CA2277371C - Milieux de soutien siliceux pour l'immobilisation d'enzymes - Google Patents

Milieux de soutien siliceux pour l'immobilisation d'enzymes Download PDF

Info

Publication number: CA2277371C
Authority: CA; Canada
Prior art keywords: enzyme; support material; linking agent; cross; immobilized
Prior art date: 1999-07-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA002277371A

Other languages

English (en)

Other versions

CA2277371A1 (fr

Inventor

Gerard Le Fevre

Bradley A. Saville

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

1484667 ONTARIO Ltd

Original Assignee

1484667 ONTARIO Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-07-08

Filing date

1999-07-08

Publication date

2005-05-10

1999-07-08 Application filed by 1484667 ONTARIO Ltd filed Critical 1484667 ONTARIO Ltd

1999-07-08 Priority to CA002277371A priority Critical patent/CA2277371C/fr

2001-01-08 Publication of CA2277371A1 publication Critical patent/CA2277371A1/fr

2005-05-10 Application granted granted Critical

2005-05-10 Publication of CA2277371C publication Critical patent/CA2277371C/fr

2019-07-08 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

102000004190 Enzymes Human genes 0.000 title claims abstract description 95
108090000790 Enzymes Proteins 0.000 title claims abstract description 95
238000000034 method Methods 0.000 claims abstract description 34
108010093096 Immobilized Enzymes Proteins 0.000 claims abstract description 24
239000003431 cross linking reagent Substances 0.000 claims abstract description 24
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 18
239000000463 material Substances 0.000 claims description 27
102000003425 Tyrosinase Human genes 0.000 claims description 24
108060008724 Tyrosinase Proteins 0.000 claims description 24
HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical class O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 22
230000000694 effects Effects 0.000 claims description 17
239000007864 aqueous solution Substances 0.000 claims description 11
229910000503 Na-aluminosilicate Inorganic materials 0.000 claims description 9
229920001744 Polyaldehyde Polymers 0.000 claims description 9
235000012217 sodium aluminium silicate Nutrition 0.000 claims description 9
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 8
239000000429 sodium aluminium silicate Substances 0.000 claims description 8
URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims description 8
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
229910000323 aluminium silicate Inorganic materials 0.000 claims description 5
125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 5
239000000741 silica gel Substances 0.000 claims description 5
229910002027 silica gel Inorganic materials 0.000 claims description 5
239000003153 chemical reaction reagent Substances 0.000 claims description 4
238000010168 coupling process Methods 0.000 claims description 4
150000001299 aldehydes Chemical group 0.000 claims description 3
230000008878 coupling Effects 0.000 claims description 3
238000005859 coupling reaction Methods 0.000 claims description 3
230000003100 immobilizing effect Effects 0.000 claims 1
238000004519 manufacturing process Methods 0.000 abstract description 17
230000008569 process Effects 0.000 abstract description 16
WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 abstract description 11
WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 abstract description 11
239000003814 drug Substances 0.000 abstract description 3
239000000126 substance Substances 0.000 abstract description 3
230000001404 mediated effect Effects 0.000 abstract description 2
230000002255 enzymatic effect Effects 0.000 abstract 1
229940088598 enzyme Drugs 0.000 description 80
239000000243 solution Substances 0.000 description 23
239000010457 zeolite Substances 0.000 description 22
229910021536 Zeolite Inorganic materials 0.000 description 16
239000012528 membrane Substances 0.000 description 13
238000006243 chemical reaction Methods 0.000 description 10
239000008363 phosphate buffer Substances 0.000 description 8
239000007853 buffer solution Substances 0.000 description 7
230000002779 inactivation Effects 0.000 description 7
YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 6
229920002302 Nylon 6,6 Polymers 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 5
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
239000004677 Nylon Substances 0.000 description 4
238000013459 approach Methods 0.000 description 4
239000000499 gel Substances 0.000 description 4
239000000203 mixture Substances 0.000 description 4
229920001778 nylon Polymers 0.000 description 4
238000003828 vacuum filtration Methods 0.000 description 4
-1 aluminosilicates Chemical class 0.000 description 3
239000000404 calcium aluminium silicate Substances 0.000 description 3
235000012215 calcium aluminium silicate Nutrition 0.000 description 3
WNCYAPRTYDMSFP-UHFFFAOYSA-N calcium aluminosilicate Chemical compound [Al+3].[Al+3].[Ca+2].[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O WNCYAPRTYDMSFP-UHFFFAOYSA-N 0.000 description 3
229940078583 calcium aluminosilicate Drugs 0.000 description 3
239000007979 citrate buffer Substances 0.000 description 3
238000004132 cross linking Methods 0.000 description 3
238000006911 enzymatic reaction Methods 0.000 description 3
230000004048 modification Effects 0.000 description 3
238000012986 modification Methods 0.000 description 3
150000004760 silicates Chemical class 0.000 description 3
238000003756 stirring Methods 0.000 description 3
239000000758 substrate Substances 0.000 description 3
229960004441 tyrosine Drugs 0.000 description 3
TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 2
PLIKAWJENQZMHA-UHFFFAOYSA-N 4-aminophenol Chemical compound NC1=CC=C(O)C=C1 PLIKAWJENQZMHA-UHFFFAOYSA-N 0.000 description 2
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
229920001661 Chitosan Polymers 0.000 description 2
102000008186 Collagen Human genes 0.000 description 2
108010035532 Collagen Proteins 0.000 description 2
QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 2
102000010911 Enzyme Precursors Human genes 0.000 description 2
108010062466 Enzyme Precursors Proteins 0.000 description 2
239000001263 FEMA 3042 Substances 0.000 description 2
108010015776 Glucose oxidase Proteins 0.000 description 2
239000004366 Glucose oxidase Substances 0.000 description 2
229920002292 Nylon 6 Polymers 0.000 description 2
LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 2
229920001131 Pulp (paper) Polymers 0.000 description 2
HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical group C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 2
229920001436 collagen Polymers 0.000 description 2
230000000052 comparative effect Effects 0.000 description 2
238000001914 filtration Methods 0.000 description 2
125000000524 functional group Chemical group 0.000 description 2
229940116332 glucose oxidase Drugs 0.000 description 2
235000019420 glucose oxidase Nutrition 0.000 description 2
238000004128 high performance liquid chromatography Methods 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
229910052751 metal Inorganic materials 0.000 description 2
239000002184 metal Substances 0.000 description 2
238000002156 mixing Methods 0.000 description 2
239000000843 powder Substances 0.000 description 2
238000012545 processing Methods 0.000 description 2
238000001179 sorption measurement Methods 0.000 description 2
229920002258 tannic acid Polymers 0.000 description 2
LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 2
229940033123 tannic acid Drugs 0.000 description 2
235000015523 tannic acid Nutrition 0.000 description 2
238000012546 transfer Methods 0.000 description 2
238000004065 wastewater treatment Methods 0.000 description 2
239000002023 wood Substances 0.000 description 2
WGAOZGUUHIBABN-UHFFFAOYSA-N 1-aminopentan-1-ol Chemical compound CCCCC(N)O WGAOZGUUHIBABN-UHFFFAOYSA-N 0.000 description 1
YVLHRYOHNHUVOA-UHFFFAOYSA-N 2,2-dimethylpropane-1,1-diamine Chemical compound CC(C)(C)C(N)N YVLHRYOHNHUVOA-UHFFFAOYSA-N 0.000 description 1
HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
235000001674 Agaricus brunnescens Nutrition 0.000 description 1
102000016938 Catalase Human genes 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
102000030523 Catechol oxidase Human genes 0.000 description 1
108010031396 Catechol oxidase Proteins 0.000 description 1
108010059892 Cellulase Proteins 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
101000952234 Homo sapiens Sphingolipid delta(4)-desaturase DES1 Proteins 0.000 description 1
IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
235000010830 Prunus insititia Nutrition 0.000 description 1
244000039376 Prunus insititia Species 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
102100037416 Sphingolipid delta(4)-desaturase DES1 Human genes 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
240000006365 Vitis vinifera Species 0.000 description 1
XELHQSWRNMBGPK-UHFFFAOYSA-N acetaldehyde;diphenylmethanediamine Chemical compound CC=O.C=1C=CC=CC=1C(N)(N)C1=CC=CC=C1 XELHQSWRNMBGPK-UHFFFAOYSA-N 0.000 description 1
239000002253 acid Substances 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
125000005264 aryl amine group Chemical group 0.000 description 1
230000004888 barrier function Effects 0.000 description 1
238000010923 batch production Methods 0.000 description 1
230000001588 bifunctional effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
238000004061 bleaching Methods 0.000 description 1
239000011449 brick Substances 0.000 description 1
238000005282 brightening Methods 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
229940106157 cellulase Drugs 0.000 description 1
239000000919 ceramic Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
150000001875 compounds Chemical group 0.000 description 1
229910052802 copper Inorganic materials 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
238000003795 desorption Methods 0.000 description 1
ZLFRJHOBQVVTOJ-UHFFFAOYSA-N dimethyl hexanediimidate Chemical class COC(=N)CCCCC(=N)OC ZLFRJHOBQVVTOJ-UHFFFAOYSA-N 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
JMIAPORGEDIDLT-UHFFFAOYSA-N ethyl ethanimidate Chemical compound CCOC(C)=N JMIAPORGEDIDLT-UHFFFAOYSA-N 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
239000000174 gluconic acid Substances 0.000 description 1
235000012208 gluconic acid Nutrition 0.000 description 1
239000008103 glucose Substances 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
231100001261 hazardous Toxicity 0.000 description 1
239000012535 impurity Substances 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
150000002527 isonitriles Chemical class 0.000 description 1
230000007774 longterm Effects 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
239000003094 microcapsule Substances 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
150000002978 peroxides Chemical class 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
QIIPQYDSKRYMFG-UHFFFAOYSA-N phenyl hydrogen carbonate Chemical compound OC(=O)OC1=CC=CC=C1 QIIPQYDSKRYMFG-UHFFFAOYSA-N 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
239000005373 porous glass Substances 0.000 description 1
239000011148 porous material Substances 0.000 description 1
230000009979 protective mechanism Effects 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
238000011160 research Methods 0.000 description 1
FZHAPNGMFPVSLP-UHFFFAOYSA-N silanamine Chemical class [SiH3]N FZHAPNGMFPVSLP-UHFFFAOYSA-N 0.000 description 1
238000002791 soaking Methods 0.000 description 1
239000002904 solvent Substances 0.000 description 1
241000894007 species Species 0.000 description 1
238000003860 storage Methods 0.000 description 1
230000009466 transformation Effects 0.000 description 1
239000012588 trypsin Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/225—Tyrosine; 3,4-Dihydroxyphenylalanine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/18—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with another compound as one donor, and incorporation of one atom of oxygen (1.14.18)
- C12Y114/18001—Tyrosinase (1.14.18.1)

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biomedical Technology (AREA)
Inorganic Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

CA002277371A 1999-07-08 1999-07-08 Milieux de soutien siliceux pour l'immobilisation d'enzymes Expired - Fee Related CA2277371C (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA002277371A CA2277371C (fr)	1999-07-08	1999-07-08	Milieux de soutien siliceux pour l'immobilisation d'enzymes

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA002277371A CA2277371C (fr)	1999-07-08	1999-07-08	Milieux de soutien siliceux pour l'immobilisation d'enzymes

Publications (2)

Publication Number	Publication Date
CA2277371A1 CA2277371A1 (fr)	2001-01-08
CA2277371C true CA2277371C (fr)	2005-05-10

Family

ID=4163729

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002277371A Expired - Fee Related CA2277371C (fr)	1999-07-08	1999-07-08	Milieux de soutien siliceux pour l'immobilisation d'enzymes

Country Status (1)

Country	Link
CA (1)	CA2277371C (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN108603177A (zh) *	2015-12-03	2018-09-28	纳幕尔杜邦公司	酶递送系统

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US9303256B2 (en)	2005-09-30	2016-04-05	Novozymes A/S	Immobilization of enzymes
WO2014067933A1 (fr)	2012-10-31	2014-05-08	C-Lecta Gmbh	Préparation de support bioactif pour une sécurité améliorée dans les produits de soin et les aliments
CA2910477C (fr) *	2013-04-26	2021-03-30	Andrew Desbarats	Agent de soutenement, fluide de fracturation rendu visqueux, et procede de fracturation pour extraire du petrole et du gaz

1999
- 1999-07-08 CA CA002277371A patent/CA2277371C/fr not_active Expired - Fee Related

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN108603177A (zh) *	2015-12-03	2018-09-28	纳幕尔杜邦公司	酶递送系统
CN108603177B (zh) *	2015-12-03	2022-06-17	纳幕尔杜邦公司	酶递送系统

Also Published As

Publication number	Publication date
CA2277371A1 (fr)	2001-01-08

Legal Events

Date	Code	Title	Description
1999-07-08	EEER	Examination request
2003-01-15	FZDC	Correction of dead application (reinstatement)
2016-08-20	MKLA	Lapsed	Effective date: 20160708

Publication	Publication Date	Title
US5998183A (en)	1999-12-07	Enzyme immobilization on a siliceous support with a polyaldehyde cross-linking agent
FI85384C (fi)	1992-04-10	Foerfarande foer hydrolysering av hemicellulosa med immobiliserade enzymer och produkt som omfattar ett immobiliserat hemicellulolytiskt enzym.
FI92074C (fi)	1994-09-26	Menetelmä entsyymien immobilisoimiseksi kiinnittämällä ne rakeiseen piimaahan
Isgrove et al.	2001	Enzyme immobilization on nylon–optimization and the steps used to prevent enzyme leakage from the support
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