CA2307624A1 - Cellules souches hematopoietiques - Google Patents

Cellules souches hematopoietiques Download PDF

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Publication number
CA2307624A1
CA2307624A1 CA 2307624 CA2307624A CA2307624A1 CA 2307624 A1 CA2307624 A1 CA 2307624A1 CA 2307624 CA2307624 CA 2307624 CA 2307624 A CA2307624 A CA 2307624A CA 2307624 A1 CA2307624 A1 CA 2307624A1
Authority
CA
Canada
Prior art keywords
cells
lin
stem cells
human
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2307624
Other languages
English (en)
Inventor
John E. Dick
Dominique Bonnet
Mickie Bhatia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HSC Research and Development LP
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 2307624 priority Critical patent/CA2307624A1/fr
Publication of CA2307624A1 publication Critical patent/CA2307624A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des cellules souches hématopoïétiques humaines appelées CD34-Lin- et CD34-CD38-Lin- et des procédés d'isolement et d'utilisation de ces cellules dans des compositions et dans des méthodes de reconstitution d'une population cellulaire déficiente ou absente. La présente invention concerne également une population de cellules souches hématopoïétiques humaines pouvant être isolées et modifiées génétiquement et destinées à être introduites chez un patient humain, afin de corriger divers dysfonctionnements génétiques, mises en culture et encore différenciées in vitro pour obtenir une nouvelle population de cellules.
CA 2307624 1997-10-31 1998-10-30 Cellules souches hematopoietiques Abandoned CA2307624A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2307624 CA2307624A1 (fr) 1997-10-31 1998-10-30 Cellules souches hematopoietiques

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA2,219,869 1997-10-31
CA002219869A CA2219869A1 (fr) 1997-10-31 1997-10-31 Cellule de souche humaine cd-34 hematopoietique
PCT/CA1998/001012 WO1999023205A1 (fr) 1997-10-31 1998-10-30 Cellules souches hematopoietiques
CA 2307624 CA2307624A1 (fr) 1997-10-31 1998-10-30 Cellules souches hematopoietiques

Publications (1)

Publication Number Publication Date
CA2307624A1 true CA2307624A1 (fr) 1999-05-14

Family

ID=4161713

Family Applications (2)

Application Number Title Priority Date Filing Date
CA002219869A Abandoned CA2219869A1 (fr) 1997-10-31 1997-10-31 Cellule de souche humaine cd-34 hematopoietique
CA 2307624 Abandoned CA2307624A1 (fr) 1997-10-31 1998-10-30 Cellules souches hematopoietiques

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA002219869A Abandoned CA2219869A1 (fr) 1997-10-31 1997-10-31 Cellule de souche humaine cd-34 hematopoietique

Country Status (3)

Country Link
AU (1) AU9732098A (fr)
CA (2) CA2219869A1 (fr)
WO (1) WO1999023205A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7927587B2 (en) 1999-08-05 2011-04-19 Regents Of The University Of Minnesota MAPC administration for the treatment of lysosomal storage disorders
US6632620B1 (en) 2000-06-22 2003-10-14 Andrew N. Makarovskiy Compositions for identification and isolation of stem cells
WO2002039109A2 (fr) * 2000-11-07 2002-05-16 British Columbia Cancer Agency Procede de detection de cellules hematopoietiques humaines capables de reconstitution a court terme
US7799324B2 (en) 2001-12-07 2010-09-21 Geron Corporation Using undifferentiated embryonic stem cells to control the immune system
CN1630716A (zh) 2001-12-07 2005-06-22 杰龙公司 人胚胎干细胞衍生的造血细胞
EP1694345A1 (fr) 2003-12-04 2006-08-30 Regents Of The University Of Minnesota Compositions et methodes de traitement des maladies lysosomales
WO2008063675A2 (fr) 2006-11-24 2008-05-29 Regents Of The University Of Minnesota Cellules progénitrices endothermiques
EP2746769A1 (fr) * 2012-12-21 2014-06-25 Stembios Technologies, Inc. Procédé pour évaluer l'effet d'action sur un sujet basé sur des dynamiques cellulaires de tiges
ES2747951T3 (es) 2013-10-24 2020-03-12 Ospedale San Raffaele Srl Método
AU2019282761B2 (en) 2018-06-07 2024-01-04 The Brigham And Women's Hospital, Inc. Methods for generating hematopoietic stem cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2223492A1 (fr) * 1995-06-06 1996-12-12 The Whitehead Institute For Biomedical Research Isolation de cellules souches hematopo?etiques de mammaliennes

Also Published As

Publication number Publication date
AU9732098A (en) 1999-05-24
CA2219869A1 (fr) 1999-04-30
WO1999023205A1 (fr) 1999-05-14

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