CA2279131C - Separation electrophoretique des acides nucleiques des proteines a faible ph - Google Patents

Separation electrophoretique des acides nucleiques des proteines a faible ph Download PDF

Info

Publication number: CA2279131C
Authority: CA; Canada
Prior art keywords: nucleic acids; proteins; electrophoresis; rna; protein
Prior art date: 1998-08-04
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA2279131A

Other languages

English (en)

Other versions

CA2279131A1 (fr

Inventor

Carol Ann Kreader

John Wesley Backus

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Ortho Clinical Diagnostics Inc

Original Assignee

Ortho Clinical Diagnostics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-08-04

Filing date

1999-07-29

Publication date

2010-12-14

1999-07-29 Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc

2000-02-04 Publication of CA2279131A1 publication Critical patent/CA2279131A1/fr

2010-12-14 Application granted granted Critical

2010-12-14 Publication of CA2279131C publication Critical patent/CA2279131C/fr

2019-07-29 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Crystallography & Structural Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Saccharide Compounds (AREA)
Electrostatic Separation (AREA)

CA2279131A 1998-08-04 1999-07-29 Separation electrophoretique des acides nucleiques des proteines a faible ph Expired - Fee Related CA2279131C (fr)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US9525898P	1998-08-04	1998-08-04
US60/095,258		1998-08-04
US09/349,387 US6699986B2 (en)	1998-08-04	1999-07-09	Electrophoretic separation of nucleic acids from proteins at low ph
US09/349,387		1999-09-09

Publications (2)

Publication Number	Publication Date
CA2279131A1 CA2279131A1 (fr)	2000-02-04
CA2279131C true CA2279131C (fr)	2010-12-14

Family

ID=26790014

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2279131A Expired - Fee Related CA2279131C (fr)	1998-08-04	1999-07-29	Separation electrophoretique des acides nucleiques des proteines a faible ph

Country Status (6)

Country	Link
US (1)	US6699986B2 (fr)
EP (1)	EP0979868B1 (fr)
JP (1)	JP4531888B2 (fr)
AT (1)	ATE449843T1 (fr)
CA (1)	CA2279131C (fr)
DE (1)	DE69941655D1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU2003302418A1 (en) *	2002-11-28	2004-06-18	Arkray Inc.	Method and apparatus for concentration and purification of nucleic acid
CN100491391C (zh) *	2002-11-28	2009-05-27	爱科来株式会社	浓缩和纯化核酸的方法和装置
JP2005110503A (ja) *	2003-10-02	2005-04-28	Arkray Inc	核酸の精製方法及びデバイス
US20080035482A1 (en) *	2003-11-10	2008-02-14	Arkray Inc.	Method Of Concentrating And Purifying Nucleic Acid And Apparatus Therefor
KR100647306B1 (ko) *	2004-12-23	2006-11-23	삼성전자주식회사	아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
US20080085563A1 (en) *	2006-10-10	2008-04-10	Idexx Laboratories, Inc.	Detection of Gadolinium Chelates
US20080085562A1 (en) *	2006-10-10	2008-04-10	Idexx Laboratories, Inc.	Detection of gadolinium chelates
US20140027282A1 (en) *	2011-04-22	2014-01-30	Sony Corporation	Method of electrophoresing nucleic acids, method of concentrating and purifying nucleic acids, cartridge for nucleic acid electrophoresis, and method of producing cartridge for nucleic acid electrophoresis
EP2773221B1 (fr) *	2011-11-04	2019-01-09	Bio-Rad Laboratories, Inc.	Purification simultanée de composants cellulaires
US9766207B2 (en)	2011-11-04	2017-09-19	Bio-Rad Laboratories, Inc.	Affinity methods and compositions employing electronic control of pH
EP2814597A4 (fr)	2012-02-15	2015-10-28	Bio Rad Laboratories	Régulation électronique de ph et de force ionique
US9321012B2 (en)	2012-04-04	2016-04-26	Bio-Rad Laboratories, Inc.	Electronic protein fractionation

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4750982A (en)	1985-03-21	1988-06-14	Lifecodes Corp.	Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA
US4965188A (en)	1986-08-22	1990-10-23	Cetus Corporation	Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en)	1986-01-30	1987-07-28	Cetus Corporation	Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en)	1985-03-28	1987-07-28	Cetus Corporation	Process for amplifying nucleic acid sequences
US4822470A (en) *	1987-10-09	1989-04-18	Baylor College Of Medicine	Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
IE883226L (en)	1988-10-25	1990-04-25	Dna Prep Galway Ltd S 22	Separation of nucleic acids from protein material using¹cation exchangers
US5386024A (en)	1993-02-10	1995-01-31	Gen-Probe Incorporated	Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5635045A (en) *	1993-05-20	1997-06-03	Alam; Aftab	Apparatus for, and a method of, electroelution isolation of biomolecules and recovering biomolecules after elution
US5434270A (en)	1994-09-15	1995-07-18	Eastman Kodak Company	Weakly basic polymerizable monomers and polymers prepared therefrom
US5582988A (en)	1994-09-15	1996-12-10	Johnson & Johnson Clinical Diagnostics, Inc.	Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5973137A (en)	1996-02-13	1999-10-26	Gentra Systems, Inc.	Low pH RNA isolation reagents, method, and kit
DE19700364A1 (de) *	1997-01-08	1998-07-09	Bayer Ag	Elektrokinetische Probenvorbereitung

1999
- 1999-07-09 US US09/349,387 patent/US6699986B2/en not_active Expired - Fee Related
- 1999-07-29 CA CA2279131A patent/CA2279131C/fr not_active Expired - Fee Related
- 1999-08-03 AT AT99306146T patent/ATE449843T1/de not_active IP Right Cessation
- 1999-08-03 EP EP99306146A patent/EP0979868B1/fr not_active Expired - Lifetime
- 1999-08-03 DE DE69941655T patent/DE69941655D1/de not_active Expired - Lifetime
- 1999-08-04 JP JP25207299A patent/JP4531888B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
EP0979868A3 (fr)	2001-07-18
CA2279131A1 (fr)	2000-02-04
EP0979868A2 (fr)	2000-02-16
JP2000146911A (ja)	2000-05-26
ATE449843T1 (de)	2009-12-15
EP0979868B1 (fr)	2009-11-25
JP4531888B2 (ja)	2010-08-25
US20010049437A1 (en)	2001-12-06
DE69941655D1 (de)	2010-01-07
US6699986B2 (en)	2004-03-02

Legal Events

Date	Code	Title	Description
2003-12-12	EEER	Examination request
2013-09-10	MKLA	Lapsed	Effective date: 20130730

Publication	Publication Date	Title
US6914137B2 (en)	2005-07-05	Isolation of nucleic acids
CN1187362C (zh)	2005-02-02	核酸的固相分离法
EP0747388B1 (fr)	1999-09-22	Méthode et équipement pour la purification d'acides nucléiques
CA2279131C (fr)	2010-12-14	Separation electrophoretique des acides nucleiques des proteines a faible ph
US20150166592A1 (en)	2015-06-18	Selective Nucleic Acid Fragment Recovery
EP0969090A1 (fr)	2000-01-05	Procédé rapide et simple pour l'isolation d'acide nucleique circulaire
JPH09327291A (ja)	1997-12-22	Ｒｎａの抽出精製方法
JP3812696B2 (ja)	2006-08-23	リボ核酸の抽出方法
US20230203476A1 (en)	2023-06-29	Nucleic acid extraction method and application
WO2001062976A1 (fr)	2001-08-30	Methodes de separation, d'isolation et de purification rapides d'acide nucleique
JPH1075784A (ja)	1998-03-24	リボ核酸の単離方法
JPH11196869A (ja)	1999-07-27	リボ核酸の単離方法
JP3922420B2 (ja)	2007-05-30	改良されたリボ核酸の単離方法
KR100624447B1 (ko)	2006-09-18	은 나노 입자를 이용한 핵산의 정제 방법
KR100563621B1 (ko)	2006-03-23	인간 혈액으로부터의 유전체 ｄｎａ 추출용 키트 및이것을 이용한 유전체 ｄｎａ 추출 방법
JP3811767B2 (ja)	2006-08-23	均質な混合物から核酸を純化する方法
HK1034520B (en)	2006-03-24	Isolation of nucleic acids
HK1034520A1 (en)	2001-10-26	Isolation of nucleic acids