CA2244204C - Region nucleaire de fixation d'echafaudage d'une plante accroissant l'expression genique - Google Patents
Region nucleaire de fixation d'echafaudage d'une plante accroissant l'expression genique Download PDFInfo
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- CA2244204C CA2244204C CA002244204A CA2244204A CA2244204C CA 2244204 C CA2244204 C CA 2244204C CA 002244204 A CA002244204 A CA 002244204A CA 2244204 A CA2244204 A CA 2244204A CA 2244204 C CA2244204 C CA 2244204C
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- transcription initiation
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Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La présente invention concerne une région nucléaire de fixation d'échafaudage, laquelle région a été isolée dans un gène de tabac. L'invention concerne également un procédé permettant d'obtenir de cellules de recombinaison présentant des niveaux accrus d'expression de gènes étrangers en leur sein. Le procédé consiste à transformer une cellule avec une structure d'ADN de recombinaison qui comporte, en progressant du 5' au 3', une région d'initiation de transcription, un gène structurel positionné en aval de la région d'initiation de transcription et fonctionnellement associé à cette région, et une région de fixation d'échafaudage de la séquence nucléotique qui y est contenue, soit en position 5' par rapport à la région d'initiation de transcription, soit en position 3' par rapport au gène structurel. L'invention concerne enfin des structures de recombinaison et des vecteurs ADN utilisables pour la mise en oeuvre du procédé de l'invention.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/592,658 US5773695A (en) | 1996-01-26 | 1996-01-26 | Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells |
| US08/592,658 | 1996-01-26 | ||
| PCT/US1997/001278 WO1997027207A1 (fr) | 1996-01-26 | 1997-01-24 | Region nucleaire de fixation d'echafaudage d'une plante accroissant l'expression genique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2244204A1 CA2244204A1 (fr) | 1997-07-31 |
| CA2244204C true CA2244204C (fr) | 2005-10-04 |
Family
ID=35395934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002244204A Expired - Lifetime CA2244204C (fr) | 1996-01-26 | 1997-01-24 | Region nucleaire de fixation d'echafaudage d'une plante accroissant l'expression genique |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2244204C (fr) |
-
1997
- 1997-01-24 CA CA002244204A patent/CA2244204C/fr not_active Expired - Lifetime
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| Publication number | Publication date |
|---|---|
| CA2244204A1 (fr) | 1997-07-31 |
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