CA2264535A1 - Identification de micro-organismes - Google Patents

Identification de micro-organismes Download PDF

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Publication number
CA2264535A1
CA2264535A1 CA002264535A CA2264535A CA2264535A1 CA 2264535 A1 CA2264535 A1 CA 2264535A1 CA 002264535 A CA002264535 A CA 002264535A CA 2264535 A CA2264535 A CA 2264535A CA 2264535 A1 CA2264535 A1 CA 2264535A1
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CA
Canada
Prior art keywords
spectral data
sample
database
maldi
micro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002264535A
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English (en)
Inventor
Martin Arthur Claydon
Simon Nicholas Davey
Valerie Edward Jones
Derek Boyd Gordon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Manchester Metropolitan University
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2264535A1 publication Critical patent/CA2264535A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Lock And Its Accessories (AREA)

Abstract

La présente invention concerne un procédé et un appareil permettant de caractériser des micro-organismes, au moyen de données spectrales obtenues par spectrométrie de masse à temps de vol à désorption et ionisation laser assistée par matrice (MALDI-TOF-MS) pour une gamme de micro-organismes connus. Ces données spectrales sont incluses dans une base de données comprenant des données spectrales pour tous les micro-organismes connus. On prépare un échantillon d'un micro-organisme non identifié et on l'analyse au moyen des techniques MALDI-TOF-MS afin d'obtenir les données spectrales de ce micro-organisme inconnu. On compare ensuite ces données, par des moyens de comparaison appropriés, aux données spectrales contenues dans la base de données. La similarité indique le genre et l'identité indique le micro-organisme specifique. L'appareil utilisé pour cette analyse présente l'avantage d'être relativement facile d'emploi, une fois installé et étalonné.
CA002264535A 1996-08-27 1997-08-26 Identification de micro-organismes Abandoned CA2264535A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9617852.0 1996-08-27
GBGB9617852.0A GB9617852D0 (en) 1996-08-27 1996-08-27 Micro-organism identification
PCT/GB1997/002278 WO1998009314A1 (fr) 1996-08-27 1997-08-26 Identification de micro-organismes

Publications (1)

Publication Number Publication Date
CA2264535A1 true CA2264535A1 (fr) 1998-03-05

Family

ID=10798972

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002264535A Abandoned CA2264535A1 (fr) 1996-08-27 1997-08-26 Identification de micro-organismes

Country Status (10)

Country Link
EP (1) EP0922295A1 (fr)
JP (1) JP2001502164A (fr)
KR (1) KR20000035935A (fr)
CN (1) CN1234906A (fr)
AU (1) AU4024597A (fr)
BR (1) BR9711448A (fr)
CA (1) CA2264535A1 (fr)
GB (1) GB9617852D0 (fr)
TR (1) TR199900780T2 (fr)
WO (1) WO1998009314A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7348143B2 (en) 2002-03-22 2008-03-25 Phenmenome Discoveries Inc. Method of visualizing non-targeted metabolomic data generated from fourier transform ion cyclotron resonance mass spectrometers
US7349809B2 (en) 2000-02-02 2008-03-25 Yol Bolsum Canada Inc. Method of non-targeted complex sample analysis
CN106399450A (zh) * 2007-01-08 2017-02-15 公共救济事业局-巴黎医院 在种和/或亚种水平鉴定从临床样品分离的菌株的方法
US10697969B2 (en) 2005-09-12 2020-06-30 Med-Life Discoveries Lp Methods for diagnosing a colorectal cancer (CRC) health state or change in CRC health state, or for diagnosing risk of developing CRC or the presence of CRC in a subject

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2335491A (en) * 1998-07-07 1999-09-22 Aromascan Plc Detecting microorganisms
US7020559B1 (en) 1998-11-17 2006-03-28 University Of Maryland Methods for identifying and classifying organisms by mass spectrometry and database searching
WO2001094910A2 (fr) * 2000-06-07 2001-12-13 Basf Aktiengesellschaft Procede d'analyse qualitative et quantitative de melanges complexes de composes chimiques par spectrometrie de masse maldi-tof
CN100489534C (zh) * 2002-04-15 2009-05-20 萨莫芬尼根有限责任公司 生物学分子的定量
DE10300743A1 (de) 2003-01-07 2004-07-29 AnagnosTec, Gesellschaft für Analytische Biochemie und Diagnostik mbH Verfahren zur Identifizierung von Mikroorganismen mittels Massenspektrometrie
DE102004051043B4 (de) * 2004-10-20 2011-06-01 Bruker Daltonik Gmbh Angleichung von Flugzeitmassenspektren
JP4818981B2 (ja) * 2006-04-28 2011-11-16 独立行政法人産業技術総合研究所 細胞の迅速識別方法及び識別装置
EP2060919A1 (fr) * 2007-11-13 2009-05-20 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Matrice MALDI et procédé MALDI
EP2157599A1 (fr) * 2008-08-21 2010-02-24 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Procédé et appareil d'identification de matériaux biologiques
DE102009007266B4 (de) * 2009-02-03 2012-04-19 Bruker Daltonik Gmbh Massenspektrometrische Identifizierung von Mikroorganismen in komplexen Proben
JP2012526996A (ja) * 2009-05-15 2012-11-01 ビオメリュー・インコーポレイテッド 試料内の微生物因子の迅速な同定および／または特徴付けのためのシステムおよび方法
FR2953530A1 (fr) 2009-12-03 2011-06-10 Assist Publ Hopitaux Marseille Procede d'identification rapide des virus par spectrometrie de masse
JP2011147403A (ja) * 2010-01-22 2011-08-04 Hitachi High-Technologies Corp 細菌検査装置及び細菌検査方法
DE102010033105B4 (de) 2010-08-02 2016-05-04 Bruker Daltonik Gmbh Massenspektrometrische Sepsisdiagnose ohne Blutkultur
JP5313218B2 (ja) * 2010-09-30 2013-10-09 株式会社日立ハイテクノロジーズ 細菌検査システム
GB2495899B (en) * 2011-07-04 2018-05-16 Thermo Fisher Scient Bremen Gmbh Identification of samples using a multi pass or multi reflection time of flight mass spectrometer
JP5750676B2 (ja) 2011-10-18 2015-07-22 株式会社島津製作所 細胞識別装置及びプログラム
US9074236B2 (en) * 2012-05-01 2015-07-07 Oxoid Limited Apparatus and methods for microbial identification by mass spectrometry
CN103308696B (zh) * 2013-05-30 2015-06-24 中国疾病预防控制中心传染病预防控制所 基于质谱技术的布鲁氏菌快速检测试剂盒
DE102013022016B4 (de) * 2013-12-20 2015-07-09 Bruker Daltonik Gmbh Mikroben-Identifizierung durch Massenspektrometrie und Infrarot-Spektrometrie
KR20170040078A (ko) 2015-10-02 2017-04-12 숭실대학교산학협력단 식중독균 고속 검출방법
US20170108509A1 (en) * 2015-10-19 2017-04-20 Virgin Instruments Corporation Method For Using Protein Databases To Identify Microorganisms
CN111220686A (zh) * 2018-11-23 2020-06-02 中国科学院大连化学物理研究所 一种基于病毒鉴定的质谱数据库建立方法
KR102028564B1 (ko) * 2018-12-10 2019-10-04 한림대학교 산학협력단 말디-토프 질량분석기를 이용한 메티실린-내성 황색포도구균 균류의 동정방법
CN114184668B (zh) * 2020-09-15 2024-03-26 广州禾信康源医疗科技有限公司 微生物鉴定方法和双极性标准谱图生成方法
CN113219115A (zh) * 2021-05-18 2021-08-06 广东省科学院微生物研究所(广东省微生物分析检测中心) 一种基于maldi-tof ms的蜡样芽胞杆菌和苏云金芽胞杆菌快速鉴定方法

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7349809B2 (en) 2000-02-02 2008-03-25 Yol Bolsum Canada Inc. Method of non-targeted complex sample analysis
US7865312B2 (en) 2000-02-02 2011-01-04 Phenomenome Discoveries Inc. Method of non-targeted complex sample analysis
US7348143B2 (en) 2002-03-22 2008-03-25 Phenmenome Discoveries Inc. Method of visualizing non-targeted metabolomic data generated from fourier transform ion cyclotron resonance mass spectrometers
US10697969B2 (en) 2005-09-12 2020-06-30 Med-Life Discoveries Lp Methods for diagnosing a colorectal cancer (CRC) health state or change in CRC health state, or for diagnosing risk of developing CRC or the presence of CRC in a subject
CN106399450A (zh) * 2007-01-08 2017-02-15 公共救济事业局-巴黎医院 在种和/或亚种水平鉴定从临床样品分离的菌株的方法

Also Published As

Publication number Publication date
JP2001502164A (ja) 2001-02-20
TR199900780T2 (xx) 1999-07-21
WO1998009314A1 (fr) 1998-03-05
EP0922295A1 (fr) 1999-06-16
AU4024597A (en) 1998-03-19
GB9617852D0 (en) 1996-10-09
KR20000035935A (ko) 2000-06-26
CN1234906A (zh) 1999-11-10
BR9711448A (pt) 2000-01-18

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20010827