CA2304929C - Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose - Google Patents

Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose Download PDF

Info

Publication number: CA2304929C
Authority: CA; Canada
Prior art keywords: operon; bacterium; foreign genes; xylose; tal
Prior art date: 2000-05-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002304929A

Other languages

English (en)

Other versions

CA2304929A1 (fr

Inventor

Min Zhang

Yat-Chen Chou

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Alliance for Sustainable Energy LLC

Original Assignee

Alliance for Sustainable Energy LLC

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-05-02

Filing date

2000-05-02

Publication date

2009-12-22

2000-05-02 Priority to CA002304929A priority Critical patent/CA2304929C/fr

2000-05-02 Application filed by Alliance for Sustainable Energy LLC filed Critical Alliance for Sustainable Energy LLC

2001-04-06 Priority to AU4992601A priority patent/AU4992601A/xx

2001-04-06 Priority to PCT/US2001/011334 priority patent/WO2001083786A2/fr

2001-04-06 Priority to EP01923213A priority patent/EP1278876A2/fr

2001-04-06 Priority to AU2001249926A priority patent/AU2001249926B2/en

2001-04-06 Priority to BRPI0110987-1A priority patent/BR0110987B1/pt

2001-04-06 Priority to JP2001580393A priority patent/JP4510355B2/ja

2001-11-02 Publication of CA2304929A1 publication Critical patent/CA2304929A1/fr

2009-12-22 Application granted granted Critical

2009-12-22 Publication of CA2304929C publication Critical patent/CA2304929C/fr

2020-05-02 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

241000588902 Zymomonas mobilis Species 0.000 title claims abstract description 43
SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 title claims description 137
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 title claims description 122
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 title claims description 121
PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 title claims description 37
108090000623 proteins and genes Proteins 0.000 claims abstract description 83
239000012634 fragment Substances 0.000 claims abstract description 38
238000003780 insertion Methods 0.000 claims abstract description 35
230000037431 insertion Effects 0.000 claims abstract description 35
241000894006 Bacteria Species 0.000 claims abstract description 28
108010020764 Transposases Proteins 0.000 claims abstract description 19
230000001580 bacterial effect Effects 0.000 claims abstract description 13
230000014509 gene expression Effects 0.000 claims abstract description 13
102000004190 Enzymes Human genes 0.000 claims abstract description 12
108090000790 Enzymes Proteins 0.000 claims abstract description 12
101150106193 tal gene Proteins 0.000 claims description 26
101100536311 Drosophila melanogaster Taldo gene Proteins 0.000 claims description 25
101100480526 Drosophila melanogaster tal-1A gene Proteins 0.000 claims description 25
101100205917 Drosophila melanogaster tal-2A gene Proteins 0.000 claims description 25
101100312937 Drosophila melanogaster tal-3A gene Proteins 0.000 claims description 25
101100312939 Drosophila melanogaster tal-AA gene Proteins 0.000 claims description 25
101100098715 Mus musculus Taldo1 gene Proteins 0.000 claims description 25
101100205913 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) tal1 gene Proteins 0.000 claims description 25
241000588901 Zymomonas Species 0.000 claims description 24
238000002744 homologous recombination Methods 0.000 claims description 14
230000006801 homologous recombination Effects 0.000 claims description 14
101150104734 ldh gene Proteins 0.000 claims description 14
239000013598 vector Substances 0.000 claims description 14
101100454398 Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) ldh1 gene Proteins 0.000 claims description 13
101150110790 xylB gene Proteins 0.000 claims description 13
101150024271 TKT gene Proteins 0.000 claims description 12
101100157012 Thermoanaerobacterium saccharolyticum (strain DSM 8691 / JW/SL-YS485) xynB gene Proteins 0.000 claims description 12
101100537319 Escherichia coli (strain K12) tktA gene Proteins 0.000 claims description 7
101100170068 Mus musculus Ddr2 gene Proteins 0.000 claims description 7
101150097746 araB gene Proteins 0.000 claims description 6
OIRDTQYFTABQOQ-UHTZMRCNSA-N Vidarabine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@@H]1O OIRDTQYFTABQOQ-UHTZMRCNSA-N 0.000 claims description 5
OIRDTQYFTABQOQ-UHFFFAOYSA-N ara-adenosine Natural products Nc1ncnc2n(cnc12)C1OC(CO)C(O)C1O OIRDTQYFTABQOQ-UHFFFAOYSA-N 0.000 claims description 5
101150035354 araA gene Proteins 0.000 claims description 5
101150017736 araD gene Proteins 0.000 claims description 5
101150011516 xlnD gene Proteins 0.000 claims description 5
101150052264 xylA gene Proteins 0.000 claims description 5
239000011541 reaction mixture Substances 0.000 claims description 2
101100429087 Bacillus subtilis (strain 168) xlyA gene Proteins 0.000 claims 1
239000013612 plasmid Substances 0.000 abstract description 92
238000000855 fermentation Methods 0.000 abstract description 16
230000004151 fermentation Effects 0.000 abstract description 15
235000000346 sugar Nutrition 0.000 abstract description 13
238000006243 chemical reaction Methods 0.000 abstract description 7
239000001913 cellulose Substances 0.000 abstract description 6
229920002678 cellulose Polymers 0.000 abstract description 6
239000000446 fuel Substances 0.000 abstract description 5
-1 pentose sugars Chemical class 0.000 abstract description 5
239000000126 substance Substances 0.000 abstract description 5
239000013605 shuttle vector Substances 0.000 abstract description 4
230000009466 transformation Effects 0.000 abstract description 4
238000005516 engineering process Methods 0.000 abstract description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 46
108020004414 DNA Proteins 0.000 description 39
239000000523 sample Substances 0.000 description 27
238000000034 method Methods 0.000 description 21
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 20
239000008103 glucose Substances 0.000 description 20
241000588724 Escherichia coli Species 0.000 description 19
SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 17
108020004707 nucleic acids Proteins 0.000 description 16
150000007523 nucleic acids Chemical class 0.000 description 16
102000039446 nucleic acids Human genes 0.000 description 16
101000851432 Escherichia coli Transposase for transposon Tn5 Proteins 0.000 description 15
102100029089 Xylulose kinase Human genes 0.000 description 15
230000010354 integration Effects 0.000 description 15
108091022915 xylulokinase Proteins 0.000 description 15
102100028601 Transaldolase Human genes 0.000 description 14
108020004530 Transaldolase Proteins 0.000 description 14
210000004027 cell Anatomy 0.000 description 14
238000003752 polymerase chain reaction Methods 0.000 description 14
230000017105 transposition Effects 0.000 description 14
102000014701 Transketolase Human genes 0.000 description 13
108010043652 Transketolase Proteins 0.000 description 13
108700040099 Xylose isomerases Proteins 0.000 description 13
238000004458 analytical method Methods 0.000 description 13
238000010367 cloning Methods 0.000 description 12
230000002255 enzymatic effect Effects 0.000 description 12
230000013011 mating Effects 0.000 description 12
239000002609 medium Substances 0.000 description 10
230000008569 process Effects 0.000 description 10
238000002372 labelling Methods 0.000 description 9
239000000047 product Substances 0.000 description 9
238000010276 construction Methods 0.000 description 8
239000000758 substrate Substances 0.000 description 8
238000012546 transfer Methods 0.000 description 8
101150038013 PIR gene Proteins 0.000 description 7
238000002105 Southern blotting Methods 0.000 description 7
230000003321 amplification Effects 0.000 description 7
230000000694 effects Effects 0.000 description 7
229940088598 enzyme Drugs 0.000 description 7
238000003199 nucleic acid amplification method Methods 0.000 description 7
108010018080 L-arabinose isomerase Proteins 0.000 description 6
102000008579 Transposases Human genes 0.000 description 6
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
238000009396 hybridization Methods 0.000 description 6
108020002667 ribulokinase Proteins 0.000 description 6
239000004098 Tetracycline Substances 0.000 description 5
230000003115 biocidal effect Effects 0.000 description 5
230000021615 conjugation Effects 0.000 description 5
230000029087 digestion Effects 0.000 description 5
230000012010 growth Effects 0.000 description 5
239000003550 marker Substances 0.000 description 5
MHWLWQUZZRMNGJ-UHFFFAOYSA-N nalidixic acid Chemical compound C1=C(C)N=C2N(CC)C=C(C(O)=O)C(=O)C2=C1 MHWLWQUZZRMNGJ-UHFFFAOYSA-N 0.000 description 5
229960000210 nalidixic acid Drugs 0.000 description 5
229960002180 tetracycline Drugs 0.000 description 5
229930101283 tetracycline Natural products 0.000 description 5
235000019364 tetracycline Nutrition 0.000 description 5
150000003522 tetracyclines Chemical class 0.000 description 5
101150014795 tktA gene Proteins 0.000 description 5
229920001817 Agar Polymers 0.000 description 4
239000008272 agar Substances 0.000 description 4
238000003556 assay Methods 0.000 description 4
238000004520 electroporation Methods 0.000 description 4
239000000203 mixture Substances 0.000 description 4
238000002703 mutagenesis Methods 0.000 description 4
231100000350 mutagenesis Toxicity 0.000 description 4
239000013600 plasmid vector Substances 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
239000006152 selective media Substances 0.000 description 4
150000008163 sugars Chemical class 0.000 description 4
239000002028 Biomass Substances 0.000 description 3
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
108090000416 L-ribulose-5-phosphate 4-epimerases Proteins 0.000 description 3
108020004511 Recombinant DNA Proteins 0.000 description 3
229910052799 carbon Inorganic materials 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
239000000463 material Substances 0.000 description 3
230000001404 mediated effect Effects 0.000 description 3
HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 3
244000005700 microbiome Species 0.000 description 3
230000003287 optical effect Effects 0.000 description 3
238000012360 testing method Methods 0.000 description 3
101150064636 tnp gene Proteins 0.000 description 3
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
UJOBWOGCFQCDNV-UHFFFAOYSA-N 9H-carbazole Chemical compound C1=CC=C2C3=CC=CC=C3NC2=C1 UJOBWOGCFQCDNV-UHFFFAOYSA-N 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
206010010144 Completed suicide Diseases 0.000 description 2
229920002488 Hemicellulose Polymers 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
PYMYPHUHKUWMLA-VPENINKCSA-N aldehydo-D-xylose Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-VPENINKCSA-N 0.000 description 2
101150029595 ara gene Proteins 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
230000002759 chromosomal effect Effects 0.000 description 2
210000000349 chromosome Anatomy 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
238000007824 enzymatic assay Methods 0.000 description 2
239000000284 extract Substances 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
229940012229 genone Drugs 0.000 description 2
238000004128 high performance liquid chromatography Methods 0.000 description 2
239000000413 hydrolysate Substances 0.000 description 2
239000002054 inoculum Substances 0.000 description 2
230000001320 lysogenic effect Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
230000004108 pentose phosphate pathway Effects 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
108091008146 restriction endonucleases Proteins 0.000 description 2
230000010473 stable expression Effects 0.000 description 2
230000024540 transposon integration Effects 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
229920000936 Agarose Polymers 0.000 description 1
101100148606 Caenorhabditis elegans pst-1 gene Proteins 0.000 description 1
244000025254 Cannabis sativa Species 0.000 description 1
238000007399 DNA isolation Methods 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
101100285518 Drosophila melanogaster how gene Proteins 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241000644323 Escherichia coli C Species 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
101710150114 Protein rep Proteins 0.000 description 1
101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
108700005075 Regulator Genes Proteins 0.000 description 1
108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
101710152114 Replication protein Proteins 0.000 description 1
241000428533 Rhis Species 0.000 description 1
241000702208 Shigella phage SfX Species 0.000 description 1
101100518964 Sphingobium sp. (strain YBL2) parT gene Proteins 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
230000003698 anagen phase Effects 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000011942 biocatalyst Substances 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
239000006227 byproduct Substances 0.000 description 1
230000025938 carbohydrate utilization Effects 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000012217 deletion Methods 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000000835 fiber Substances 0.000 description 1
230000004927 fusion Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
229930027917 kanamycin Natural products 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
229930182823 kanamycin A Natural products 0.000 description 1
238000011031 large-scale manufacturing process Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
239000012528 membrane Substances 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000009343 monoculture Methods 0.000 description 1
230000001937 non-anti-biotic effect Effects 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
230000027086 plasmid maintenance Effects 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
230000008092 positive effect Effects 0.000 description 1
230000006798 recombination Effects 0.000 description 1
238000005215 recombination Methods 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
239000002689 soil Substances 0.000 description 1
238000000527 sonication Methods 0.000 description 1
101150040618 talB gene Proteins 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000000811 xylitol Substances 0.000 description 1
HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
229960002675 xylitol Drugs 0.000 description 1
235000010447 xylitol Nutrition 0.000 description 1
150000003741 xylose derivatives Chemical class 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Mycology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA002304929A 2000-05-01 2000-05-02 Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose Expired - Lifetime CA2304929C (fr)

Priority Applications (7)

Application Number	Priority Date	Filing Date	Title
CA002304929A CA2304929C (fr)	2000-05-02	2000-05-02	Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose
PCT/US2001/011334 WO2001083786A2 (fr)	2000-05-01	2001-04-06	Souches de $i(zymomonas mobilis) stables induisant la fermentation du xylose et de l'arabinose
EP01923213A EP1278876A2 (fr)	2000-05-01	2001-04-06	Souches de $i(zymomonas mobilis) stables induisant la fermentation du xylose et de l'arabinose
AU2001249926A AU2001249926B2 (en)	2000-05-01	2001-04-06	Stable zymomonas mobilis xylose and arabinose fermenting strains
AU4992601A AU4992601A (en)	2000-05-01	2001-04-06	Stable zymomonas mobilis xylose and arabinose fermenting strains
BRPI0110987-1A BR0110987B1 (pt)	2000-05-01	2001-04-06	Transposon para inserção estável de genes alheios dentro de um genoma bacteriano, vetor plasmídio, bactéria, e, mistura de reação compreendendo um meio fermentável
JP2001580393A JP4510355B2 (ja)	2000-05-01	2001-04-06	安定なチモモナスモビリスのキシロースおよびアラビノース醗酵株

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA002304929A CA2304929C (fr)	2000-05-02	2000-05-02	Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose

Publications (2)

Publication Number	Publication Date
CA2304929A1 CA2304929A1 (fr)	2001-11-02
CA2304929C true CA2304929C (fr)	2009-12-22

Family

ID=4165850

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002304929A Expired - Lifetime CA2304929C (fr)	2000-05-01	2000-05-02	Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose

Country Status (1)

Country	Link
CA (1)	CA2304929C (fr)

2000
- 2000-05-02 CA CA002304929A patent/CA2304929C/fr not_active Expired - Lifetime

Also Published As

Publication number	Publication date
CA2304929A1 (fr)	2001-11-02

Legal Events

Date	Code	Title	Description
2000-10-03	EEER	Examination request
2020-07-05	MKEX	Expiry	Effective date: 20200502

Publication	Publication Date	Title
EP1549736B1 (fr)	2009-04-15	Souches de fermentation de sucre pentose de zymomonas et utilisations associees
US7354755B2 (en)	2008-04-08	Stable zymomonas mobilis xylose and arabinose fermenting strains
AU2001249926B2 (en)	2006-07-20	Stable zymomonas mobilis xylose and arabinose fermenting strains
AU2001249926A1 (en)	2002-01-31	Stable zymomonas mobilis xylose and arabinose fermenting strains
JP2009065982A (ja)	2009-04-02	エタノール産生
AU2001251397B2 (en)	2006-06-22	Method of site-specific insertion in zymomonas mobilis
US8097460B2 (en)	2012-01-17	Ethanol production in bacillus
AU2001251397A1 (en)	2002-01-31	Method of site-specific insertion in zymomonas mobilis
CA2304929C (fr)	2009-12-22	Souches stables de zymomonas mobilis pour la fermentation du xylose et de l'arabinose
Zhang et al.	2008	Stable Zymomonas mobilis xylose and arabinose fermenting strains
AU2003299464B2 (en)	2007-09-06	Zymomonas pentose-sugar fermentating strains and uses thereof
US8735160B2 (en)	2014-05-27	Methods for targetted mutagenesis in gram-positive bacteria
AU2007231884B2 (en)	2012-06-07	Ethanol production
HK1143834A (en)	2011-01-14	Ethanol production