CA2266847A1 - Compositions et procedes pour l'augmentation de la specificite d'hybridation - Google Patents

Compositions et procedes pour l'augmentation de la specificite d'hybridation Download PDF

Info

Publication number
CA2266847A1
CA2266847A1 CA002266847A CA2266847A CA2266847A1 CA 2266847 A1 CA2266847 A1 CA 2266847A1 CA 002266847 A CA002266847 A CA 002266847A CA 2266847 A CA2266847 A CA 2266847A CA 2266847 A1 CA2266847 A1 CA 2266847A1
Authority
CA
Canada
Prior art keywords
specificity
composition
oligonucleotide
nucleic acid
cation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002266847A
Other languages
English (en)
Inventor
Jeffrey Van Ness
John Tabone
Lori K. Garrison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rapigene Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2266847A1 publication Critical patent/CA2266847A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des compositions et des procédés permettant d'augmenter la spécificité d'un acide nucléique d'une sonde pour un acide nucléique dans une solution d'hybridation. Un résidu abasique, un résidu de désoxyNébularine ou un hybotrope est utilisé pour augmenter la spécificité. L'invention porte sur un procédé d'identification d'hybotropes utiles, dont des sels, des solvants organiques miscibles avec l'eau, des solvants aprotiques et des solvants organiques, selon des critères d'enthalpie. L'hybridation hybotrope et des olignonucléotides modifiés peuvent être utilisés dans des réactions d'amplification, telles que la PCR, des méthodes de séquençage et de dosage génomiques.
CA002266847A 1996-09-24 1997-09-24 Compositions et procedes pour l'augmentation de la specificite d'hybridation Abandoned CA2266847A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US2662196P 1996-09-24 1996-09-24
US71913296A 1996-09-24 1996-09-24
US08/719,132 1996-09-24
US60/026,621 1996-09-24
PCT/US1997/017413 WO1998013527A2 (fr) 1996-09-24 1997-09-24 Compositions et procedes pour l'augmentation de la specificite d'hybridation

Publications (1)

Publication Number Publication Date
CA2266847A1 true CA2266847A1 (fr) 1998-04-02

Family

ID=26701464

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266847A Abandoned CA2266847A1 (fr) 1996-09-24 1997-09-24 Compositions et procedes pour l'augmentation de la specificite d'hybridation

Country Status (5)

Country Link
EP (1) EP0958378A2 (fr)
JP (1) JP2002514909A (fr)
AU (1) AU4599797A (fr)
CA (1) CA2266847A1 (fr)
WO (1) WO1998013527A2 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872521B1 (en) * 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
WO2001023618A2 (fr) * 1999-09-30 2001-04-05 Qiagen Genomics, Inc. Compositions et methodes destinees a reduire la specificite d'hybridation et d'amorçage des oligonucleotides
AU2001289177A1 (en) * 2000-08-30 2002-03-13 Haplogen, Llc Method for determining alleles
EP2801624B1 (fr) 2001-03-16 2019-03-06 Singular Bio, Inc Puces et procédés d'utilisation
US6753145B2 (en) 2001-07-05 2004-06-22 Agilent Technologies, Inc. Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
AU2004235747B2 (en) * 2003-05-01 2009-05-28 Gen-Probe Incorporated Oligonucleotides comprising a molecular switch
CA2494571C (fr) * 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides renfermant des tiges moleculaires
KR100601986B1 (ko) * 2005-02-02 2006-07-18 삼성전자주식회사 유전자를 혼성화하는 방법
KR101110013B1 (ko) * 2007-10-05 2012-02-29 (주)바이오니아 서열 내에 어베이직 부분을 포함하는 ｐｃｒ 증폭용프라이머
WO2017041005A1 (fr) 2015-09-03 2017-03-09 Abbott Molecular Inc. Tampons d'hybridation comprenant un diester d'alkyle
CN108779490A (zh) * 2016-01-08 2018-11-09 雅培分子公司 包含硫氰酸胍的杂交缓冲液
EP4162051A4 (fr) * 2020-06-03 2025-07-09 Quralis Corp Traitement de maladies neurologiques à l'aide de modulateurs de transcrits de gènes
KR102750775B1 (ko) * 2021-09-13 2025-01-09 건국대학교 산학협력단 염 매개의 핵산 고정화 방법, 상기 방법에 따라 고정화된 포획 핵산을 포함하는 고형 지지체, 상기 고형 지지체를 포함하는 측방 유동 분석 스트립, 및 표적 핵산 검출 방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1301606C (fr) * 1986-05-02 1992-05-26 David H. Gillespie Methode chaotropique pour l'evaluation d'acides nucleiques dans un echantillon biologique
CA2031536A1 (fr) * 1989-04-05 1990-10-06 Kevin R. Kearney Reactifs de promotion de l'hybridation
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
EP0529070A1 (fr) * 1991-02-27 1993-03-03 Amoco Corporation Procedes d'amelioration de la sensibilite d'essais d'hybridation
WO1992018649A1 (fr) * 1991-04-12 1992-10-29 Microprobe Corporation Compositions et procedes d'extraction et d'hybridation ameliorees d'acide nucleique
WO1993020234A1 (fr) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company Dosage rapide, a haute capacite, fonde sur l'acide nucleique
JPH06192291A (ja) * 1992-09-07 1994-07-12 Nippon Steel Corp 新規ペプチドとそれを用いた血小板凝集抑制剤及び血液凝固抑制剤
AU4848793A (en) * 1992-09-11 1994-04-12 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
US5545626A (en) * 1994-01-19 1996-08-13 The Trustees Of Columbia University In The City Of New York Method of treating glaucoma with oligonucleotides
WO1995030774A1 (fr) * 1994-05-05 1995-11-16 Beckman Instruments, Inc. Groupements repetes d'oligonucleotides

Also Published As

Publication number Publication date
WO1998013527A2 (fr) 1998-04-02
AU4599797A (en) 1998-04-17
WO1998013527A3 (fr) 1998-08-20
JP2002514909A (ja) 2002-05-21
EP0958378A2 (fr) 1999-11-24

Similar Documents

Publication Publication Date Title
US6361940B1 (en) Compositions and methods for enhancing hybridization and priming specificity
US20240368682A1 (en) Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
US5223414A (en) Process for nucleic acid hybridization and amplification
US6815164B2 (en) Methods and probes for detection and/or quantification of nucleic acid sequences
US6573048B1 (en) Degradable nucleic acid probes and nucleic acid detection methods
US20110003301A1 (en) Methods for detecting genetic variations in dna samples
EP0200362A2 (fr) Procédé pour l'amplification, la détection et/ou le clonage des séquences d'acide nucléique
US20060078936A1 (en) Solid support assay systems and methods utilizing non-standard bases
JP2011239790A (ja) 全ゲノム増幅および遺伝型決定のための方法および組成物
JP2007525963A (ja) 全ゲノム増幅および遺伝型決定のための方法および組成物
KR20040036900A (ko) 닉킹제를 사용한 핵산 단편의 증폭
AU8772798A (en) Improved detection of mutations in nucleic acids by chemical cleavage
EP1169474A1 (fr) Ensemble d'oligonucl otides et ses m thodes d'utilisation
WO2000047766A1 (fr) Methode pour detecter des nucleotides alleliques par amplification multiplex arms
US20040019005A1 (en) Methods for parallel measurement of genetic variations
CA2266847A1 (fr) Compositions et procedes pour l'augmentation de la specificite d'hybridation
EP0952228A2 (fr) Compositions et méthodes pour améliorer la spécificité des hybridations
EP0852263A1 (fr) Procede de concentration d'acide nucleique variant et materiel de test de concentration d'acide nucleique de mise en application du procede
JP2003159100A (ja) 改良された遺伝子の変異検出方法
EP1055735A2 (fr) Méthode pour l'analyse génétique par hybridation en présence d'une protéine de liaison d'ADN double-brin
US20040241697A1 (en) Compositions and methods to identify haplotypes
HK40063948A (en) Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
HK1232917B (en) Methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
HK1219296A1 (zh) 在固相载体扩增核酸的方法
WO2003076665A1 (fr) Tests par hybridation pour analyse de dosage genique

Legal Events

Date Code Title Description
FZDE Discontinued