CA2235762A1 - Dispositifs integres d'hybridation d'acides nucleiques dont la fonction est fondee sur la chimie des surfaces actives - Google Patents
Dispositifs integres d'hybridation d'acides nucleiques dont la fonction est fondee sur la chimie des surfaces actives Download PDFInfo
- Publication number
- CA2235762A1 CA2235762A1 CA002235762A CA2235762A CA2235762A1 CA 2235762 A1 CA2235762 A1 CA 2235762A1 CA 002235762 A CA002235762 A CA 002235762A CA 2235762 A CA2235762 A CA 2235762A CA 2235762 A1 CA2235762 A1 CA 2235762A1
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- Prior art keywords
- hybridization
- probe
- oligonucleotide probe
- target area
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Le dispositif d'hybridation selon l'invention est constitué d'une sonde oligonucléotidique et d'un substrat solide pourvu d'une surface de support présentant un champ électrostatique neutre ou négatif et d'une surface d'hybridation. Comme le montre la figure, la surface d'hybridation est accessible pour permettre la liaison de la sonde oligonucléotidique au substrat solide. La sonde oligonucléotidique est liée à la surface d'hybridation du substrat solide à une distance ne dépassant pas 100 angströms. L'invention concerne en outre un procédé permettant d'utiliser le dispositif d'hybridation pour détecter des changements simples de bases dans des séquences cibles d'ADN ou d'ARN.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US669695P | 1995-11-14 | 1995-11-14 | |
| US60/006,696 | 1995-11-14 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2235762A1 true CA2235762A1 (fr) | 1997-05-22 |
Family
ID=21722141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002235762A Abandoned CA2235762A1 (fr) | 1995-11-14 | 1996-11-14 | Dispositifs integres d'hybridation d'acides nucleiques dont la fonction est fondee sur la chimie des surfaces actives |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0910570A4 (fr) |
| JP (1) | JP2001508281A (fr) |
| AU (1) | AU7612296A (fr) |
| CA (1) | CA2235762A1 (fr) |
| WO (1) | WO1997018226A1 (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7173011B2 (en) | 2000-11-20 | 2007-02-06 | University Of Southern California | Radiation therapy methods |
| JP2002512045A (ja) * | 1998-04-20 | 2002-04-23 | アフィメトリックス インコーポレイテッド | 核酸プローブアレイに対する非特異的な結合を減少させるための方法 |
| ATE334226T1 (de) * | 1999-01-25 | 2006-08-15 | Micronas Holding Gmbh | Immobilisierung von molekülen auf oberflächen über polymerbürsten |
| WO2001014585A1 (fr) * | 1999-08-24 | 2001-03-01 | Europäisches Laboratorium für Molekularbiologie (EMBL) | Immobilisation et marquage de biopolymeres |
| FR2802550B1 (fr) * | 1999-12-17 | 2004-03-05 | Centre Nat Rech Scient | Biopuces, preparation et utilisations |
| AU6291301A (en) | 2000-02-22 | 2001-09-03 | Genospectra, Inc. | Microarray fabrication techniques and apparatus |
| CN1404415A (zh) | 2000-02-22 | 2003-03-19 | 基因谱公司 | 微阵列制造技术及设备 |
| AU2000269393A1 (en) * | 2000-03-09 | 2001-09-17 | Genometrix Genomix, Inc. | Integrated nucleic acid hybridization devices |
| CA2656209C (fr) * | 2001-07-11 | 2012-11-27 | Baylor College Of Medicine | Procedes et dispositifs bases sur une nouvelle forme de double helice d'acide nucleique sur une surface |
| US20030198967A1 (en) * | 2002-04-23 | 2003-10-23 | Matson Robert S. | Multi-functional microarrays and methods |
| US6984485B2 (en) | 2002-04-23 | 2006-01-10 | Beckman Coulter, Inc. | Polymer-coated substrates for immobilization of biomolecules and cells |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5599667A (en) * | 1987-03-02 | 1997-02-04 | Gen-Probe Incorporated | Polycationic supports and nucleic acid purification separation and hybridization |
| WO1990001564A1 (fr) * | 1988-08-09 | 1990-02-22 | Microprobe Corporation | Procedes d'analyse a cibles multiples par hybridation d'acides nucleiques |
| US5237016A (en) * | 1989-01-05 | 1993-08-17 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
| CA1337639C (fr) * | 1989-08-01 | 1995-11-28 | Joseph Eugene Celebuski | Essai de sondes d'adn faisant appel a des brins de sondes ayant une charge neutre |
| ES2116977T3 (es) * | 1990-05-11 | 1998-08-01 | Microprobe Corp | Soportes solidos para ensayos de hibridacion de acidos nucleicos y metodos para inmovilizar oligonucleotidos de modo covalente. |
| GB9208921D0 (en) * | 1992-04-24 | 1992-06-10 | Isis Innovation | Electrochemical treatment of surfaces |
| WO1996031522A1 (fr) * | 1995-04-03 | 1996-10-10 | New York University | Procede permettant de mesurer des caracteristiques physiques d'acides nucleiques par imagerie microscopique |
-
1996
- 1996-11-14 CA CA002235762A patent/CA2235762A1/fr not_active Abandoned
- 1996-11-14 EP EP96938841A patent/EP0910570A4/fr not_active Withdrawn
- 1996-11-14 JP JP51904597A patent/JP2001508281A/ja active Pending
- 1996-11-14 WO PCT/US1996/018212 patent/WO1997018226A1/fr not_active Ceased
- 1996-11-14 AU AU76122/96A patent/AU7612296A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0910570A1 (fr) | 1999-04-28 |
| JP2001508281A (ja) | 2001-06-26 |
| WO1997018226A1 (fr) | 1997-05-22 |
| EP0910570A4 (fr) | 2002-01-16 |
| AU7612296A (en) | 1997-06-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |