv

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* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5124255A (en) *	1988-03-11	1992-06-23	Abbott Laboratories	CKS method of protein synthesis
DE3828666A1 (de) *	1988-08-24	1990-03-01	Behringwerke Ag	Expression funktionsfaehiger homologer und heterologer proteine auf der aeusseren membran von e.coli und anderen gramnegativen bakterien
US5270181A (en) *	1991-02-06	1993-12-14	Genetics Institute, Inc.	Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
US5646016A (en) *	1991-02-06	1997-07-08	Genetics Institute, Inc.	Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
IT1266740B1 (it) *	1994-07-01	1997-01-14	Maria Paola Landini	Materiale proteico ricombinante legante anticorpi contro il citomegalovirus umano, reagenti diagnostici derivati da tale

1996
- 1996-05-14 AT AT96915775T patent/ATE406451T1/de not_active IP Right Cessation
- 1996-05-14 WO PCT/US1996/006841 patent/WO1996036722A2/en not_active Ceased
- 1996-05-14 JP JP53496696A patent/JP4108122B2/ja not_active Expired - Fee Related
- 1996-05-14 CA CA2221192A patent/CA2221192C/en not_active Expired - Fee Related
- 1996-05-14 ES ES96915775T patent/ES2313727T3/es not_active Expired - Lifetime
- 1996-05-14 DE DE69637659T patent/DE69637659D1/de not_active Expired - Lifetime
- 1996-05-14 EP EP96915775A patent/EP0826060B1/de not_active Expired - Lifetime

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Publication number	Publication date
JP4108122B2 (ja)	2008-06-25
WO1996036722A3 (en)	1996-12-19
WO1996036722A2 (en)	1996-11-21
ES2313727T3 (es)	2009-03-01
ATE406451T1 (de)	2008-09-15
JPH11505123A (ja)	1999-05-18
EP0826060A2 (de)	1998-03-04
DE69637659D1 (de)	2008-10-09
EP0826060B1 (de)	2008-08-27
CA2221192A1 (en)	1996-11-21

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2015-06-26	MKLA	Lapsed	Effective date: 20150514

Publication	Publication Date	Title
CA2626423C (en)	2015-01-27	Detection of primary infections with pathogens
EP0408178A1 (de)	1991-01-16	Immobilisierte Variante des Proteins G und ihre Verwendung
JP4612071B2 (ja)	2011-01-12	エプスタイン−バールウイルスペプチド及び該ペプチドに対する抗体
EP0769057B1 (de)	2004-09-01	Rekombinante mono- und polyantigene zum nachweis von cytomegalovirus-spezifischen igm mittels enzymimmunotesten
AU4643693A (en)	1994-01-24	Methods for using cks fusion proteins
WO1994000594A9 (en)	1994-03-17	Methods for using cks fusion proteins
WO1992017588A2 (en)	1992-10-15	METHOD FOR PRODUCTION OF AN IgA BINDING PROTEIN DERIVED FROM GROUP B STREPTOCOCCI
US5965702A (en)	1999-10-12	Borrelia burgdorferi antigens and uses thereof
CN109929040B (zh)	2021-03-23	一种eb病毒bfrf3-bzlf1融合蛋白、基因、包含其的载体、宿主细胞、试纸条及其生产方法和应用
Baylis et al.	1989	The characterization of the EBV alkaline deoxyribonuclease cloned and expressed in E. coli
EP0781846A2 (de)	1997-07-02	Treponema palladium Fusions-Antigen, Nachweis für Anti-treponema palladium Antikörpern mit diesen Fusions-Antigenen
CA2221192C (en)	2011-11-01	Embedded expression of heterologous proteins
EP0316170A2 (de)	1989-05-17	Verfahren zur Identifizierung von in vivo exprimierten Antigenen, diagnostische Tests dafür und Vakzine die diese enthalten
US6120989A (en)	2000-09-19	Isolated human cytomegalovirus polypeptides and uses thereof
Reischl et al.	1996	Expression and purification of an Epstein-Barr virus encoded 23-kDa protein and characterization of its immunological properties
US7901910B2 (en)	2011-03-08	Embedded expression of insoluble heterologous proteins
WO1996032962A1 (en)	1996-10-24	Immunoassay for herpes simplex virus
EP1018016B1 (de)	2002-11-20	Immuntest zum nachweis von menschlichen cytomegalovirus spezifischen igm
JP4163799B2 (ja)	2008-10-08	水痘帯状疱疹ウイルス（ＶＺＶ）の糖タンパク質ｇｐＩＩの免疫反応性領域
CN115073614A (zh)	2022-09-20	一种hcmv重组抗原、其制备方法及应用
CA2194135C (en)	2006-07-18	Recombinant mono and poly antigens to detect cytomegalovirus-specific igm in human sera by enzyme immunoassay
Pompéia et al.	1996	Immunopurification of polyclonal antibodies to recombinant proteins of the same gene family
CA2263439C (en)	2008-12-02	Improved western blot test for the identification of antibodies specific against the hcmv virus
JPH01240190A (ja)	1989-09-25	Ｅｂｖ関連抗原の発現ベクター及びそれを組み込んだ大腸菌
HK1191373B (en)	2018-03-23	Treponema pallidum triplet antigen