CA2271697C - Variantes d'activateur plasminogene de type tissulaire (t-pa): compositions et techniques d'utilisation - Google Patents

Variantes d'activateur plasminogene de type tissulaire (t-pa): compositions et techniques d'utilisation Download PDF

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Publication number
CA2271697C
CA2271697C CA002271697A CA2271697A CA2271697C CA 2271697 C CA2271697 C CA 2271697C CA 002271697 A CA002271697 A CA 002271697A CA 2271697 A CA2271697 A CA 2271697A CA 2271697 C CA2271697 C CA 2271697C
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CA
Canada
Prior art keywords
protein variant
ser
protein
variant
single chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA002271697A
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English (en)
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CA2271697A1 (fr
Inventor
Edwin L. Madison
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Scripps Research Institute
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Scripps Research Institute
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Publication of CA2271697A1 publication Critical patent/CA2271697A1/fr
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Publication of CA2271697C publication Critical patent/CA2271697C/fr
Anticipated expiration legal-status Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des variantes du facteur plasminogène tissulaire qui font preuve d'une amélioration notable de la stimulation de la fibrine, d'une discrimination considérablement accrue entre cofacteurs de la fibrine, d'une résistance marquée à l'inhibition par PAI-1, et d'une zymogénicité sensiblement accrue, une combinaison de propriétés qui renforcent l'utilité thérapeutique de l'enzyme.
CA002271697A 1996-11-12 1997-11-12 Variantes d'activateur plasminogene de type tissulaire (t-pa): compositions et techniques d'utilisation Expired - Fee Related CA2271697C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3065596P 1996-11-12 1996-11-12
US60/030,655 1996-11-12
PCT/US1997/020226 WO1998021320A2 (fr) 1996-11-12 1997-11-12 VARIANTES D'ACTIVATEUR PLASMINOGENE DE TYPE TISSULAIRE (t-PA): COMPOSITIONS ET TECHNIQUES D'UTILISATION

Publications (2)

Publication Number Publication Date
CA2271697A1 CA2271697A1 (fr) 1998-05-22
CA2271697C true CA2271697C (fr) 2007-10-23

Family

ID=21855292

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002271697A Expired - Fee Related CA2271697C (fr) 1996-11-12 1997-11-12 Variantes d'activateur plasminogene de type tissulaire (t-pa): compositions et techniques d'utilisation

Country Status (6)

Country Link
EP (1) EP0950095A2 (fr)
JP (1) JP2001505765A (fr)
CN (1) CN1244894A (fr)
AU (1) AU735519B2 (fr)
CA (1) CA2271697C (fr)
WO (1) WO1998021320A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8864398A (en) * 1997-08-13 1999-03-08 Boehringer Mannheim Gmbh Plasminogen activator with enhanced zymogenic power and reduced fibrin linking
WO2002072786A2 (fr) 2001-03-13 2002-09-19 Corvas International, Inc. Molecules d'acides nucleiques codant pour une serine protease transmembranaire 7, polypeptides codes et procedes associes
AU2002254357A1 (en) 2001-03-22 2002-10-08 Dendreon Corporation Nucleic acid molecules encoding serine protease cvsp14, the encoded polypeptides and methods based thereon
CA2442089A1 (fr) 2001-03-27 2002-10-03 Dendreon San Diego Llc Molecules d'acide nucleique codant une serine protease transmembranaire 9, polypeptides codes et procedes fondes sur ces derniers
US7112430B2 (en) 2001-05-14 2006-09-26 Dendreon Corporation Nucleic acid molecules encoding a transmembrane serine protease 10, the encoded polypeptides and methods based thereon
US10160813B2 (en) * 2013-01-22 2018-12-25 University Of Tennessee Research Foundation Tissue plasminogen activator antibodies and methods of use
CN114736948B (zh) * 2022-06-10 2022-11-08 深圳市帝迈生物技术有限公司 一种α2-抗纤溶酶活性测定试剂盒
CN117327682A (zh) * 2023-10-07 2024-01-02 福州大学 一种兼顾高效性与安全性的新型溶栓剂的设计及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR861037B (en) * 1985-04-22 1986-07-16 Genentech Inc Novel human tissue plasminogen activator mutants
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties

Also Published As

Publication number Publication date
WO1998021320A2 (fr) 1998-05-22
AU735519B2 (en) 2001-07-12
CN1244894A (zh) 2000-02-16
CA2271697A1 (fr) 1998-05-22
EP0950095A2 (fr) 1999-10-20
WO1998021320A3 (fr) 1998-07-09
AU5585098A (en) 1998-06-03
JP2001505765A (ja) 2001-05-08

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