CA2223155C - Secretion leader trap cloning method - Google Patents

Secretion leader trap cloning method Download PDF

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Publication number: CA2223155C
Authority: CA; Canada
Prior art keywords: receptor; complementary; cell; host cell; dna
Prior art date: 1995-06-07
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA002223155A

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English (en)

French (fr)

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CA2223155A1 (en

Inventor

Si Lok

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Zymogenetics Inc

Original Assignee

Zymogenetics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-06-07

Filing date

1996-06-04

Publication date

2003-08-12

1996-06-04 Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc

1996-12-19 Publication of CA2223155A1 publication Critical patent/CA2223155A1/en

2003-08-12 Application granted granted Critical

2003-08-12 Publication of CA2223155C publication Critical patent/CA2223155C/en

2016-06-04 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Plant Pathology (AREA)
Bioinformatics & Computational Biology (AREA)
Crystallography & Structural Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA002223155A 1995-06-07 1996-06-04 Secretion leader trap cloning method Expired - Fee Related CA2223155C (en)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US48349395A	1995-06-07	1995-06-07
US47280695A	1995-06-07	1995-06-07
US08/483,493		1995-06-07
US08/472,806		1995-06-07
PCT/US1996/008936 WO1996040904A1 (en)	1995-06-07	1996-06-04	Secretion leader trap cloning method

Publications (2)

Publication Number	Publication Date
CA2223155A1 CA2223155A1 (en)	1996-12-19
CA2223155C true CA2223155C (en)	2003-08-12

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ID=27043933

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Application Number	Title	Priority Date	Filing Date
CA002223155A Expired - Fee Related CA2223155C (en)	1995-06-07	1996-06-04	Secretion leader trap cloning method

Country Status (5)

Country	Link
EP (1)	EP0832209A1 (de)
JP (1)	JPH11506939A (de)
AU (1)	AU6045196A (de)
CA (1)	CA2223155C (de)
WO (1)	WO1996040904A1 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5536637A (en) *	1993-04-07	1996-07-16	Genetics Institute, Inc.	Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
GB9701492D0 (en) *	1997-01-24	1997-03-12	Univ Edinburgh	Transfection of embryonic stem cells
US6066460A (en) *	1997-07-24	2000-05-23	President And Fellows Of Harvard College	Method for cloning secreted proteins
JP3499528B2 (ja)	1997-11-26	2004-02-23	中外製薬株式会社	シグナルシーケンストラップ法
US6103472A (en) *	1998-02-20	2000-08-15	Amgen Inc.	Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
US6150098A (en) *	1998-02-20	2000-11-21	Amgen Inc.	Methods for identifying novel secreted mammalian polypeptides
WO1999049028A1 (en) *	1998-03-23	1999-09-30	Genentech, Inc.	Method of selection for genes encoding secreted and transmembrane proteins
WO1999060113A1 (en)	1998-05-20	1999-11-25	Chugai Seiyaku Kabushiki Kaisha	Novel method for isolating gene
WO2001009310A1 (en) *	1999-07-29	2001-02-08	Berlex Laboratories, Inc.	5'ENRICHED cDNA LIBRARIES AND A YEAST SIGNAL TRAP
EP1417347A4 (de) *	2001-05-02	2005-06-22	Univ South Florida	Vektorsystem zur selektion von sezernierte proteine und membrangebundene proteine codierenden genen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ES2061482T3 (es) *	1986-05-02	1994-12-16	Gist Brocades Nv	Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.
US5817797A (en) *	1988-06-01	1998-10-06	The United States Of America As Represented By The Department Of Health And Human Services	Sequencing DNA; a modification of the polymerase chain reaction
DE3901681A1 (de) *	1989-01-21	1990-07-26	Behringwerke Ag	Signalpeptid fuer die sekretion von peptiden in escherichia coli, verfahren zu seiner gewinnung und seine verwendung
GB8926269D0 (en) *	1989-11-21	1990-01-10	Dynal As	Plasmid
JP2879303B2 (ja) *	1993-01-14	1999-04-05	佑本庶	ｃＤＮＡライブラリーの作製方法、および新規なポリペプチドとそれをコードするＤＮＡ
DE4332463A1 (de) *	1993-09-24	1995-03-30	Boehringer Mannheim Gmbh	Verfahren zur spezifischen Klonierung von Nukleinsäuren

1996
- 1996-06-04 EP EP96918107A patent/EP0832209A1/de not_active Withdrawn
- 1996-06-04 WO PCT/US1996/008936 patent/WO1996040904A1/en not_active Ceased
- 1996-06-04 CA CA002223155A patent/CA2223155C/en not_active Expired - Fee Related
- 1996-06-04 AU AU60451/96A patent/AU6045196A/en not_active Abandoned
- 1996-06-04 JP JP9501386A patent/JPH11506939A/ja active Pending

Also Published As

Publication number	Publication date
CA2223155A1 (en)	1996-12-19
JPH11506939A (ja)	1999-06-22
WO1996040904A1 (en)	1996-12-19
AU6045196A (en)	1996-12-30
EP0832209A1 (de)	1998-04-01

Legal Events

Date	Code	Title	Description
1997-12-02	EEER	Examination request
2011-06-06	MKLA	Lapsed

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