CA2271228A1 - Technique de production de genes, de proteines et de transcripts marques - Google Patents
Technique de production de genes, de proteines et de transcripts marques Download PDFInfo
- Publication number
- CA2271228A1 CA2271228A1 CA002271228A CA2271228A CA2271228A1 CA 2271228 A1 CA2271228 A1 CA 2271228A1 CA 002271228 A CA002271228 A CA 002271228A CA 2271228 A CA2271228 A CA 2271228A CA 2271228 A1 CA2271228 A1 CA 2271228A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Cette invention porte sur une technique au titre de laquelle un marqueur moléculaire est placé sur un gène, un transcript et une protéine, en un seul processus de recombinaison. Le marqueur de protéine prend la forme d'un peptide unique pouvant être reconnu par un anticorps ou un autre réactif spécifique, le marqueur de transcript, celle d'une séquence nucléotidique codant le peptide qu'une sonde polynucléotidique spécifique est en mesure de reconnaître et le marqueur de gène, celle d'une séquence nucléotidique plus longue renfermant la séquence codant le peptide et d'autres séquences nucléotidiques associées. La particularité la plus notable de l'invention réside essentiellement dans le fait que la structure de l'ADN constitutif du marqueur est telle qu'au moment de son insertion dans un intron à l'intérieur d'un gène, il se crée deux introns hybrides séparés par un nouvel exon codant le marqueur de protéine. Le point fort de cette technique est de permettre l'identification de nouvelles protéines ou de structures contenant une protéine et, cela fait, une identification ainsi qu'une analyse aisées du gène codant ces protéines.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70540496A | 1996-11-08 | 1996-11-08 | |
| US08/705,404 | 1996-11-08 | ||
| PCT/US1997/020150 WO1998020031A1 (fr) | 1996-11-08 | 1997-11-07 | Technique de production de genes, de proteines et de transcripts marques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2271228A1 true CA2271228A1 (fr) | 1998-05-14 |
Family
ID=24833313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002271228A Abandoned CA2271228A1 (fr) | 1996-11-08 | 1997-11-07 | Technique de production de genes, de proteines et de transcripts marques |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU5168598A (fr) |
| CA (1) | CA2271228A1 (fr) |
| WO (1) | WO1998020031A1 (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6207371B1 (en) | 1996-10-04 | 2001-03-27 | Lexicon Genetics Incorporated | Indexed library of cells containing genomic modifications and methods of making and utilizing the same |
| US7332338B2 (en) | 1996-10-04 | 2008-02-19 | Lexicon Pharmaceuticals, Inc. | Vectors for making genomic modifications |
| US6136566A (en) * | 1996-10-04 | 2000-10-24 | Lexicon Graphics Incorporated | Indexed library of cells containing genomic modifications and methods of making and utilizing the same |
| US6855545B1 (en) | 1996-10-04 | 2005-02-15 | Lexicon Genetics Inc. | Indexed library of cells containing genomic modifications and methods of making and utilizing the same |
| US6436707B1 (en) | 1998-03-27 | 2002-08-20 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
| US6080576A (en) * | 1998-03-27 | 2000-06-27 | Lexicon Genetics Incorporated | Vectors for gene trapping and gene activation |
| US6808921B1 (en) | 1998-03-27 | 2004-10-26 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
| JP3688118B2 (ja) * | 1998-05-22 | 2005-08-24 | 独立行政法人科学技術振興機構 | 遺伝子トラップ用ベクターと、このベクターを用いた遺伝子トラップ方法 |
| WO2002053732A2 (fr) * | 2000-12-28 | 2002-07-11 | Pangenex | Technique d'elaboration de bibliotheque de polynucleotides, de reseaux de polynucleotides et de bibliotheques de cellules destines a l'analyse des caracteristiques genomiques de haut rendement |
| EP2245163A2 (fr) * | 2008-01-24 | 2010-11-03 | Yeda Research And Development Company Ltd. | Populations cellulaires pour analyse de polypeptides et utilisations correspondantes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5652128A (en) * | 1993-01-05 | 1997-07-29 | Jarvik; Jonathan Wallace | Method for producing tagged genes, transcripts, and proteins |
-
1997
- 1997-11-07 AU AU51685/98A patent/AU5168598A/en not_active Abandoned
- 1997-11-07 WO PCT/US1997/020150 patent/WO1998020031A1/fr not_active Ceased
- 1997-11-07 CA CA002271228A patent/CA2271228A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU5168598A (en) | 1998-05-29 |
| WO1998020031A1 (fr) | 1998-05-14 |
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