CA2275892A1 - Constructions d'acide nucleique et leur utilisation en vue d'une incorporation directe dans les cellules - Google Patents
Constructions d'acide nucleique et leur utilisation en vue d'une incorporation directe dans les cellules Download PDFInfo
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- CA2275892A1 CA2275892A1 CA002275892A CA2275892A CA2275892A1 CA 2275892 A1 CA2275892 A1 CA 2275892A1 CA 002275892 A CA002275892 A CA 002275892A CA 2275892 A CA2275892 A CA 2275892A CA 2275892 A1 CA2275892 A1 CA 2275892A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
L'invention concerne des compositions permettant d'incorporer de l'acide nucléique dans une cellule. Des constructions transgéniques comprenant une séquence désirée d'acide nucléique peuvent être injectées dans une cellule avec une protéine afin d'effectuer une incorporation dirigée ou aléatoire de la séquence d'acide nucléique dans l'ADN chromosomique de la cellule. La construction transgénique et la protéine sont incluses dans les compositions décrites, la protéine facilitant l'incorporation de la séquence d'acide nucléique au sein de la construction transgénique, directement dans l'acide nucléique d'une cellule de manière dirigée. Dans d'autres modes de réalisation, les compositions comportent une construction transgénique et une construction d'expression, ladite construction d'expression présentant une séquence d'acide nucléique codant une enzyme capable de faciliter l'incorporation de la séquence transgénique considérée dans une cellule. L'invention a trait aussi aux procédés visant à incorporer un acide nucléique étranger dans une cellule par micro-injection. Dans des modes particuliers de réalisation, le procédé peut être utilisé pour introduire de l'acide nucléique étranger dans des cellules qui se développent dans un état de non adhérence. Dans ce cadre, les cellules sont immobilisées sur une surface d'un substrat incluant une molécule adhérente, telle que la fribronectine. Selon une autre solution, les cellules peuvent être suffisamment stabilisées pour permettre la micro-injection au moyen de pipettes ou par stimulation des cellules elles-mêmes de sorte à exprimer des molécules sur leur surface liant les entités chimiques comprises sur la surface d'une plaque de culture.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3381696P | 1996-12-23 | 1996-12-23 | |
| US60/033,816 | 1996-12-23 | ||
| PCT/US1997/024236 WO1998028417A1 (fr) | 1996-12-23 | 1997-12-20 | Constructions d'acide nucleique et leur utilisation en vue d'une incorporation directe dans les cellules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2275892A1 true CA2275892A1 (fr) | 1998-07-02 |
Family
ID=21872610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002275892A Abandoned CA2275892A1 (fr) | 1996-12-23 | 1997-12-20 | Constructions d'acide nucleique et leur utilisation en vue d'une incorporation directe dans les cellules |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0946718A1 (fr) |
| JP (1) | JP2001507230A (fr) |
| AU (1) | AU733715B2 (fr) |
| CA (1) | CA2275892A1 (fr) |
| WO (1) | WO1998028417A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262668C (zh) * | 1999-01-08 | 2006-07-05 | 阿马克萨有限公司 | 转移核酸通过核被膜的细胞转运系统的用途 |
| EP1319713B1 (fr) | 2000-09-20 | 2010-01-27 | Takara Bio Inc. | Complexe |
| EP1795593A4 (fr) * | 2004-08-24 | 2009-07-08 | Univ Kyoto | Acide nucleique cible d'integration retrovirale |
| US10610606B2 (en) | 2018-02-01 | 2020-04-07 | Homology Medicines, Inc. | Adeno-associated virus compositions for PAH gene transfer and methods of use thereof |
| AU2019215152A1 (en) | 2018-02-01 | 2020-08-20 | Homology Medicines, Inc. | Adeno-associated virus compositions for restoring PAH gene function and methods of use thereof |
| JP7244547B2 (ja) | 2018-02-19 | 2023-03-22 | ホモロジー・メディシンズ・インコーポレイテッド | Ｆ８遺伝子機能を回復させるためのアデノ随伴ウイルス組成物及びその使用の方法 |
| TW202140791A (zh) | 2020-01-13 | 2021-11-01 | 美商霍蒙拉奇醫藥公司 | 治療苯酮尿症之方法 |
| TW202208632A (zh) | 2020-05-27 | 2022-03-01 | 美商同源醫藥公司 | 用於恢復ｐａｈ基因功能的腺相關病毒組成物及其使用方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995032225A1 (fr) * | 1994-05-23 | 1995-11-30 | The Salk Institute For Biological Studies | Procede d'integration en des sites specifiques d'acides nucleiques, et produits associes |
| US5843742A (en) * | 1994-12-16 | 1998-12-01 | Avigen Incorporated | Adeno-associated derived vector systems for gene delivery and integration into target cells |
-
1997
- 1997-12-20 CA CA002275892A patent/CA2275892A1/fr not_active Abandoned
- 1997-12-20 WO PCT/US1997/024236 patent/WO1998028417A1/fr not_active Ceased
- 1997-12-20 AU AU58121/98A patent/AU733715B2/en not_active Ceased
- 1997-12-20 JP JP52911798A patent/JP2001507230A/ja active Pending
- 1997-12-20 EP EP97954318A patent/EP0946718A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP0946718A1 (fr) | 1999-10-06 |
| AU733715B2 (en) | 2001-05-24 |
| JP2001507230A (ja) | 2001-06-05 |
| WO1998028417A1 (fr) | 1998-07-02 |
| AU5812198A (en) | 1998-07-17 |
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