CA2295931C - Procede d'identification d'une variete d'orge et orge presentant une propriete brassicole - Google Patents
Procede d'identification d'une variete d'orge et orge presentant une propriete brassicole Download PDFInfo
- Publication number
- CA2295931C CA2295931C CA2295931A CA2295931A CA2295931C CA 2295931 C CA2295931 C CA 2295931C CA 2295931 A CA2295931 A CA 2295931A CA 2295931 A CA2295931 A CA 2295931A CA 2295931 C CA2295931 C CA 2295931C
- Authority
- CA
- Canada
- Prior art keywords
- amylase
- beta
- barley
- thermostability
- variety
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000209219 Hordeum Species 0.000 title claims abstract description 109
- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 109
- 238000000034 method Methods 0.000 title claims abstract description 99
- 239000004382 Amylase Substances 0.000 claims abstract description 104
- 230000000694 effects Effects 0.000 claims abstract description 33
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 19
- 239000000243 solution Substances 0.000 claims description 45
- 238000001962 electrophoresis Methods 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 238000001155 isoelectric focusing Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 239000008055 phosphate buffer solution Substances 0.000 claims description 3
- 108091008146 restriction endonucleases Proteins 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 125000004201 2,4-dichlorophenyl group Chemical group [H]C1=C([H])C(*)=C(Cl)C([H])=C1Cl 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract description 11
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 238000010187 selection method Methods 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 31
- 230000001488 breeding effect Effects 0.000 description 29
- 235000013405 beer Nutrition 0.000 description 10
- 238000010438 heat treatment Methods 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 6
- 238000004890 malting Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HFZWRUODUSTPEG-UHFFFAOYSA-N 2,4-dichlorophenol Chemical compound OC1=CC=C(Cl)C=C1Cl HFZWRUODUSTPEG-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000241985 Cnides Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 239000006173 Good's buffer Substances 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- BKHZIBWEHPHYAI-UHFFFAOYSA-N chloroform;3-methylbutan-1-ol Chemical compound ClC(Cl)Cl.CC(C)CCO BKHZIBWEHPHYAI-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2422—Alpha-amylase (3.2.1.1.) from plant source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/926—Hydrolases (3) acting on glycosyl compounds (3.2) acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Physiology (AREA)
- Hematology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Procédé d'identification d'orge possédant de bonnes propriétés brassicoles, sur la base de la thermostabilité de la .beta.-amylase de l'orge en tant qu'indicateur. La thermostabilité de la .beta.-amylase de l'orge influence considérablement la limite d'atténuation apparente. Un procédé permettant de déterminer l'activité enzymatique d'une solution extraite de graine d'orge, un procédé indirect basé sur le point isoélectrique et un procédé d'identification indirecte par polymorphismes de l'ADN de la région contenant le gène structural de .beta.-amylase ont été mis au point en tant que procédé permettant de déterminer le type de thermostabilité d'une .beta.-amylase d'orge. Ledit procédé de sélection n'est pas influencé par des conditions relatives au climat ou à l'environnement.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9/170582 | 1997-06-26 | ||
| JP17058297 | 1997-06-26 | ||
| PCT/JP1998/002814 WO1999000514A1 (fr) | 1997-06-26 | 1998-06-24 | Procede d'identification d'une variete d'orge et orge presentant une propriete brassicole |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2295931A1 CA2295931A1 (fr) | 1999-01-07 |
| CA2295931C true CA2295931C (fr) | 2010-05-18 |
Family
ID=15907516
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2295931A Expired - Fee Related CA2295931C (fr) | 1997-06-26 | 1998-06-24 | Procede d'identification d'une variete d'orge et orge presentant une propriete brassicole |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6492576B2 (fr) |
| EP (1) | EP0990043B2 (fr) |
| JP (2) | JP4824843B2 (fr) |
| AU (1) | AU746726B2 (fr) |
| CA (1) | CA2295931C (fr) |
| WO (1) | WO1999000514A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002335181B2 (en) * | 2001-10-04 | 2007-04-05 | Sapporo Breweries Limited | Method of selecting barley variety, barley beta-amylase gene and process for producing malt alcoholic drink |
| AU2004220052B2 (en) | 2003-03-10 | 2010-08-19 | Genencor International Inc. | Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same |
| JP2011139669A (ja) * | 2010-01-07 | 2011-07-21 | Sapporo Breweries Ltd | 大麦の選別方法、麦芽及び麦芽発酵飲料 |
| CN110095329B (zh) * | 2019-06-06 | 2021-04-30 | 山东农业大学 | 一种快速测定小麦种子纯度的染色方法 |
| CN119391675A (zh) * | 2024-10-25 | 2025-02-07 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | β-淀粉酶热稳定性调控方法及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL9000997A (nl) * | 1990-04-26 | 1991-11-18 | Royal Sluis Koninkl Zaaizaadbe | Werkwijze voor het bepalen van de hybride kwaliteit van zaden. |
| EP0802720A4 (fr) * | 1994-06-21 | 1999-01-13 | Zeneca Ltd | Nouvelles plantes et leur procede d'obtention |
| JP3471916B2 (ja) * | 1994-09-28 | 2003-12-02 | サッポロホールディングス株式会社 | 組換えβ−アミラーゼ |
| JP3660699B2 (ja) * | 1994-09-29 | 2005-06-15 | サッポロビール株式会社 | 遺伝子診断を用いた大麦又は麦芽の品種識別方法及びそのプライマー |
| JP4424756B2 (ja) | 1995-07-05 | 2010-03-03 | サッポロビール株式会社 | 組織特異的プロモーターを用いたトランスジェニック植物およびその作出方法 |
-
1998
- 1998-06-24 CA CA2295931A patent/CA2295931C/fr not_active Expired - Fee Related
- 1998-06-24 AU AU79329/98A patent/AU746726B2/en not_active Expired
- 1998-06-24 JP JP50542399A patent/JP4824843B2/ja not_active Expired - Lifetime
- 1998-06-24 EP EP98929662A patent/EP0990043B2/fr not_active Expired - Lifetime
- 1998-06-24 WO PCT/JP1998/002814 patent/WO1999000514A1/fr not_active Ceased
- 1998-06-26 US US09/104,536 patent/US6492576B2/en not_active Expired - Lifetime
-
2008
- 2008-09-29 JP JP2008251780A patent/JP4450856B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6492576B2 (en) | 2002-12-10 |
| AU746726B2 (en) | 2002-05-02 |
| JP2009060911A (ja) | 2009-03-26 |
| JP2002508667A (ja) | 2002-03-19 |
| CA2295931A1 (fr) | 1999-01-07 |
| EP0990043A1 (fr) | 2000-04-05 |
| WO1999000514A1 (fr) | 1999-01-07 |
| JP4450856B2 (ja) | 2010-04-14 |
| AU7932998A (en) | 1999-01-19 |
| EP0990043B1 (fr) | 2007-01-17 |
| JP4824843B2 (ja) | 2011-11-30 |
| US20020019994A1 (en) | 2002-02-14 |
| EP0990043B2 (fr) | 2012-07-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20180626 |