CA2279576A1 - Procede pour identifier le site d'action d'agents chimiques xenobiotiques - Google Patents
Procede pour identifier le site d'action d'agents chimiques xenobiotiques Download PDFInfo
- Publication number
- CA2279576A1 CA2279576A1 CA002279576A CA2279576A CA2279576A1 CA 2279576 A1 CA2279576 A1 CA 2279576A1 CA 002279576 A CA002279576 A CA 002279576A CA 2279576 A CA2279576 A CA 2279576A CA 2279576 A1 CA2279576 A1 CA 2279576A1
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- Prior art keywords
- detector
- bacteria
- gene
- stressor
- genotoxic
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Cette invention se rapporte à un procédé permettant de découvrir le site d'action de composés xénobiotiques. Ce procédé consiste à soumettre une cellule détecteur comprenant un promoteur sensible aux agents génotoxiques liés opérationnellement à un complexe de gènes reporteurs luminescents. L'exposition des bactéries détecteurs à des composés qui sont génotoxiques produit un accroîssement de la luminescence. Ces composés sont groupés et peuvent être analysés pour que leur activité pharmaceutique soit déterminée. L'exposition des bactéries détecteurs à des agents xénobiotiques actifs qui ne sont pas génotoxiques produit une inhibition de la bioluminescence ou de la luminescence stable. En soumettant les bactéries détecteurs à un tri, tel qu'un titrage génétique ou d'inversion nutritionnelle, on dévoile le site d'action du composé en question.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3958297P | 1997-02-28 | 1997-02-28 | |
| US60/039,582 | 1997-02-28 | ||
| PCT/US1998/003684 WO1998038336A1 (fr) | 1997-02-28 | 1998-02-26 | Procede pour identifier le site d'action d'agents chimiques xenobiotiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2279576A1 true CA2279576A1 (fr) | 1998-09-03 |
Family
ID=21906239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002279576A Abandoned CA2279576A1 (fr) | 1997-02-28 | 1998-02-26 | Procede pour identifier le site d'action d'agents chimiques xenobiotiques |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0972074A1 (fr) |
| JP (1) | JP2001514501A (fr) |
| AU (1) | AU6339098A (fr) |
| CA (1) | CA2279576A1 (fr) |
| IL (1) | IL131336A0 (fr) |
| WO (1) | WO1998038336A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT2308977E (pt) | 2004-04-30 | 2013-05-13 | Dow Agrosciences Llc | Novo gene de resistência a herbicida |
| DE102004026286B4 (de) * | 2004-05-28 | 2007-10-04 | Rene Girod Gmbh | Motorradständer |
| CN101553111B (zh) | 2005-10-28 | 2013-06-26 | 美国陶氏益农公司 | 新除草剂抗性基因 |
| JP2007312750A (ja) * | 2006-05-29 | 2007-12-06 | Daikin Ind Ltd | 改良された化学物質の検出方法 |
| JP2009039132A (ja) * | 2008-10-14 | 2009-02-26 | Daikin Ind Ltd | 改良された化学物質の検出方法 |
| SG11202112906TA (en) * | 2019-06-11 | 2021-12-30 | Fortephest Ltd | Novel non-coding heterocyclic amino acids (nchaa) and their use as herbicides |
| CN110241129B (zh) * | 2019-06-18 | 2022-11-15 | 山东大学 | 一种发光报告系统及其在抗生素发现筛选中的应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2117665T5 (es) * | 1991-03-01 | 2002-11-16 | Vito | Genes fusionados y su utilizacion para determinar la presencia de metales o de compuestos xenobioticos. |
| IL107815A (en) * | 1992-12-04 | 2003-12-10 | Du Pont | Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing |
| DK0680517T4 (da) * | 1993-01-21 | 2005-05-02 | Harvard College | Fremgangsmåde og diagnostiske sæt til bestemmelse af en forbindelses toksicitet under anvendelse afstress-promotorer fra pattedyr |
| DE69413491T2 (de) * | 1993-10-22 | 1999-03-11 | Kabushiki Kaisha Toyota Chuo Kenkyusho, Aichi | Verfahren zum Nachweis von Mutagenen unter Verwendung eines Lumineszensgens |
| DE69527850T2 (de) * | 1994-11-23 | 2003-04-24 | E.I. Du Pont De Nemours & Co., Inc. | Bioluminiscente lyophilisierte bakterien-reagenz zur giftstoff nachweit |
| DE19544374C1 (de) * | 1995-11-29 | 1997-02-20 | Dlr Deutsche Forschungsanstalt | Plasmid und damit transformierter Mikroorganismus für ein Verfahren zur luminometrischen Bestimmung der mutagenen und/oder karzinogenen Potenz eines Agens |
| WO1997041251A1 (fr) * | 1996-04-25 | 1997-11-06 | Vlaamse Instelling Voor Technologisch Onderzoek (Vito) | Sequences d'acides nucleiques recombinees, procede de determination a la fois de la genotoxicite et de la mutagenicite d'un echantillon, et cinetique de la genotoxicite |
| WO1998021347A1 (fr) * | 1996-11-15 | 1998-05-22 | E.I. Du Pont De Nemours And Company | Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement |
-
1998
- 1998-02-26 IL IL13133698A patent/IL131336A0/xx unknown
- 1998-02-26 AU AU63390/98A patent/AU6339098A/en not_active Abandoned
- 1998-02-26 WO PCT/US1998/003684 patent/WO1998038336A1/fr not_active Ceased
- 1998-02-26 EP EP98907633A patent/EP0972074A1/fr not_active Withdrawn
- 1998-02-26 CA CA002279576A patent/CA2279576A1/fr not_active Abandoned
- 1998-02-26 JP JP53781298A patent/JP2001514501A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP0972074A1 (fr) | 2000-01-19 |
| WO1998038336A1 (fr) | 1998-09-03 |
| JP2001514501A (ja) | 2001-09-11 |
| AU6339098A (en) | 1998-09-18 |
| IL131336A0 (en) | 2001-01-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |