CA2236113C - Vecteur de virus d'arn a brin negatif possedant une activite de replication autonome - Google Patents

Vecteur de virus d'arn a brin negatif possedant une activite de replication autonome Download PDF

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Publication number
CA2236113C
CA2236113C CA002236113A CA2236113A CA2236113C CA 2236113 C CA2236113 C CA 2236113C CA 002236113 A CA002236113 A CA 002236113A CA 2236113 A CA2236113 A CA 2236113A CA 2236113 C CA2236113 C CA 2236113C
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Prior art keywords
rna
complex
gene
virus
host cell
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Expired - Fee Related
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CA002236113A
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English (en)
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CA2236113A1 (fr
Inventor
Yoshiyuki Nagai
Atsushi Kato
Fukashi Murai
Makoto Asakawa
Tsuneaki Sakata
Mamoru Hasegawa
Tatsuo Shioda
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Dnavec Research Inc
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Dnavec Research Inc
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Priority claimed from PCT/JP1996/003068 external-priority patent/WO1997016538A1/fr
Publication of CA2236113A1 publication Critical patent/CA2236113A1/fr
Application granted granted Critical
Publication of CA2236113C publication Critical patent/CA2236113C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86Viral vectors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/36011Togaviridae
    • C12N2770/36111Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141Use of virus, viral particle or viral elements as a vector
    • C12N2770/36143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/36011Togaviridae
    • C12N2770/36111Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36151Methods of production or purification of viral material
    • C12N2770/36152Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un processus permettant de reconstituer des virions de virus sendai, lequel processus consiste à introduire un virus sendai dans un hôte dans lequel les gènes de réplication précoces ont tous été exprimés. Ce processus permet de produire un vecteur de virus d'ARN à brin négatif d'une grande valeur sur le plan pratique.
CA002236113A 1995-10-31 1996-10-22 Vecteur de virus d'arn a brin negatif possedant une activite de replication autonome Expired - Fee Related CA2236113C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP30831595 1995-10-31
JP7/308315 1995-10-31
PCT/JP1996/003068 WO1997016538A1 (fr) 1995-10-31 1996-10-22 Vecteur de virus d'arn a brin negatif possedant une activite de replication autonome

Publications (2)

Publication Number Publication Date
CA2236113A1 CA2236113A1 (fr) 1997-05-09
CA2236113C true CA2236113C (fr) 2007-02-06

Family

ID=37734903

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002236113A Expired - Fee Related CA2236113C (fr) 1995-10-31 1996-10-22 Vecteur de virus d'arn a brin negatif possedant une activite de replication autonome

Country Status (1)

Country Link
CA (1) CA2236113C (fr)

Also Published As

Publication number Publication date
CA2236113A1 (fr) 1997-05-09

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Effective date: 20151022