CA2303790A1 - Caracterisation d'acide nucleique par spectrometrie de masse - Google Patents
Caracterisation d'acide nucleique par spectrometrie de masse Download PDFInfo
- Publication number
- CA2303790A1 CA2303790A1 CA002303790A CA2303790A CA2303790A1 CA 2303790 A1 CA2303790 A1 CA 2303790A1 CA 002303790 A CA002303790 A CA 002303790A CA 2303790 A CA2303790 A CA 2303790A CA 2303790 A1 CA2303790 A1 CA 2303790A1
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- CA
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- Prior art keywords
- mass
- nucleic acid
- fragments
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- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 65
- 238000004949 mass spectrometry Methods 0.000 title claims abstract description 21
- 108020004707 nucleic acids Proteins 0.000 title claims description 52
- 102000039446 nucleic acids Human genes 0.000 title claims description 52
- 239000012634 fragment Substances 0.000 claims abstract description 68
- 238000000034 method Methods 0.000 claims abstract description 60
- 238000006243 chemical reaction Methods 0.000 claims description 55
- 238000013467 fragmentation Methods 0.000 claims description 53
- 238000006062 fragmentation reaction Methods 0.000 claims description 53
- 239000002773 nucleotide Substances 0.000 claims description 35
- 125000003729 nucleotide group Chemical group 0.000 claims description 35
- 238000007480 sanger sequencing Methods 0.000 claims description 15
- 238000002372 labelling Methods 0.000 claims description 5
- 230000015556 catabolic process Effects 0.000 claims description 2
- 238000001360 collision-induced dissociation Methods 0.000 claims description 2
- 238000006731 degradation reaction Methods 0.000 claims description 2
- 238000011176 pooling Methods 0.000 claims description 2
- 108010062513 snake venom phosphodiesterase I Proteins 0.000 claims description 2
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 53
- 150000002500 ions Chemical class 0.000 description 51
- 238000012163 sequencing technique Methods 0.000 description 42
- 238000004458 analytical method Methods 0.000 description 39
- 238000003776 cleavage reaction Methods 0.000 description 18
- 230000007017 scission Effects 0.000 description 17
- 241000894007 species Species 0.000 description 13
- 238000005040 ion trap Methods 0.000 description 12
- 238000000926 separation method Methods 0.000 description 12
- 238000004885 tandem mass spectrometry Methods 0.000 description 12
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 10
- 238000009396 hybridization Methods 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 238000004513 sizing Methods 0.000 description 9
- 108091034117 Oligonucleotide Proteins 0.000 description 8
- 238000013459 approach Methods 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 238000001819 mass spectrum Methods 0.000 description 7
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- 238000012986 modification Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 5
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 5
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- BRARRAHGNDUELT-UHFFFAOYSA-N 3-hydroxypicolinic acid Chemical compound OC(=O)C1=NC=CC=C1O BRARRAHGNDUELT-UHFFFAOYSA-N 0.000 description 4
- 108091028664 Ribonucleotide Proteins 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 4
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- 238000010367 cloning Methods 0.000 description 4
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- 238000003199 nucleic acid amplification method Methods 0.000 description 4
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- 239000002336 ribonucleotide Substances 0.000 description 4
- 125000002652 ribonucleotide group Chemical group 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
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- WXTMDXOMEHJXQO-UHFFFAOYSA-N 2,5-dihydroxybenzoic acid Chemical compound OC(=O)C1=CC(O)=CC=C1O WXTMDXOMEHJXQO-UHFFFAOYSA-N 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108091029865 Exogenous DNA Proteins 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 150000001793 charged compounds Chemical class 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000003795 desorption Methods 0.000 description 2
- 230000005672 electromagnetic field Effects 0.000 description 2
- 238000000132 electrospray ionisation Methods 0.000 description 2
- 230000007515 enzymatic degradation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- -1 nucleotide triphosphates Chemical class 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 108090001008 Avidin Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 238000004252 FT/ICR mass spectrometry Methods 0.000 description 1
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical group C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 239000002800 charge carrier Substances 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001851 cinnamic acid derivatives Chemical class 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 125000006575 electron-withdrawing group Chemical group 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000003682 fluorination reaction Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 239000013628 high molecular weight specie Substances 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000001616 ion spectroscopy Methods 0.000 description 1
- 238000000752 ionisation method Methods 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 238000012177 large-scale sequencing Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 238000012108 two-stage analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6872—Methods for sequencing involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un procédé permettant d'analyser une population de fragments d'acide nucléiques tous marqués par un marqueur de masse. Ce procédé consiste: i.) à ioniser la population; ii.) à trier en sous-populations, en fonction de la masse, la population ionisée dans un spectromètre de masse, chaque sous-population contenant au moins un fragment marqué; iii.) à cliver chaque population de façon à détacher le marqueur de masse associé à chaque fragment marqué; iv.) à évaluer par spectroscopie de masse la masse de chaque marqueur de masse détaché; et v.) à affecter chaque marqueur de masse au fragment qui lui est associé.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9719638.0A GB9719638D0 (en) | 1997-09-15 | 1997-09-15 | High throughput analysis of sanger dna ladders by tandem mass spectrometry |
| GB9719638.0 | 1997-09-15 | ||
| GB9725630.9 | 1997-12-03 | ||
| GBGB9725630.9A GB9725630D0 (en) | 1997-12-03 | 1997-12-03 | Charecterising nucleic acid |
| PCT/GB1998/002789 WO1999014362A1 (fr) | 1997-09-15 | 1998-09-15 | Caracterisation d'acide nucleique par spectrometrie de masse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2303790A1 true CA2303790A1 (fr) | 1999-03-25 |
Family
ID=26312254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002303790A Abandoned CA2303790A1 (fr) | 1997-09-15 | 1998-09-15 | Caracterisation d'acide nucleique par spectrometrie de masse |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1015632A1 (fr) |
| JP (1) | JP2001516591A (fr) |
| AU (1) | AU746443B2 (fr) |
| CA (1) | CA2303790A1 (fr) |
| NZ (1) | NZ503289A (fr) |
| WO (1) | WO1999014362A1 (fr) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5795714A (en) | 1992-11-06 | 1998-08-18 | Trustees Of Boston University | Method for replicating an array of nucleic acid probes |
| EP1164203B1 (fr) | 1996-11-06 | 2007-10-10 | Sequenom, Inc. | Diagnostics de l'ADN fondés sur la spectrométrie de masse |
| US6312904B1 (en) * | 1997-07-11 | 2001-11-06 | Xzillion Gmbh & Co. Kg | Characterizing nucleic acid |
| DE19824280B4 (de) | 1998-05-29 | 2004-08-19 | Bruker Daltonik Gmbh | Mutationsanalyse mittels Massenspektrometrie |
| GB0006141D0 (en) | 2000-03-14 | 2000-05-03 | Brax Group Ltd | Mass labels |
| DE60137722D1 (de) * | 2000-06-13 | 2009-04-02 | Univ Boston | Verwendung von mass-matched nukleotide in der analyse von oligonukleotidmischungen sowie in der hoch-multiplexen nukleinsäuresequenzierung |
| PT1425586E (pt) | 2001-09-14 | 2007-12-31 | Electrophoretics Ltd | Marcadores de massa |
| US20040219685A1 (en) | 2003-01-30 | 2004-11-04 | Applera Corporation | Methods and mixtures pertaining to analyte determination using electrophilic labeling reagents |
| US20050148087A1 (en) | 2004-01-05 | 2005-07-07 | Applera Corporation | Isobarically labeled analytes and fragment ions derived therefrom |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69433811T2 (de) * | 1993-01-07 | 2005-06-23 | Sequenom, Inc., San Diego | Dns - sequenzierung durch massenspektronomie |
| CA2158642A1 (fr) * | 1993-03-19 | 1994-09-29 | Hubert Koster | Sequencage de l'adn par spectrometrie de masse, via la degradation de l'exonuclease |
| GB9504598D0 (en) * | 1995-03-03 | 1995-04-26 | Imp Cancer Res Tech | Method of nucleic acid analysis |
| DE69701671T3 (de) * | 1996-01-23 | 2006-08-17 | Qiagen Genomics, Inc., Bothell | Verfahren und zusammenstellungen zur sequenzbestimmung von nukleinsäuremolekülen |
| AU2069597A (en) * | 1996-03-04 | 1997-09-22 | Genetrace Systems, Inc. | Methods of screening nucleic acids using mass spectrometry |
| WO1997037041A2 (fr) * | 1996-03-18 | 1997-10-09 | Sequenom, Inc. | Sequençage d'adn par spectrometrie de masse |
| US5803916A (en) * | 1996-03-19 | 1998-09-08 | Vital-Tech Ltd. | Body and joints massage device |
| GB9620769D0 (en) * | 1996-10-04 | 1996-11-20 | Brax Genomics Ltd | Nucleic acid sequencing |
| EP0963443B1 (fr) * | 1996-12-10 | 2006-03-08 | Sequenom, Inc. | Molecules d'etiquetage massique, non volatiles et liberables |
| JP3884087B2 (ja) * | 1997-01-15 | 2007-02-21 | イクスツィリオン ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 質量標識結合ハイブリダイゼーションプローブ |
| AU5974498A (en) * | 1997-02-18 | 1998-09-09 | Kinetic Limited | Control arrangement for vehicle suspension system |
-
1998
- 1998-09-15 CA CA002303790A patent/CA2303790A1/fr not_active Abandoned
- 1998-09-15 WO PCT/GB1998/002789 patent/WO1999014362A1/fr not_active Ceased
- 1998-09-15 JP JP2000511900A patent/JP2001516591A/ja active Pending
- 1998-09-15 EP EP98942924A patent/EP1015632A1/fr not_active Withdrawn
- 1998-09-15 AU AU90887/98A patent/AU746443B2/en not_active Ceased
- 1998-09-15 NZ NZ503289A patent/NZ503289A/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1015632A1 (fr) | 2000-07-05 |
| AU9088798A (en) | 1999-04-05 |
| JP2001516591A (ja) | 2001-10-02 |
| WO1999014362A1 (fr) | 1999-03-25 |
| NZ503289A (en) | 2002-11-26 |
| AU746443B2 (en) | 2002-05-02 |
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