CA2302861A1 - Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries - Google Patents

Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries Download PDF

Info

Publication number: CA2302861A1
Authority: CA; Canada
Prior art keywords: nucleic acid; csp; acid molecule; peptide; seq
Prior art date: 2000-04-10
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2302861

Other languages

English (en)

Inventor

Dennis G. Cvitkovitch

Peter C. Y. Lau

Yung Hua Li

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of Toronto

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-04-10

Filing date

2000-04-10

Publication date

2001-10-10

2000-04-10 Application filed by Individual filed Critical Individual

2000-04-10 Priority to CA 2302861 priority Critical patent/CA2302861A1/fr

2001-02-20 Priority to CA2332733A priority patent/CA2332733C/fr

2001-04-10 Priority to US09/833,017 priority patent/US6923962B2/en

2001-10-10 Publication of CA2302861A1 publication Critical patent/CA2302861A1/fr

Status Abandoned legal-status Critical Current

Links

150000007523 nucleic acids Chemical class 0.000 title claims description 123
108020004707 nucleic acids Proteins 0.000 title claims description 122
102000039446 nucleic acids Human genes 0.000 title claims description 122
238000000034 method Methods 0.000 title claims description 60
108010076504 Protein Sorting Signals Proteins 0.000 title claims description 23
208000002925 dental caries Diseases 0.000 title claims description 22
238000011282 treatment Methods 0.000 title claims description 7
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 188
241000194019 Streptococcus mutans Species 0.000 claims abstract description 53
108010072039 Histidine kinase Proteins 0.000 claims abstract description 52
150000001875 compounds Chemical class 0.000 claims abstract description 45
239000012634 fragment Substances 0.000 claims abstract description 31
210000004027 cell Anatomy 0.000 claims description 79
102000004196 processed proteins & peptides Human genes 0.000 claims description 77
230000000694 effects Effects 0.000 claims description 56
108020004414 DNA Proteins 0.000 claims description 41
108090000623 proteins and genes Proteins 0.000 claims description 40
229920001184 polypeptide Polymers 0.000 claims description 29
230000002068 genetic effect Effects 0.000 claims description 25
230000009466 transformation Effects 0.000 claims description 22
150000001413 amino acids Chemical class 0.000 claims description 21
239000013598 vector Substances 0.000 claims description 21
239000002773 nucleotide Substances 0.000 claims description 20
125000003729 nucleotide group Chemical group 0.000 claims description 20
229960005486 vaccine Drugs 0.000 claims description 20
239000000203 mixture Substances 0.000 claims description 17
230000014509 gene expression Effects 0.000 claims description 15
101710082261 Competence-stimulating peptide Proteins 0.000 claims description 14
239000002253 acid Substances 0.000 claims description 14
230000032770 biofilm formation Effects 0.000 claims description 13
239000008194 pharmaceutical composition Substances 0.000 claims description 9
238000012258 culturing Methods 0.000 claims description 6
230000001580 bacterial effect Effects 0.000 claims description 5
239000002299 complementary DNA Substances 0.000 claims description 5
238000009396 hybridization Methods 0.000 claims description 5
230000000295 complement effect Effects 0.000 claims description 4
206010014665 endocarditis Diseases 0.000 claims description 4
230000001939 inductive effect Effects 0.000 claims description 4
238000004519 manufacturing process Methods 0.000 claims description 4
230000002829 reductive effect Effects 0.000 claims description 3
108010058846 Ovalbumin Proteins 0.000 claims description 2
102000009843 Thyroglobulin Human genes 0.000 claims description 2
108010034949 Thyroglobulin Proteins 0.000 claims description 2
230000002538 fungal effect Effects 0.000 claims description 2
239000003550 marker Substances 0.000 claims description 2
229940092253 ovalbumin Drugs 0.000 claims description 2
238000011321 prophylaxis Methods 0.000 claims description 2
229960002175 thyroglobulin Drugs 0.000 claims description 2
238000013518 transcription Methods 0.000 claims description 2
230000035897 transcription Effects 0.000 claims description 2
108091033380 Coding strand Proteins 0.000 claims 1
244000005700 microbiome Species 0.000 claims 1
210000005253 yeast cell Anatomy 0.000 claims 1
239000003112 inhibitor Substances 0.000 description 27
229940024606 amino acid Drugs 0.000 description 24
238000003556 assay Methods 0.000 description 24
230000004044 response Effects 0.000 description 24
235000001014 amino acid Nutrition 0.000 description 20
102000001253 Protein Kinase Human genes 0.000 description 16
238000011534 incubation Methods 0.000 description 15
239000013612 plasmid Substances 0.000 description 15
102000004169 proteins and genes Human genes 0.000 description 14
206010010071 Coma Diseases 0.000 description 13
ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 13
239000002609 medium Substances 0.000 description 13
125000003275 alpha amino acid group Chemical group 0.000 description 12
235000018102 proteins Nutrition 0.000 description 12
239000000523 sample Substances 0.000 description 11
238000012360 testing method Methods 0.000 description 11
241000894006 Bacteria Species 0.000 description 10
230000015572 biosynthetic process Effects 0.000 description 9
239000013611 chromosomal DNA Substances 0.000 description 8
238000003752 polymerase chain reaction Methods 0.000 description 8
238000007792 addition Methods 0.000 description 7
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
230000035772 mutation Effects 0.000 description 7
238000006467 substitution reaction Methods 0.000 description 7
108091028043 Nucleic acid sequence Proteins 0.000 description 6
239000000872 buffer Substances 0.000 description 6
229960003276 erythromycin Drugs 0.000 description 6
239000012530 fluid Substances 0.000 description 6
238000002360 preparation method Methods 0.000 description 6
230000008569 process Effects 0.000 description 6
230000001105 regulatory effect Effects 0.000 description 6
239000003981 vehicle Substances 0.000 description 6
108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
230000004913 activation Effects 0.000 description 5
239000003242 anti bacterial agent Substances 0.000 description 5
229940088710 antibiotic agent Drugs 0.000 description 5
230000003115 biocidal effect Effects 0.000 description 5
239000003795 chemical substances by application Substances 0.000 description 5
201000010099 disease Diseases 0.000 description 5
239000013604 expression vector Substances 0.000 description 5
230000004927 fusion Effects 0.000 description 5
230000001900 immune effect Effects 0.000 description 5
230000001965 increasing effect Effects 0.000 description 5
230000003993 interaction Effects 0.000 description 5
230000001404 mediated effect Effects 0.000 description 5
239000000126 substance Substances 0.000 description 5
229920001817 Agar Polymers 0.000 description 4
108091026890 Coding region Proteins 0.000 description 4
102000009338 Gastric Mucins Human genes 0.000 description 4
108010009066 Gastric Mucins Proteins 0.000 description 4
241000282414 Homo sapiens Species 0.000 description 4
241000124008 Mammalia Species 0.000 description 4
241001465754 Metazoa Species 0.000 description 4
PQMWYJDJHJQZDE-UHFFFAOYSA-M Methantheline bromide Chemical compound [Br-].C1=CC=C2C(C(=O)OCC[N+](C)(CC)CC)C3=CC=CC=C3OC2=C1 PQMWYJDJHJQZDE-UHFFFAOYSA-M 0.000 description 4
239000004793 Polystyrene Substances 0.000 description 4
230000002378 acidificating effect Effects 0.000 description 4
239000008272 agar Substances 0.000 description 4
101150103565 comX gene Proteins 0.000 description 4
230000001276 controlling effect Effects 0.000 description 4
238000012217 deletion Methods 0.000 description 4
230000037430 deletion Effects 0.000 description 4
230000003053 immunization Effects 0.000 description 4
238000002649 immunization Methods 0.000 description 4
238000000338 in vitro Methods 0.000 description 4
239000000463 material Substances 0.000 description 4
230000000813 microbial effect Effects 0.000 description 4
239000000546 pharmaceutical excipient Substances 0.000 description 4
229920002223 polystyrene Polymers 0.000 description 4
239000013615 primer Substances 0.000 description 4
210000002966 serum Anatomy 0.000 description 4
108010043121 Green Fluorescent Proteins Proteins 0.000 description 3
102000004144 Green Fluorescent Proteins Human genes 0.000 description 3
241000282412 Homo Species 0.000 description 3
238000009825 accumulation Methods 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
239000005557 antagonist Substances 0.000 description 3
230000000692 anti-sense effect Effects 0.000 description 3
239000012472 biological sample Substances 0.000 description 3
229940041514 candida albicans extract Drugs 0.000 description 3
239000003153 chemical reaction reagent Substances 0.000 description 3
101150100845 comC gene Proteins 0.000 description 3
238000001514 detection method Methods 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
239000012737 fresh medium Substances 0.000 description 3
239000011521 glass Substances 0.000 description 3
239000005090 green fluorescent protein Substances 0.000 description 3
125000001165 hydrophobic group Chemical group 0.000 description 3
230000036039 immunity Effects 0.000 description 3
230000005847 immunogenicity Effects 0.000 description 3
230000002779 inactivation Effects 0.000 description 3
230000006698 induction Effects 0.000 description 3
208000015181 infectious disease Diseases 0.000 description 3
230000037431 insertion Effects 0.000 description 3
238000003780 insertion Methods 0.000 description 3
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
239000002502 liposome Substances 0.000 description 3
239000002324 mouth wash Substances 0.000 description 3
239000002243 precursor Substances 0.000 description 3
102000005962 receptors Human genes 0.000 description 3
108020003175 receptors Proteins 0.000 description 3
238000011160 research Methods 0.000 description 3
238000000527 sonication Methods 0.000 description 3
230000004936 stimulating effect Effects 0.000 description 3
239000000606 toothpaste Substances 0.000 description 3
239000012138 yeast extract Substances 0.000 description 3
241000271566 Aves Species 0.000 description 2
208000035143 Bacterial infection Diseases 0.000 description 2
108020004635 Complementary DNA Proteins 0.000 description 2
208000002064 Dental Plaque Diseases 0.000 description 2
241000192125 Firmicutes Species 0.000 description 2
241000628997 Flos Species 0.000 description 2
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
241000238631 Hexapoda Species 0.000 description 2
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
108020005187 Oligonucleotide Probes Proteins 0.000 description 2
206010073261 Ovarian theca cell tumour Diseases 0.000 description 2
208000037581 Persistent Infection Diseases 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
241000700605 Viruses Species 0.000 description 2
230000002159 abnormal effect Effects 0.000 description 2
230000009471 action Effects 0.000 description 2
239000000654 additive Substances 0.000 description 2
125000000539 amino acid group Chemical group 0.000 description 2
230000000845 anti-microbial effect Effects 0.000 description 2
239000000427 antigen Substances 0.000 description 2
102000036639 antigens Human genes 0.000 description 2
108091007433 antigens Proteins 0.000 description 2
239000003963 antioxidant agent Substances 0.000 description 2
235000003704 aspartic acid Nutrition 0.000 description 2
208000022362 bacterial infectious disease Diseases 0.000 description 2
230000008901 benefit Effects 0.000 description 2
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
239000000969 carrier Substances 0.000 description 2
238000004113 cell culture Methods 0.000 description 2
239000006285 cell suspension Substances 0.000 description 2
229940112822 chewing gum Drugs 0.000 description 2
235000015218 chewing gum Nutrition 0.000 description 2
238000003776 cleavage reaction Methods 0.000 description 2
238000000975 co-precipitation Methods 0.000 description 2
230000002860 competitive effect Effects 0.000 description 2
230000006957 competitive inhibition Effects 0.000 description 2
230000008878 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
230000002950 deficient Effects 0.000 description 2
238000013461 design Methods 0.000 description 2
238000011161 development Methods 0.000 description 2
239000003085 diluting agent Substances 0.000 description 2
208000035475 disorder Diseases 0.000 description 2
229940079593 drug Drugs 0.000 description 2
239000003814 drug Substances 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
239000003623 enhancer Substances 0.000 description 2
239000000499 gel Substances 0.000 description 2
235000013922 glutamic acid Nutrition 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
230000028996 humoral immune response Effects 0.000 description 2
230000028993 immune response Effects 0.000 description 2
230000002163 immunogen Effects 0.000 description 2
-1 inhalants Substances 0.000 description 2
230000002401 inhibitory effect Effects 0.000 description 2
230000005764 inhibitory process Effects 0.000 description 2
229930027917 kanamycin Natural products 0.000 description 2
229960000318 kanamycin Drugs 0.000 description 2
229930182823 kanamycin A Natural products 0.000 description 2
238000005259 measurement Methods 0.000 description 2
238000000520 microinjection Methods 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000010369 molecular cloning Methods 0.000 description 2
238000012544 monitoring process Methods 0.000 description 2
229940051866 mouthwash Drugs 0.000 description 2
238000002703 mutagenesis Methods 0.000 description 2
231100000350 mutagenesis Toxicity 0.000 description 2
239000002751 oligonucleotide probe Substances 0.000 description 2
229940126578 oral vaccine Drugs 0.000 description 2
230000036961 partial effect Effects 0.000 description 2
230000035790 physiological processes and functions Effects 0.000 description 2
238000011002 quantification Methods 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
230000007017 scission Effects 0.000 description 2
238000012216 screening Methods 0.000 description 2
238000012163 sequencing technique Methods 0.000 description 2
238000002741 site-directed mutagenesis Methods 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000007787 solid Substances 0.000 description 2
230000000638 stimulation Effects 0.000 description 2
239000000375 suspending agent Substances 0.000 description 2
238000003786 synthesis reaction Methods 0.000 description 2
208000001644 thecoma Diseases 0.000 description 2
210000001519 tissue Anatomy 0.000 description 2
229940034610 toothpaste Drugs 0.000 description 2
230000000699 topical effect Effects 0.000 description 2
230000002103 transcriptional effect Effects 0.000 description 2
238000012546 transfer Methods 0.000 description 2
238000011144 upstream manufacturing Methods 0.000 description 2
238000005406 washing Methods 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
OZDAOHVKBFBBMZ-UHFFFAOYSA-N 2-aminopentanedioic acid;hydrate Chemical compound O.OC(=O)C(N)CCC(O)=O OZDAOHVKBFBBMZ-UHFFFAOYSA-N 0.000 description 1
241000589155 Agrobacterium tumefaciens Species 0.000 description 1
108020005544 Antisense RNA Proteins 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
101100440622 Bacillus subtilis (strain 168) yuxO gene Proteins 0.000 description 1
108010001857 Cell Surface Receptors Proteins 0.000 description 1
102000009016 Cholera Toxin Human genes 0.000 description 1
108010049048 Cholera Toxin Proteins 0.000 description 1
108020004705 Codon Proteins 0.000 description 1
150000008574 D-amino acids Chemical class 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
241000702421 Dependoparvovirus Species 0.000 description 1
241000196324 Embryophyta Species 0.000 description 1
108091060211 Expressed sequence tag Proteins 0.000 description 1
241000233866 Fungi Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
102000000340 Glucosyltransferases Human genes 0.000 description 1
108010055629 Glucosyltransferases Proteins 0.000 description 1
101001005708 Homo sapiens MARVEL domain-containing protein 1 Proteins 0.000 description 1
102100034343 Integrase Human genes 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
102100025069 MARVEL domain-containing protein 1 Human genes 0.000 description 1
241000699666 Mus <mouse, genus> Species 0.000 description 1
241000699670 Mus sp. Species 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
238000012408 PCR amplification Methods 0.000 description 1
108010067902 Peptide Library Proteins 0.000 description 1
101800001442 Peptide pr Proteins 0.000 description 1
239000004743 Polypropylene Substances 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
206010057190 Respiratory tract infections Diseases 0.000 description 1
238000012300 Sequence Analysis Methods 0.000 description 1
108010071390 Serum Albumin Proteins 0.000 description 1
102000007562 Serum Albumin Human genes 0.000 description 1
206010061372 Streptococcal infection Diseases 0.000 description 1
241000193998 Streptococcus pneumoniae Species 0.000 description 1
108010008038 Synthetic Vaccines Proteins 0.000 description 1
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
239000012190 activator Substances 0.000 description 1
239000002671 adjuvant Substances 0.000 description 1
230000002776 aggregation Effects 0.000 description 1
238000004220 aggregation Methods 0.000 description 1
239000000556 agonist Substances 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
230000006229 amino acid addition Effects 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
230000003321 amplification Effects 0.000 description 1
230000003042 antagnostic effect Effects 0.000 description 1
230000000844 anti-bacterial effect Effects 0.000 description 1
230000003078 antioxidant effect Effects 0.000 description 1
238000013459 approach Methods 0.000 description 1
238000009360 aquaculture Methods 0.000 description 1
244000144974 aquaculture Species 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
235000013361 beverage Nutrition 0.000 description 1
101150114605 bhsA gene Proteins 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
239000007474 bm medium Substances 0.000 description 1
239000008366 buffered solution Substances 0.000 description 1
238000010804 cDNA synthesis Methods 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
210000000170 cell membrane Anatomy 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
101150109808 comC1 gene Proteins 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000001816 cooling Methods 0.000 description 1
239000000287 crude extract Substances 0.000 description 1
GICLSALZHXCILJ-UHFFFAOYSA-N ctk5a5089 Chemical group NCC(O)=O.NCC(O)=O GICLSALZHXCILJ-UHFFFAOYSA-N 0.000 description 1
238000011461 current therapy Methods 0.000 description 1
230000006735 deficit Effects 0.000 description 1
210000003298 dental enamel Anatomy 0.000 description 1
239000008121 dextrose Substances 0.000 description 1
235000014113 dietary fatty acids Nutrition 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
239000000975 dye Substances 0.000 description 1
238000004520 electroporation Methods 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
239000003995 emulsifying agent Substances 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
229930195729 fatty acid Natural products 0.000 description 1
239000000194 fatty acid Substances 0.000 description 1
150000004665 fatty acids Chemical class 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
239000000945 filler Substances 0.000 description 1
239000000796 flavoring agent Substances 0.000 description 1
235000019634 flavors Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
230000005714 functional activity Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
238000007511 glassblowing Methods 0.000 description 1
108090001082 glucan-binding proteins Proteins 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
101150031572 gtfA gene Proteins 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
125000004356 hydroxy functional group Chemical group O* 0.000 description 1
210000000987 immune system Anatomy 0.000 description 1
239000007943 implant Substances 0.000 description 1
238000000099 in vitro assay Methods 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
201000007119 infective endocarditis Diseases 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002347 injection Methods 0.000 description 1
230000010354 integration Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
230000002147 killing effect Effects 0.000 description 1
239000008101 lactose Substances 0.000 description 1
230000021633 leukocyte mediated immunity Effects 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000009630 liquid culture Methods 0.000 description 1
239000012669 liquid formulation Substances 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
239000011159 matrix material Substances 0.000 description 1
238000011089 mechanical engineering Methods 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
102000006240 membrane receptors Human genes 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
108091005601 modified peptides Proteins 0.000 description 1
125000000896 monocarboxylic acid group Chemical group 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
238000001139 pH measurement Methods 0.000 description 1
238000007911 parenteral administration Methods 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
229940023041 peptide vaccine Drugs 0.000 description 1
235000020030 perry Nutrition 0.000 description 1
230000002688 persistence Effects 0.000 description 1
230000002085 persistent effect Effects 0.000 description 1
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
150000003904 phospholipids Chemical class 0.000 description 1
238000000053 physical method Methods 0.000 description 1
230000035479 physiological effects, processes and functions Effects 0.000 description 1
239000013600 plasmid vector Substances 0.000 description 1
238000007747 plating Methods 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
230000003389 potentiating effect Effects 0.000 description 1
239000003755 preservative agent Substances 0.000 description 1
230000002335 preservative effect Effects 0.000 description 1
230000002265 prevention Effects 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
201000007094 prostatitis Diseases 0.000 description 1
230000001681 protective effect Effects 0.000 description 1
230000004952 protein activity Effects 0.000 description 1
230000017854 proteolysis Effects 0.000 description 1
238000005086 pumping Methods 0.000 description 1
238000000746 purification Methods 0.000 description 1
230000018612 quorum sensing Effects 0.000 description 1
229940124551 recombinant vaccine Drugs 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
230000001177 retroviral effect Effects 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
238000005070 sampling Methods 0.000 description 1
230000003248 secreting effect Effects 0.000 description 1
238000002864 sequence alignment Methods 0.000 description 1
239000002689 soil Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
241000894007 species Species 0.000 description 1
239000007921 spray Substances 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
229940031000 streptococcus pneumoniae Drugs 0.000 description 1
239000000725 suspension Substances 0.000 description 1
239000003826 tablet Substances 0.000 description 1
230000008685 targeting Effects 0.000 description 1
230000001225 therapeutic effect Effects 0.000 description 1
239000002562 thickening agent Substances 0.000 description 1
238000011200 topical administration Methods 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
238000001890 transfection Methods 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
239000004474 valine Substances 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
238000002424 x-ray crystallography Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)

CA 2302861 2000-04-10 2000-04-10 Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries Abandoned CA2302861A1 (fr)

Priority Applications (3)

Application Number	Priority Date	Filing Date	Title
CA 2302861 CA2302861A1 (fr)	2000-04-10	2000-04-10	Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries
CA2332733A CA2332733C (fr)	2000-04-10	2001-02-20	Sequences-signal, molecules d'acide nucleique et methode de traitement des caries
US09/833,017 US6923962B2 (en)	2000-04-10	2001-04-10	Signal peptides, nucleic acid molecules and methods for treatment of caries

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA 2302861 CA2302861A1 (fr)	2000-04-10	2000-04-10	Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries

Publications (1)

Publication Number	Publication Date
CA2302861A1 true CA2302861A1 (fr)	2001-10-10

Family

ID=4165684

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2302861 Abandoned CA2302861A1 (fr)	2000-04-10	2000-04-10	Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries

Country Status (1)

Country	Link
CA (1)	CA2302861A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2008074473A2 (fr)	2006-12-19	2008-06-26	Basf Se	Utilisations et procédés de prévention et/ou de traitement de caries provoquées par des streptocoques mutants
EP2218774A2 (fr)	2004-09-10	2010-08-18	Basf Se	Méthodes et moyens à prevenir et/ou traiter des caries
CN112646903A (zh) *	2020-12-28	2021-04-13	遵义医科大学珠海校区	肺炎链球菌对万古霉素耐药性诊断的标志物及其应用

2000
- 2000-04-10 CA CA 2302861 patent/CA2302861A1/fr not_active Abandoned

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP2218774A2 (fr)	2004-09-10	2010-08-18	Basf Se	Méthodes et moyens à prevenir et/ou traiter des caries
EP2267112A1 (fr)	2004-09-10	2010-12-29	Basf Se	Méthodes et moyens à prevenir et/ou traiter des caries
WO2008074473A2 (fr)	2006-12-19	2008-06-26	Basf Se	Utilisations et procédés de prévention et/ou de traitement de caries provoquées par des streptocoques mutants
CN112646903A (zh) *	2020-12-28	2021-04-13	遵义医科大学珠海校区	肺炎链球菌对万古霉素耐药性诊断的标志物及其应用

Legal Events

Date	Code	Title	Description
2005-04-08	EEER	Examination request
2009-11-16	FZDE	Dead

Publication	Publication Date	Title
US20100016234A1 (en)	2010-01-21	Signal peptides, nucleic acid molecules and methods for treatment of caries
US20080241151A1 (en)	2008-10-02	Virulence genes, proteins, and their use
EP0871733A2 (fr)	1998-10-21	Genes clones de porphyromonas gingivalis et sondes de detection de paradontopathie
TWI328035B (en)	2010-08-01	Anti-bacterial vaccine compositions
EP2281834B1 (fr)	2015-03-04	Bactéries atténuées de la Famille Pasteurellaceae comprenant un gène muté de virulence
CA2481186C (fr)	2015-10-06	Bacteries gram negatives attenuees
US6923962B2 (en)	2005-08-02	Signal peptides, nucleic acid molecules and methods for treatment of caries
EP1330473A2 (fr)	2003-07-30	Genes streptococciques
KR100391483B1 (ko)	2003-10-30	비유형 헤모필러스 인플루엔재(Haemophilus influenzae)의 LKP필린 구조유전자 및 오페론
US8313749B2 (en)	2012-11-20	P. gingivalis vaccine
JP4932731B2 (ja)	2012-05-16	シグナルペプチド、核酸分子及び治療方法
CA2332733C (fr)	2012-10-02	Sequences-signal, molecules d'acide nucleique et methode de traitement des caries
US20040171020A1 (en)	2004-09-02	Glanders/meliodosis vaccines
CA2302861A1 (fr)	2001-10-10	Molecules d'acide nucleique codant pour des sequences-signal et methode de traitement des caries
EP1905834A2 (fr)	2008-04-02	Gènes de virulence, protéines et leur utilisation
US7556807B2 (en)	2009-07-07	Signal peptides, nucleic acid molecules and methods for treatment of caries
KR20080052693A (ko)	2008-06-11	유전자, 단백질 및 그들의 용도
JP4183504B2 (ja)	2008-11-19	ストレプトコッカス遺伝子
WO1996040926A2 (fr)	1996-12-19	Detection de cryptosporicium parvum
WO2002077020A2 (fr)	2002-10-03	Genes de virulence dans h. influenzae