CA2260779C - Electrophorese capillaire basee sur la mobilite normalisee - Google Patents
Electrophorese capillaire basee sur la mobilite normalisee Download PDFInfo
- Publication number
- CA2260779C CA2260779C CA002260779A CA2260779A CA2260779C CA 2260779 C CA2260779 C CA 2260779C CA 002260779 A CA002260779 A CA 002260779A CA 2260779 A CA2260779 A CA 2260779A CA 2260779 C CA2260779 C CA 2260779C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- mobility
- species
- marker
- electropherogram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005251 capillar electrophoresis Methods 0.000 title claims abstract description 20
- 230000037230 mobility Effects 0.000 claims abstract description 117
- 239000003550 marker Substances 0.000 claims abstract description 87
- 238000001962 electrophoresis Methods 0.000 claims abstract description 71
- 239000000470 constituent Substances 0.000 claims abstract description 51
- 239000000523 sample Substances 0.000 claims description 99
- 238000000034 method Methods 0.000 claims description 39
- 238000013508 migration Methods 0.000 claims description 36
- 230000005012 migration Effects 0.000 claims description 36
- 238000002835 absorbance Methods 0.000 claims description 22
- 239000013074 reference sample Substances 0.000 claims description 14
- 239000007853 buffer solution Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000000926 separation method Methods 0.000 abstract description 33
- 238000012937 correction Methods 0.000 abstract description 23
- 239000000499 gel Substances 0.000 abstract description 9
- 238000004364 calculation method Methods 0.000 abstract description 2
- 238000011002 quantification Methods 0.000 abstract description 2
- 241000894007 species Species 0.000 description 46
- 238000010606 normalization Methods 0.000 description 36
- 238000001514 detection method Methods 0.000 description 24
- 230000007935 neutral effect Effects 0.000 description 22
- QIAFMBKCNZACKA-UHFFFAOYSA-N N-benzoylglycine Chemical compound OC(=O)CNC(=O)C1=CC=CC=C1 QIAFMBKCNZACKA-UHFFFAOYSA-N 0.000 description 16
- 239000012491 analyte Substances 0.000 description 10
- 238000005370 electroosmosis Methods 0.000 description 9
- 239000013610 patient sample Substances 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 102000009027 Albumins Human genes 0.000 description 6
- 108010088751 Albumins Proteins 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 239000012146 running buffer Substances 0.000 description 5
- 102000004506 Blood Proteins Human genes 0.000 description 4
- 108010017384 Blood Proteins Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 238000005515 capillary zone electrophoresis Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- OZXIZRZFGJZWBF-UHFFFAOYSA-N 1,3,5-trimethyl-2-(2,4,6-trimethylphenoxy)benzene Chemical compound CC1=CC(C)=CC(C)=C1OC1=C(C)C=C(C)C=C1C OZXIZRZFGJZWBF-UHFFFAOYSA-N 0.000 description 2
- LULAYUGMBFYYEX-UHFFFAOYSA-N 3-chlorobenzoic acid Chemical compound OC(=O)C1=CC=CC(Cl)=C1 LULAYUGMBFYYEX-UHFFFAOYSA-N 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- KQNPFQTWMSNSAP-UHFFFAOYSA-N isobutyric acid Chemical compound CC(C)C(O)=O KQNPFQTWMSNSAP-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- SHOJXDKTYKFBRD-UHFFFAOYSA-N mesityl oxide Natural products CC(C)=CC(C)=O SHOJXDKTYKFBRD-UHFFFAOYSA-N 0.000 description 2
- QPJVMBTYPHYUOC-UHFFFAOYSA-N methyl benzoate Chemical compound COC(=O)C1=CC=CC=C1 QPJVMBTYPHYUOC-UHFFFAOYSA-N 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000001121 post-column derivatisation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IKCLCGXPQILATA-UHFFFAOYSA-N 2-chlorobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1Cl IKCLCGXPQILATA-UHFFFAOYSA-N 0.000 description 1
- OUNFHIFSLHEIPK-UHFFFAOYSA-N 2-chloronaphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(S(=O)(=O)O)=C(Cl)C=CC2=C1 OUNFHIFSLHEIPK-UHFFFAOYSA-N 0.000 description 1
- XRHGYUZYPHTUJZ-UHFFFAOYSA-N 4-chlorobenzoic acid Chemical compound OC(=O)C1=CC=C(Cl)C=C1 XRHGYUZYPHTUJZ-UHFFFAOYSA-N 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- LATIUAUOIHKLQY-UHFFFAOYSA-N ClC1=C(C2=C3C(=CC=C2C=C1)C=CC=C3)C(=O)O Chemical compound ClC1=C(C2=C3C(=CC=C2C=C1)C=CC=C3)C(=O)O LATIUAUOIHKLQY-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- -1 N-acetyl methyl ester Chemical class 0.000 description 1
- HHZVGTSZLAQRSM-UHFFFAOYSA-N Phenanthrene-4-carboxylate Chemical compound C1=CC=CC2=C3C(C(=O)O)=CC=CC3=CC=C21 HHZVGTSZLAQRSM-UHFFFAOYSA-N 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000001818 capillary gel electrophoresis Methods 0.000 description 1
- 238000000533 capillary isoelectric focusing Methods 0.000 description 1
- 238000001649 capillary isotachophoresis Methods 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000012886 linear function Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 229940095102 methyl benzoate Drugs 0.000 description 1
- 238000001012 micellar electrokinetic chromatography Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'identification et la quantification des constituants d'un échantillon sont effectuées à l'aide d'électrophérogrammes basés sur la mobilité obtenus à partir de l'électrophorèse capillaire, les mobilités étant normalisées par une correction zéro suivie par la division par la mobilité d'un marqueur sélectionné. La correction zéro est obtenue soit par rapport à un marqueur séparé, soit par un calcul basé sur le même marqueur. Les électrophérogrammes qui en résultent sont plus reconnaissables étant donné qu'ils ressemblent plus étroitement aux motifs de séparation balayés obtenus avec des gels plans, et les constituants individuels de l'échantillon sont identifiés plus facilement et avec plus de fiabilité étant donné que leur position dans l'électrophérogramme est pratiquement constante d'un électrophérogramme à l'autre et, partant, reproductible avec un degré élevé de précision.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/866,282 US5932080A (en) | 1997-05-30 | 1997-05-30 | Mobility-based and normalized capillary electrophoresis |
| US08/866,282 | 1997-05-30 | ||
| PCT/US1998/009930 WO1998054567A1 (fr) | 1997-05-30 | 1998-05-14 | Electrophorese capillaire basee sur la mobilite normalisee |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2260779A1 CA2260779A1 (fr) | 1998-12-03 |
| CA2260779C true CA2260779C (fr) | 2007-01-23 |
Family
ID=37684488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002260779A Expired - Fee Related CA2260779C (fr) | 1997-05-30 | 1998-05-14 | Electrophorese capillaire basee sur la mobilite normalisee |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2260779C (fr) |
-
1998
- 1998-05-14 CA CA002260779A patent/CA2260779C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2260779A1 (fr) | 1998-12-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |