CA2283094A1 - Detection de cibles au moyen d'une proteine fluorescente verte et de ses variantes fluorescentes - Google Patents

Detection de cibles au moyen d'une proteine fluorescente verte et de ses variantes fluorescentes Download PDF

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Publication number
CA2283094A1
CA2283094A1 CA002283094A CA2283094A CA2283094A1 CA 2283094 A1 CA2283094 A1 CA 2283094A1 CA 002283094 A CA002283094 A CA 002283094A CA 2283094 A CA2283094 A CA 2283094A CA 2283094 A1 CA2283094 A1 CA 2283094A1
Authority
CA
Canada
Prior art keywords
ligand
group
biotin
avidin
label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002283094A
Other languages
English (en)
Inventor
Todd W. Plaia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Oncor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncor Inc filed Critical Oncor Inc
Publication of CA2283094A1 publication Critical patent/CA2283094A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
    • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
    • C07D307/04—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
    • C07D307/10—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having no double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D307/16—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
    • C07D309/08—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D309/14—Nitrogen atoms not forming part of a nitro radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D309/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
    • C07D309/16—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D309/28—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/14—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)

Abstract

On combine une protéine fluorescente verte ou ses variantes fluorescentes avec des ligands afin de les utiliser en tant que marqueurs fluorescents dans la détection de cibles. Les ligands appropriés comprennent des sondes d'acide nucléique, des anticorps, des conjugués d'haptènes, biotine, avidine et streptavidine. On met en application des techniques, telles que la microscopie fluorescente, afin visualiser le marqueur.
CA002283094A 1997-02-18 1998-02-18 Detection de cibles au moyen d'une proteine fluorescente verte et de ses variantes fluorescentes Abandoned CA2283094A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3862397P 1997-02-18 1997-02-18
US60/038,623 1997-02-18
PCT/US1998/003147 WO1998036099A1 (fr) 1997-02-18 1998-02-18 Detection de cibles au moyen d'une proteine fluorescente verte et de ses variantes fluorescentes

Publications (1)

Publication Number Publication Date
CA2283094A1 true CA2283094A1 (fr) 1998-08-20

Family

ID=21900962

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002283094A Abandoned CA2283094A1 (fr) 1997-02-18 1998-02-18 Detection de cibles au moyen d'une proteine fluorescente verte et de ses variantes fluorescentes

Country Status (5)

Country Link
EP (1) EP1000172A4 (fr)
JP (1) JP2001514741A (fr)
AU (1) AU6658898A (fr)
CA (1) CA2283094A1 (fr)
WO (1) WO1998036099A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6495664B1 (en) 1998-07-24 2002-12-17 Aurora Biosciences Corporation Fluorescent protein sensors of post-translational modifications
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
CA2445420A1 (fr) 2003-07-29 2005-01-29 Invitrogen Corporation Tests de kinase et de phosphatase
DE602005024673D1 (de) 2004-04-08 2010-12-23 Roche Diagnostics Gmbh Fluoreszenzpolypeptidkomplex einer grösse von 40 bis 500 nm
US20130085095A1 (en) * 2011-09-29 2013-04-04 Trustees Of Boston University Protein complementation regulators
DE102014005112A1 (de) 2014-05-22 2015-11-26 Emschergenossenschaft Messung unterirdischer Bauwerke, insbesonder beim unterirdischen Vortrieb, mit Prismen

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469797A (en) * 1982-09-23 1984-09-04 Miles Laboratories, Inc. Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives
US5084378A (en) * 1987-11-30 1992-01-28 The United States Of America As Represented By The United States Department Of Energy Enhanced detection of fluorescence quenching in labeled cells
WO1994018342A1 (fr) * 1993-02-12 1994-08-18 Sealite Sciences, Inc. Preparation de conjugues de photoproteines et leur procede d'utilisation
AU2253397A (en) * 1996-01-23 1997-08-20 Affymetrix, Inc. Nucleic acid analysis techniques

Also Published As

Publication number Publication date
EP1000172A1 (fr) 2000-05-17
EP1000172A4 (fr) 2002-10-30
AU6658898A (en) 1998-09-08
JP2001514741A (ja) 2001-09-11
WO1998036099A1 (fr) 1998-08-20

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Legal Events

Date Code Title Description
FZDE Discontinued