CA2303463A1 - Sondes d'hybridation destinees a differencier les streptomycetes des maduromycetes - Google Patents

Sondes d'hybridation destinees a differencier les streptomycetes des maduromycetes Download PDF

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Publication number
CA2303463A1
CA2303463A1 CA002303463A CA2303463A CA2303463A1 CA 2303463 A1 CA2303463 A1 CA 2303463A1 CA 002303463 A CA002303463 A CA 002303463A CA 2303463 A CA2303463 A CA 2303463A CA 2303463 A1 CA2303463 A1 CA 2303463A1
Authority
CA
Canada
Prior art keywords
probe
maduromycetes
rrna
nucleic acids
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002303463A
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English (en)
Inventor
Olga Genilloud
Rafael P. Mellado
Victor Parro
Vicente Rodriguez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme de Espana SA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2303463A1 publication Critical patent/CA2303463A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une méthode de différenciation entre des bactéries provenant de taxa de maduromycètes et de streptomycètes utilisant une sonde d'acide nucléique spécifique complémentaire d'une région conservée des gènes d'ARNr 16S de maduromycètes. La sonde permet la détection rapide d'ADN codant pour l'ARNr 16S dans un échantillon différenciant les streptomycètes des maduromycètes de l'ordre des Actinomycetales. La méthode est précise et reproductible.
CA002303463A 1997-09-18 1998-09-16 Sondes d'hybridation destinees a differencier les streptomycetes des maduromycetes Abandoned CA2303463A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US5929597P 1997-09-18 1997-09-18
US60/059,295 1997-09-18
US6974897P 1997-12-16 1997-12-16
US60/069,748 1997-12-16
PCT/EP1998/006038 WO1999014361A1 (fr) 1997-09-18 1998-09-16 Sondes d'hybridation destinees a differencier les streptomycetes des maduromycetes

Publications (1)

Publication Number Publication Date
CA2303463A1 true CA2303463A1 (fr) 1999-03-25

Family

ID=26738593

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002303463A Abandoned CA2303463A1 (fr) 1997-09-18 1998-09-16 Sondes d'hybridation destinees a differencier les streptomycetes des maduromycetes

Country Status (4)

Country Link
EP (1) EP1012346A1 (fr)
JP (1) JP2001516590A (fr)
CA (1) CA2303463A1 (fr)
WO (1) WO1999014361A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6821770B1 (en) 1999-05-03 2004-11-23 Gen-Probe Incorporated Polynucleotide matrix-based method of identifying microorganisms
DE60028758T2 (de) 1999-05-03 2007-05-24 Gen-Probe Inc., San Diego Polynukleotidsonden zur detektion und quantifizierung von actinomycetes
WO2001023608A2 (fr) * 1999-09-27 2001-04-05 Merck Sharp & Dohme De Espana, S.A.E. Sondes d'hybridation permettant de detecter de maniere specifique des souches du genre microbispora, microtetraspora, nonomuria et planobispora

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms

Also Published As

Publication number Publication date
JP2001516590A (ja) 2001-10-02
WO1999014361A1 (fr) 1999-03-25
EP1012346A1 (fr) 2000-06-28

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Legal Events

Date Code Title Description
FZDE Discontinued