CA2237473C - Reagent system and method for the differentiation and identification of reticulocytes - Google Patents

Reagent system and method for the differentiation and identification of reticulocytes Download PDF

Info

Publication number: CA2237473C
Authority: CA; Canada
Prior art keywords: gram; reagent; reticulocyte; grams; reticulocytes
Prior art date: 1995-11-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA002237473A

Other languages

English (en)

French (fr)

Other versions

CA2237473A1 (en

Inventor

Robert M. Studholme

Paul N. Marshall

Anne M. Embleton

John G. Glazier

Luc Van Hove

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abbott Laboratories

Original Assignee

Abbott Laboratories

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-11-20

Filing date

1996-11-18

Publication date

2004-11-02

1996-11-18 Application filed by Abbott Laboratories filed Critical Abbott Laboratories

1997-05-29 Publication of CA2237473A1 publication Critical patent/CA2237473A1/en

2004-11-02 Application granted granted Critical

2004-11-02 Publication of CA2237473C publication Critical patent/CA2237473C/en

2016-11-18 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

210000001995 reticulocyte Anatomy 0.000 title claims abstract description 94
239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 69
238000000034 method Methods 0.000 title claims description 47
230000004069 differentiation Effects 0.000 title description 3
210000004369 blood Anatomy 0.000 claims abstract description 35
239000008280 blood Substances 0.000 claims abstract description 35
239000003085 diluting agent Substances 0.000 claims abstract description 26
210000004027 cell Anatomy 0.000 claims abstract description 16
239000000203 mixture Substances 0.000 claims abstract description 15
239000012128 staining reagent Substances 0.000 claims abstract description 13
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 16
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical class OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 12
NZYCYASKVWSANA-UHFFFAOYSA-M new methylene blue Chemical compound [Cl-].CCNC1=C(C)C=C2N=C(C=C(C(NCC)=C3)C)C3=[S+]C2=C1 NZYCYASKVWSANA-UHFFFAOYSA-M 0.000 claims description 9
230000003287 optical effect Effects 0.000 claims description 9
BHATUINFZWUDIX-UHFFFAOYSA-N Zwittergent 3-14 Chemical compound CCCCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O BHATUINFZWUDIX-UHFFFAOYSA-N 0.000 claims description 8
229940061607 dibasic sodium phosphate Drugs 0.000 claims description 8
LVXHNCUCBXIIPE-UHFFFAOYSA-L disodium;hydrogen phosphate;hydrate Chemical compound O.[Na+].[Na+].OP([O-])([O-])=O LVXHNCUCBXIIPE-UHFFFAOYSA-L 0.000 claims description 8
IZWSFJTYBVKZNK-UHFFFAOYSA-N lauryl sulfobetaine Chemical compound CCCCCCCCCCCC[N+](C)(C)CCCS([O-])(=O)=O IZWSFJTYBVKZNK-UHFFFAOYSA-N 0.000 claims description 8
239000001103 potassium chloride Substances 0.000 claims description 8
235000011164 potassium chloride Nutrition 0.000 claims description 8
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 8
239000011780 sodium chloride Substances 0.000 claims description 8
239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 6
239000003795 chemical substances by application Substances 0.000 claims description 6
235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 6
CIYKWVNUXJDNNK-UHFFFAOYSA-N oxazine-750 Chemical compound N1=C2C3=CC=CC=C3C(NCC)=CC2=[O+]C2=C1C=C1CCCN3CCCC2=C13 CIYKWVNUXJDNNK-UHFFFAOYSA-N 0.000 claims description 6
229940100555 2-methyl-4-isothiazolin-3-one Drugs 0.000 claims description 5
229940100484 5-chloro-2-methyl-4-isothiazolin-3-one Drugs 0.000 claims description 5
DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 claims description 5
125000000853 cresyl group Chemical group C1(=CC=C(C=C1)C)* 0.000 claims description 5
239000008367 deionised water Substances 0.000 claims description 5
229910021641 deionized water Inorganic materials 0.000 claims description 5
SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 claims description 5
239000003792 electrolyte Substances 0.000 claims description 5
BEGLCMHJXHIJLR-UHFFFAOYSA-N methylisothiazolinone Chemical compound CN1SC=CC1=O BEGLCMHJXHIJLR-UHFFFAOYSA-N 0.000 claims description 5
108020004707 nucleic acids Proteins 0.000 claims description 5
150000007523 nucleic acids Chemical class 0.000 claims description 5
102000039446 nucleic acids Human genes 0.000 claims description 5
GKASDNZWUGIAMG-UHFFFAOYSA-N triethyl orthoformate Chemical compound CCOC(OCC)OCC GKASDNZWUGIAMG-UHFFFAOYSA-N 0.000 claims description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 4
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
239000000980 acid dye Substances 0.000 claims description 4
IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 claims description 4
150000004682 monohydrates Chemical class 0.000 claims description 4
235000006408 oxalic acid Nutrition 0.000 claims description 4
229910052700 potassium Inorganic materials 0.000 claims description 4
239000011591 potassium Substances 0.000 claims description 4
159000000000 sodium salts Chemical class 0.000 claims description 4
ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims 4
NLEBIOOXCVAHBD-QKMCSOCLSA-N dodecyl beta-D-maltoside Chemical compound O[C@@H]1[C@@H](O)[C@H](OCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 NLEBIOOXCVAHBD-QKMCSOCLSA-N 0.000 claims 4
238000005286 illumination Methods 0.000 claims 1
238000001514 detection method Methods 0.000 abstract description 6
238000000149 argon plasma sintering Methods 0.000 abstract description 3
239000000523 sample Substances 0.000 description 46
238000004458 analytical method Methods 0.000 description 16
210000003743 erythrocyte Anatomy 0.000 description 15
238000005259 measurement Methods 0.000 description 10
108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
239000000975 dye Substances 0.000 description 6
230000004048 modification Effects 0.000 description 6
238000012986 modification Methods 0.000 description 5
-1 n-dodecyl Chemical group 0.000 description 5
239000000243 solution Substances 0.000 description 5
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
210000001772 blood platelet Anatomy 0.000 description 4
238000011534 incubation Methods 0.000 description 4
210000000265 leukocyte Anatomy 0.000 description 4
230000008569 process Effects 0.000 description 4
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
102000001554 Hemoglobins Human genes 0.000 description 3
108010054147 Hemoglobins Proteins 0.000 description 3
208000007502 anemia Diseases 0.000 description 3
238000003556 assay Methods 0.000 description 3
238000013480 data collection Methods 0.000 description 3
230000005284 excitation Effects 0.000 description 3
239000000463 material Substances 0.000 description 3
238000011002 quantification Methods 0.000 description 3
238000007430 reference method Methods 0.000 description 3
QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
OVBJJZOQPCKUOR-UHFFFAOYSA-L EDTA disodium salt dihydrate Chemical compound O.O.[Na+].[Na+].[O-]C(=O)C[NH+](CC([O-])=O)CC[NH+](CC([O-])=O)CC([O-])=O OVBJJZOQPCKUOR-UHFFFAOYSA-L 0.000 description 2
230000008901 benefit Effects 0.000 description 2
238000004364 calculation method Methods 0.000 description 2
125000002091 cationic group Chemical group 0.000 description 2
238000004140 cleaning Methods 0.000 description 2
230000015271 coagulation Effects 0.000 description 2
238000005345 coagulation Methods 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
239000007850 fluorescent dye Substances 0.000 description 2
230000002101 lytic effect Effects 0.000 description 2
238000004519 manufacturing process Methods 0.000 description 2
239000013610 patient sample Substances 0.000 description 2
239000002244 precipitate Substances 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
238000013515 script Methods 0.000 description 2
239000002699 waste material Substances 0.000 description 2
206010002065 Anaemia megaloblastic Diseases 0.000 description 1
208000032467 Aplastic anaemia Diseases 0.000 description 1
206010021143 Hypoxia Diseases 0.000 description 1
208000000682 Megaloblastic Anemia Diseases 0.000 description 1
108700014121 Pyruvate Kinase Deficiency of Red Cells Proteins 0.000 description 1
206010043395 Thalassaemia sickle cell Diseases 0.000 description 1
FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical compound C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
230000003466 anti-cipated effect Effects 0.000 description 1
239000002246 antineoplastic agent Substances 0.000 description 1
238000013459 approach Methods 0.000 description 1
KSCQDDRPFHTIRL-UHFFFAOYSA-N auramine O Chemical compound [H+].[Cl-].C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 KSCQDDRPFHTIRL-UHFFFAOYSA-N 0.000 description 1
DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
210000001185 bone marrow Anatomy 0.000 description 1
230000005983 bone marrow dysfunction Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
238000004422 calculation algorithm Methods 0.000 description 1
210000000170 cell membrane Anatomy 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000008859 change Effects 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
229940127089 cytotoxic agent Drugs 0.000 description 1
238000007405 data analysis Methods 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
150000004683 dihydrates Chemical class 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
238000009472 formulation Methods 0.000 description 1
238000009499 grossing Methods 0.000 description 1
229910052734 helium Inorganic materials 0.000 description 1
239000001307 helium Substances 0.000 description 1
SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
230000002489 hematologic effect Effects 0.000 description 1
208000007475 hemolytic anemia Diseases 0.000 description 1
230000001146 hypoxic effect Effects 0.000 description 1
238000002847 impedance measurement Methods 0.000 description 1
229940060367 inert ingredients Drugs 0.000 description 1
238000012417 linear regression Methods 0.000 description 1
231100001016 megaloblastic anemia Toxicity 0.000 description 1
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
229910052753 mercury Inorganic materials 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
229910052754 neon Inorganic materials 0.000 description 1
GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 description 1
210000003924 normoblast Anatomy 0.000 description 1
239000002245 particle Substances 0.000 description 1
230000002572 peristaltic effect Effects 0.000 description 1
INCIMLINXXICKS-UHFFFAOYSA-M pyronin Y Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2OC3=CC(N(C)C)=CC=C3C=C21 INCIMLINXXICKS-UHFFFAOYSA-M 0.000 description 1
108020004418 ribosomal RNA Proteins 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
208000007056 sickle cell anemia Diseases 0.000 description 1
238000010186 staining Methods 0.000 description 1
239000012192 staining solution Substances 0.000 description 1
230000004936 stimulating effect Effects 0.000 description 1
239000000126 substance Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
ACOJCCLIDPZYJC-UHFFFAOYSA-M thiazole orange Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2C(C=C3N(C4=CC=CC=C4S3)C)=CC=[N+](C)C2=C1 ACOJCCLIDPZYJC-UHFFFAOYSA-M 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
239000002888 zwitterionic surfactant Substances 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Hematology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Urology & Nephrology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)

CA002237473A 1995-11-20 1996-11-18 Reagent system and method for the differentiation and identification of reticulocytes Expired - Fee Related CA2237473C (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US08/560,601		1995-11-20
US08/560,601 US5733784A (en)	1995-11-20	1995-11-20	Reagent system and method for the differentiation and identification of reticulocytes
PCT/US1996/018471 WO1997019356A1 (en)	1995-11-20	1996-11-18	Reagent system and method for the differentiation and identification of reticulocytes

Publications (2)

Publication Number	Publication Date
CA2237473A1 CA2237473A1 (en)	1997-05-29
CA2237473C true CA2237473C (en)	2004-11-02

Family

ID=24238497

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002237473A Expired - Fee Related CA2237473C (en)	1995-11-20	1996-11-18	Reagent system and method for the differentiation and identification of reticulocytes

Country Status (8)

Country	Link
US (1)	US5733784A (de)
EP (1)	EP0864091B1 (de)
JP (1)	JP3725553B2 (de)
AT (1)	ATE249630T1 (de)
CA (1)	CA2237473C (de)
DE (1)	DE69629938T2 (de)
ES (1)	ES2206613T3 (de)
WO (1)	WO1997019356A1 (de)

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EP4086605A1 (de) *	2011-06-17	2022-11-09	Roche Diagnostics Hematology, Inc.	Lösung und verfahren für histologische behandlung biologischer proben

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FR2759166B1 (fr) *	1997-01-31	1999-04-23	Abx Sa	Reactif de coloration pour la determination de cellules sanguines
US5935857A (en) *	1997-08-01	1999-08-10	Coulter International Corp.	Blood diluent
US5874311A (en) *	1997-11-21	1999-02-23	Coulter International Corp.	Method for differentiation of reticulocytes in blood
US6060322A (en) *	1998-10-20	2000-05-09	Coulter International Corp.	Method for identification of reticulated cells
US6271035B1 (en) *	1998-10-20	2001-08-07	Coulter International Corp.	Methods and compositions for rapid staining of nucleic acids in whole cells
JP3886271B2 (ja) *	1998-11-27	2007-02-28	シスメックス株式会社	赤芽球の分類計数用試薬及び分類計数方法
US6444471B1 (en)	1999-10-18	2002-09-03	Research & Diagnostic Systems, Inc.	Reticulocyte containing complete blood control
US6784981B1 (en)	2000-06-02	2004-08-31	Idexx Laboratories, Inc.	Flow cytometry-based hematology system
US6706526B2 (en) *	2002-01-24	2004-03-16	Coulter International Corp.	Low formaldehyde producing blood diluent
CN101349644B (zh) *	2007-07-20	2012-06-27	深圳迈瑞生物医疗电子股份有限公司	一种白细胞分类试剂和其使用方法
US8102161B2 (en) *	2007-09-25	2012-01-24	Tdk Corporation	Stable output in a switching power supply by smoothing the output of the secondary coil
CN101475754A (zh) *	2008-01-04	2009-07-08	深圳迈瑞生物医疗电子股份有限公司	不对称菁类荧光染料,组合物及在生物样品染色中的用途
CN101602762B (zh) *	2008-06-10	2013-10-16	深圳迈瑞生物医疗电子股份有限公司	不对称菁类化合物、其制备方法及应用
CN101726579B (zh) *	2008-10-17	2014-06-18	深圳迈瑞生物医疗电子股份有限公司	血液检测试剂和方法
CN101750274B (zh) *	2008-12-17	2014-06-25	深圳迈瑞生物医疗电子股份有限公司	白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法
CN101988082B (zh) *	2009-07-31	2015-04-08	深圳迈瑞生物医疗电子股份有限公司	白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
IN2014DN10974A (de) *	2012-07-05	2015-09-18	Beckman Coulter Inc
CN111936905B (zh)	2018-03-30	2023-01-06	Idexx实验室公司	流式细胞仪的激光光学组件
EP3789757B1 (de) *	2018-04-28	2025-11-05	Shenzhen Mindray Bio-Medical Electronics Co., Ltd.	Blutanalysegerät und analyseverfahren
KR102547788B1 (ko)	2020-06-17	2023-06-26	아이덱스 래보러토리즈, 인코포레이티드	플로우 사이토미터 및 그 레이저 광학 어셈블리
EP4365570A1 (de) *	2022-11-04	2024-05-08	Horiba, Ltd.	Messverfahren, messsystem und testreagenzienkit

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US4412004A (en) *	1981-06-26	1983-10-25	Technicon Instruments Corporation	Method for treating red blood cells to effect sphering and reagent therefor
US5045472A (en) *	1981-06-26	1991-09-03	Technicon Instruments Corporation	Reagent mixture and composition for treating red blood cells to effect sphering
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US5075556A (en) *	1989-12-01	1991-12-24	Technicon Instruments Corporation	Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5284771A (en) *	1991-12-05	1994-02-08	Miles Inc.	Reagent compositions and their use in sphering cells
US5350695A (en) *	1991-12-05	1994-09-27	Miles Inc.	Methods for the identification and characterization of reticulocytes in whole blood
US5360739A (en) *	1991-12-05	1994-11-01	Miles Inc.	Methods for the identification and characterization of reticulocytes in whole blood
WO1994027146A1 (en) *	1993-05-14	1994-11-24	Coulter Corporation	Reticulocyte analyzing method and apparatus utilizing light scatter techniques
US5487975A (en) *	1993-11-15	1996-01-30	Ventana Medical Systems, Inc.	Biotin/avidin formulation
US5691204A (en) *	1995-04-21	1997-11-25	Abbott Laboratories	Compositions and methods for the rapid analysis of reticulocytes

1995
- 1995-11-20 US US08/560,601 patent/US5733784A/en not_active Expired - Lifetime
1996
- 1996-11-18 ES ES96942767T patent/ES2206613T3/es not_active Expired - Lifetime
- 1996-11-18 DE DE69629938T patent/DE69629938T2/de not_active Expired - Lifetime
- 1996-11-18 AT AT96942767T patent/ATE249630T1/de not_active IP Right Cessation
- 1996-11-18 CA CA002237473A patent/CA2237473C/en not_active Expired - Fee Related
- 1996-11-18 EP EP96942767A patent/EP0864091B1/de not_active Expired - Lifetime
- 1996-11-18 JP JP51982297A patent/JP3725553B2/ja not_active Expired - Fee Related
- 1996-11-18 WO PCT/US1996/018471 patent/WO1997019356A1/en not_active Ceased

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP4086605A1 (de) *	2011-06-17	2022-11-09	Roche Diagnostics Hematology, Inc.	Lösung und verfahren für histologische behandlung biologischer proben

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Publication number	Publication date
US5733784A (en)	1998-03-31
WO1997019356A1 (en)	1997-05-29
CA2237473A1 (en)	1997-05-29
JP2000500584A (ja)	2000-01-18
ATE249630T1 (de)	2003-09-15
JP3725553B2 (ja)	2005-12-14
ES2206613T3 (es)	2004-05-16
EP0864091B1 (de)	2003-09-10
DE69629938D1 (de)	2003-10-16
DE69629938T2 (de)	2004-07-15
EP0864091A1 (de)	1998-09-16

Legal Events

Date	Code	Title	Description
2001-08-30	EEER	Examination request
2012-11-19	MKLA	Lapsed
2012-12-31	MKLA	Lapsed	Effective date: 20121119

Publication	Publication Date	Title
CA2237473C (en)	2004-11-02	Reagent system and method for the differentiation and identification of reticulocytes
JP4366478B2 (ja)	2009-11-18	赤芽球の識別方法
US6114173A (en)	2000-09-05	Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood
AU775260B2 (en)	2004-07-29	Single channel, single dilution detection method
EP0842410B1 (de)	2003-06-11	Zusammensetzungen und verfahren für die schnelle analyse von retikulozyten
JP4433611B2 (ja)	2010-03-17	有核の赤血球の識別方法
EP0797762A1 (de)	1997-10-01	Verfahren zur schnellen und gleichzeitigen analyse nukleierter roter blutzellen
JP2005506525A5 (de)	2006-01-05
EP1032819B1 (de)	2002-07-24	Verfahren zur unterscheidung von reticulocyten in blut
JP2005506525A (ja)	2005-03-03	有核赤血球の計測法の方法
Davis et al.	1990	Clinical flow cytometric reticulocyte analysis
KIM et al.	1998	Simultaneous differentiation and quantitation of erythroblasts and white blood cells on a high throughput clinical haematology analyser
EP0866960B1 (de)	2010-06-30	Verfahren für gleichzeitige analyse von zelllebensfähigkeit, nukleierten roten blutkörperchen und leukozytendifferential
d’Onofrio et al.	1997	Evaluation of the Abbott Cell Dyn 4000 automated fluorescent reticulocyte measurements: comparison with manual, FACScan and Sysmex R1000 methods
EP0259833B1 (de)	1993-01-27	Reagenz und Verfahren zum Klassifizieren von Leukozyten durch Durchfluss-Zytometrie
Abbott et al.	1991	Evaluation of flow cytometric counting procedure for canine reticulocytes by use of thiazole orange
Wearne et al.	1985	Automated enumeration of reticulocytes using acridine orange

CA2237473C - Reagent system and method for the differentiation and identification of reticulocytes - Google Patents

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