CA2305341A1 - Stabilization of envelope glycoprotein trimers by disulfide bonds introduced into a gp41 glycoprotein ectodomain - Google Patents

Stabilization of envelope glycoprotein trimers by disulfide bonds introduced into a gp41 glycoprotein ectodomain Download PDF

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Publication number
CA2305341A1
CA2305341A1 CA002305341A CA2305341A CA2305341A1 CA 2305341 A1 CA2305341 A1 CA 2305341A1 CA 002305341 A CA002305341 A CA 002305341A CA 2305341 A CA2305341 A CA 2305341A CA 2305341 A1 CA2305341 A1 CA 2305341A1
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CA
Canada
Prior art keywords
trimers
glycoprotein
disulfide bonds
ectodomain
stabilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002305341A
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French (fr)
Other versions
CA2305341C (en
Inventor
Joseph G. Sodroski
Michael Farzan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dana Farber Cancer Institute Inc
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Individual
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Publication date
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Publication of CA2305341A1 publication Critical patent/CA2305341A1/en
Application granted granted Critical
Publication of CA2305341C publication Critical patent/CA2305341C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • AIDS & HIV (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present application is directed to stabilized envelope glycoprotein trimers. The trimers are stabilized by introducing disulfide bonds at certain sites in the gp41 ectodomain. DNA molecules encoding such trimers can be used to generate an immunogenic reaction.
CA002305341A 1997-10-01 1998-10-01 Stabilization of envelope glycoprotein trimers by disulfide bonds introduced into a gp41 glycoprotein ectodomain Expired - Fee Related CA2305341C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6081397P 1997-10-01 1997-10-01
US60/060,813 1997-10-01
US6080897P 1997-10-03 1997-10-03
US60/060,808 1997-10-03
PCT/US1998/020693 WO1999016883A2 (en) 1997-10-01 1998-10-01 STABILIZATION OF ENVELOPE GLYCOPROTEIN TRIMERS BY DISULFIDE BONDS INTRODUCED INTO A gp41 GLYCOPROTEIN ECTODOMAIN

Publications (2)

Publication Number Publication Date
CA2305341A1 true CA2305341A1 (en) 1999-04-08
CA2305341C CA2305341C (en) 2009-01-06

Family

ID=26740384

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002305341A Expired - Fee Related CA2305341C (en) 1997-10-01 1998-10-01 Stabilization of envelope glycoprotein trimers by disulfide bonds introduced into a gp41 glycoprotein ectodomain

Country Status (4)

Country Link
EP (1) EP1019511A2 (en)
AU (1) AU9678598A (en)
CA (1) CA2305341C (en)
WO (1) WO1999016883A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2781676B1 (en) * 1998-07-31 2004-04-02 Pasteur Merieux Serums Vacc QUARTER OF HIV ENV GENE EXPRESSION PRODUCT
WO2000039302A2 (en) 1998-12-31 2000-07-06 Chiron Corporation Improved expression of hiv polypeptides and production of virus-like particles
WO2001019958A2 (en) 1999-09-17 2001-03-22 Dana-Farber Cancer Institute, Inc. Stabilized soluble glycoprotein trimers
US7179468B1 (en) 2000-06-08 2007-02-20 Cornell Research Foundation, Inc. Antigen for developing neutralizing antibodies to human immunodeficiency virus
WO2002056831A2 (en) 2000-12-27 2002-07-25 Dana-Farber Cancer Institute, Inc. Immunogenic proteoliposomes, and uses thereof
AU2002320314A1 (en) 2001-07-05 2003-01-21 Chiron, Corporation Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof
US20030170614A1 (en) 2001-08-31 2003-09-11 Megede Jan Zur Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof
EP1755667B1 (en) * 2004-06-01 2013-07-17 Merck Sharp & Dohme Corp. Stable peptide mimetic of hiv gp41 fusion intermediate
ES2324755B1 (en) * 2007-04-04 2010-05-31 Consejo Superior De Investigaciones Cientificas (Csic) PROTEIN GP120 POLYPEPTIDE ABLE TO INHIBIT THE VITAL CYCLE OF AIDS VIRUS.
US9217011B2 (en) 2007-09-07 2015-12-22 Complix Nv Non-natural proteinaceous scaffold made of three non-covalently associated peptides
EP2161278B1 (en) * 2008-09-08 2012-05-23 Complix N.V. Single-chain coiled coil scaffold
EP3303367B1 (en) * 2015-06-08 2025-06-18 New York University Stabilized minimal coiled-coil mimetics
US11891422B2 (en) 2018-11-16 2024-02-06 New York University NEMO coiled coil mimics and methods of using same
CN110184298B (en) * 2019-05-15 2023-05-16 武汉璟泓科技股份有限公司 HIV mutant type surface glycoprotein, nano antigen and preparation method thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704694A (en) 1985-12-16 1987-11-03 Automation Intelligence, Inc. Learned part system
US4976778A (en) 1988-03-08 1990-12-11 Scm Metal Products, Inc. Infiltrated powder metal part and method for making same
WO1993004701A1 (en) 1991-09-05 1993-03-18 University Of Connecticut Targeted delivery of poly- or oligonucleotides to cells
WO1995022618A1 (en) 1994-02-22 1995-08-24 Dana-Farber Cancer Institute Nucleic acid delivery system, method of synthesis and uses thereof

Also Published As

Publication number Publication date
WO1999016883A2 (en) 1999-04-08
CA2305341C (en) 2009-01-06
AU9678598A (en) 1999-04-23
EP1019511A2 (en) 2000-07-19
WO1999016883A3 (en) 1999-06-17

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