CA2231199C - Dioxetanes 1,2 chemiluminescents a rendement ameliore - Google Patents

Dioxetanes 1,2 chemiluminescents a rendement ameliore Download PDF

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Publication number
CA2231199C
CA2231199C CA002231199A CA2231199A CA2231199C CA 2231199 C CA2231199 C CA 2231199C CA 002231199 A CA002231199 A CA 002231199A CA 2231199 A CA2231199 A CA 2231199A CA 2231199 C CA2231199 C CA 2231199C
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CA
Canada
Prior art keywords
group
alkyl
carbon atoms
enzyme
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002231199A
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English (en)
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CA2231199A1 (fr
Inventor
Irena Bronstein
Brooks Edwards
Alison Sparks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Applera Corp
Applied Biosystems LLC
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/544,172 external-priority patent/US5840919A/en
Priority claimed from US08/547,372 external-priority patent/US5679803A/en
Application filed by Applera Corp, Applied Biosystems LLC, Applied Biosystems Inc filed Critical Applera Corp
Priority claimed from PCT/US1996/014390 external-priority patent/WO1997014692A1/fr
Publication of CA2231199A1 publication Critical patent/CA2231199A1/fr
Application granted granted Critical
Publication of CA2231199C publication Critical patent/CA2231199C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Pyrane Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Une nouvelle classe de dioxétanes stables porte un groupe polycyclique stabilisant et une fraction aryloxy, dont l'atome d'oxygène présente un groupe protecteur pouvant être ôté par une amorce enzymatique ou chimique mélangée au dioxétane. Le groupe polycyclique stabilisant est de préférence le spiroadamantane. Le groupe porte également une fraction alcoxy, aryloxy, aralkyloxy ou cycloalkyloxy partiellement ou complètement substituée par des halogènes, et notamment du fluor et du chlore. Une sélection appropriée de groupes actifs d'électrons sur la fraction stabilisante, le groupe aryle ou le groupe OR permet d'obtenir une cinétique enzymatique accrue, une intensité lumineuse supérieure et une excellente sensibilité de détection dans divers dosages.
CA002231199A 1995-10-17 1996-10-17 Dioxetanes 1,2 chemiluminescents a rendement ameliore Expired - Lifetime CA2231199C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/544,172 US5840919A (en) 1993-05-07 1995-10-17 Chemiluminescent 1,2-dioxetanes
US08/544,172 1995-10-17
US08/547,372 US5679803A (en) 1995-10-25 1995-10-25 1,2 chemiluminescent dioxetanes of improved performance
US08/547,372 1995-10-25
PCT/US1996/014390 WO1997014692A1 (fr) 1995-10-17 1996-10-17 Dioxetanes 1,2 chemiluminescents a rendement ameliore

Publications (2)

Publication Number Publication Date
CA2231199A1 CA2231199A1 (fr) 1997-04-24
CA2231199C true CA2231199C (fr) 2006-04-04

Family

ID=36143342

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002231199A Expired - Lifetime CA2231199C (fr) 1995-10-17 1996-10-17 Dioxetanes 1,2 chemiluminescents a rendement ameliore

Country Status (1)

Country Link
CA (1) CA2231199C (fr)

Also Published As

Publication number Publication date
CA2231199A1 (fr) 1997-04-24

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