CA2308111A1 - A method of improved mixing in roller bottles - Google Patents

A method of improved mixing in roller bottles Download PDF

Info

Publication number
CA2308111A1
CA2308111A1 CA002308111A CA2308111A CA2308111A1 CA 2308111 A1 CA2308111 A1 CA 2308111A1 CA 002308111 A CA002308111 A CA 002308111A CA 2308111 A CA2308111 A CA 2308111A CA 2308111 A1 CA2308111 A1 CA 2308111A1
Authority
CA
Canada
Prior art keywords
roller bottle
mixing
enhancing
materials
recited
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002308111A
Other languages
French (fr)
Other versions
CA2308111C (en
Inventor
Joye L. Bramble
James A. Searles
Fernando J. Muzzio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Merck Sharp and Dohme LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9727128.2A external-priority patent/GB9727128D0/en
Application filed by Individual filed Critical Individual
Publication of CA2308111A1 publication Critical patent/CA2308111A1/en
Application granted granted Critical
Publication of CA2308111C publication Critical patent/CA2308111C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C12M27/12—Roller bottles; Roller tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting

Landscapes

  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method for enhancing the mixing of materials in roller bottle by the introduction of controlled axial and cross-sectional flow perturbations is disclosed. The effectiveness of the method is demonstrated by achieving higher efficiency in cell culturing and virus propagation in roller bottles by introducing controlled flow perturbations during the process.

Claims (29)

1. A method for enhancing the mixing of materials comprising a cell culture, virus infected cells or a virus, and media needed to sustain the cell culture, virus infected cells, and the virus in a roller bottle which comprises the use of controlled axial flow perturbations, cross sectional flow perturbations, or both axial and cross-sectional flow perturbations in rotational roller bottle mixing.
2. The method for enhancing the mixing of the materials in a roller bottle as recited in Claim 1, wherein the controlled axial flow perturbations are introduced by a combination of a rocking motion in the vertical direction and constant rotation of the roller bottle along a horizontal axis.
3. The method for enhancing the mixing of the materials in a roller bottle as recited in Claim 2, wherein the rocking motion is generated by rocking the rollers that drive the roller bottle rotation.
4. The method for enhancing the mixing of the materials in a roller bottle as recited in Claim 3, wherein the rocking motion is defined by a rocking angle of about 0 degrees to about +10 degrees or -10 degrees, and a rock to roll frequency of about 0 to about 31.4.
5. The method for enhancing the mixing of the materials in a roller bottle as recited in Claim 2, wherein the rocking motion is generated by attaching a plurality of protuberances to the rollers that drive the roller bottle rotation.
6. The method for enhancing the mixing of the materials in a roller bottle as recited in Claim 2, wherein the rocking motion is generated by attaching a plurality of protuberances to the roller bottle.
7. The method for enhancing the mixing of the materials in a roller bottle as recited in Claim 1, wherein the controlled cross-sectional flow perturbations arc introduced by using a time-dependent speed of rotation.
8. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 7, wherein the speed of rotation is varied about 0.01 to about 10 times the frequency of bottle rotation.
9. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 7, wherein the speed of rotation is varied either continuously or discontinuously, with a time dependent frequency.
10. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 1, wherein the controlled cross-sectional flow perturbations are introduced by reversing the direction of bottle rotation.
11. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 10, wherein the direction of rotation is varied about 0.01 to about 3 times the frequency of roller bottle rotation.
12. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 10, wherein the direction of rotation is varied either continuously or discontinuously, with a time dependent frequency.
13. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 1, wherein the controlled axial and cross-sectional flow perturbations are introduced by combining a rocking motion with time-dependent speed of rotation.
14. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 13, wherein the rocking motion is generated by rocking the rollers that drive roller bottle rotation.
15. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 13, wherein the rocking motion is generated by attaching a protuberance to the rollers that drive the roller bottle rotation.
16. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 13, wherein the rocking motion is generated by attaching a protuberance to the roller bottle.
17. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 13, wherein the rocking motion is defined by a rocking angle of about 0 degrees to about + 10 degrees or - 10 degrees, and a rock to roll frequency of about 0 to about 31.4 and a time-dependent rotation speed varied a frequency of about 0.01 to about 10 times the roller bottle rotation speed.
18. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 17, wherein the rocking speed and the speed of rotation are varied, either continuously or discontinuously, with a time-dependent frequency.
19. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 1, wherein the controlled axial and cross-sectional flow perturbations are introduced by combining rocking motion and time-dependent direction of rotation.
20. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 19, wherein the rocking motion is generated by rocking the rollers that drive roller bottle rotation.
21. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 19, wherein the rocking motion is generated by attaching a protuberance to the rollers that drive the roller bottle rotation.
22. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 19, wherein the rocking motion is generated by attaching a protuberance to the roller bottle.
23. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 19, wherein the rocking motion is defined by a rocking angle of about 0 degrees to about + 10 degrees or about - 10 degrees, and a rock to roll frequency of about 0 to about 31.4, and a time-dependent direction which is varied about 0.01 to about 3 times the frequency of roller bottle rotation.
24. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 23, wherein the rocking speed and the direction of rotation are varied, either continuously or discontinuously, with a time-dependent frequency.
25. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 1, wherein the controlled axial and cross sectional flow perturbations are introduced by combining a rocking motion, a time dependent speed of rotation, and a time-dependent direction of rotation.
26. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 25, wherein the rocking motion is generated by rocking the rollers that drive roller bottle rotation.
27. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 25, wherein the rocking motion is generated by attaching a protuberance to the rollers that drive roller bottle rotation.
28. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 25, wherein the rocking motion is generated by attaching a protuberance to the roller bottle.
29. The method for enhancing the mixing of materials in a roller bottle as recited in Claim 25, wherein the rocking motion is defined by a rocking angle of about 0 degrees to about + 10 or about - 10 degrees and a rock to roll frequency of about 0 to about 31.4, and the time dependent direction of rotation is varied about 0.01 to about 3 times the frequency of roller bottle rotation and the speed of rotation is varied a frequency of about 0.01 to about 10 times the frequency of roller bottle rotation.
CA2308111A 1997-10-31 1998-10-27 A method of improved mixing in roller bottles Expired - Fee Related CA2308111C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US6385497P 1997-10-31 1997-10-31
US60/063,854 1997-10-31
US6737197P 1997-12-03 1997-12-03
US60/067,371 1997-12-03
GB9727128.2 1997-12-22
GBGB9727128.2A GB9727128D0 (en) 1997-12-22 1997-12-22 A method of improved mixing in roller bottles
PCT/US1998/022729 WO1999023204A1 (en) 1997-10-31 1998-10-27 A method of improved mixing in roller bottles

Publications (2)

Publication Number Publication Date
CA2308111A1 true CA2308111A1 (en) 1999-05-14
CA2308111C CA2308111C (en) 2010-12-21

Family

ID=27269148

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2308111A Expired - Fee Related CA2308111C (en) 1997-10-31 1998-10-27 A method of improved mixing in roller bottles

Country Status (5)

Country Link
EP (1) EP1027425A4 (en)
JP (1) JP2001521736A (en)
AU (1) AU737705B2 (en)
CA (1) CA2308111C (en)
WO (1) WO1999023204A1 (en)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3091435A (en) * 1961-01-30 1963-05-28 Curtiss Wright Corp Rotary-oscillatory device for mixing, tumbling, comminuting, and the like
DE1940865B2 (en) * 1968-08-10 1975-04-17 Monrick Holdings Ltd., Downsview, Ontario (Kanada) Device for processing photographic material with a processing liquid
DE1957069B2 (en) * 1969-11-13 1973-07-26 Merz, Werner, 7911 Oberelchingen DEVELOPMENT DEVICE FOR SHEET-SHAPED PHOTOGRAPHIC FILM MATERIALS
US3982259A (en) * 1974-12-13 1976-09-21 Edward Van Baerle Photographic material processing module
US3981488A (en) * 1975-01-02 1976-09-21 Monrick Holdings Limited Carrier for processing photographic material and apparatus for rotating the carrier
DE2934328C2 (en) * 1979-08-24 1982-04-29 Stephan Dipl.-Chem. Dr.rer.nat. 8000 München Nees Method for cultivating matrix-bound biological cell systems and device for carrying out the method
US4307965A (en) * 1980-05-30 1981-12-29 Innovative Medical Systems Corp. Mixing apparatus
JPS61138522A (en) * 1984-12-11 1986-06-26 Matsushita Electric Ind Co Ltd Mixing machine
JPS62198334A (en) * 1986-02-26 1987-09-02 カネボウ株式会社 Tissue culture of plant
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
GB9112836D0 (en) * 1991-06-14 1991-07-31 Medical Res Council Production of monoclonal antibodies
DE4229334C2 (en) * 1992-09-02 1995-12-21 Heraeus Instr Gmbh Culture flask similar to a roller bottle for cell cultures
US5744102A (en) * 1996-01-30 1998-04-28 Chugai Biopharmaceuticals, Inc. Solid phase synthesizer
DE19612967A1 (en) * 1996-04-01 1997-10-02 Behringwerke Ag Process for the propagation of influenza viruses in cell culture, and the influenza viruses obtainable by the process

Also Published As

Publication number Publication date
WO1999023204A1 (en) 1999-05-14
EP1027425A1 (en) 2000-08-16
EP1027425A4 (en) 2006-04-19
AU737705B2 (en) 2001-08-30
CA2308111C (en) 2010-12-21
JP2001521736A (en) 2001-11-13
AU1201599A (en) 1999-05-24

Similar Documents

Publication Publication Date Title
Shimizu et al. Stereoselective reduction of ethyl 4-chloro-3-oxobutanoate by a microbial aldehyde reductase in an organic solvent-water diphasic system
Morikawa et al. Continuous production of bacitracin by immobilized living whole cells of Bacillus sp.
Yamamoto et al. Continuous production of l‐alanine using Pseudomonas dacunhae immobilized with carrageenan
Gross et al. Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis
Ohlson et al. Steroid transformation by activated living immobilized Arthrobacter simplex cells
CA2207368A1 (en) Tower reactors for bioconversion of lignocellulosic material
GR900100566A (en) Production of zeaxanthin and zeaxanthin-containing compositions
DE69329154D1 (en) INCREASED PRODUCTION OF TAXOL AND TAXANES BY MEANS OF TAXUS-SPECIFIC CELL CULTURES
CN206359512U (en) solid state fermentation drum
Wehtje et al. Lipases have similar water activity profiles in different reactions
Kometani et al. Large-scale preparation of (S)-ethyl 3-hydroxybutanoate with a high enantiomeric excess through baker's yeast-mediated bioreduction
US5256570A (en) Bioreactor configured for various permeable cell supports and culture media
Raj et al. Rhodococcus rhodochrous PA-34: a potential biocatalyst for acrylamide synthesis
Norouzian et al. Immobilization of whole cell penicillin G acylase in open pore gelatin matrix
US20030054546A1 (en) Biocatalyst chamber encapsulation system for bioremediation and fermentation
Hung et al. Effects of ultraviolet light irradiation in biotreatment of organophosphates
Park et al. Itaconic acid production using an air-lift bioreactor in repeated batch culture of Aspergillus terreus
Kataoka et al. Novel enzymatic production of D-(–)-pantoyl lactone through the stereospecific reduction of ketopantoic acid
Gaikar et al. Selective reverse micellar extraction of penicillin acylase from E coli
RU2077588C1 (en) Method of acrylamide preparing
Ishimura et al. Hydrolysis of penicillin G by combination of immobilized penicillin acylase and electrodialysis
Deshusses et al. Innovative bioreactors
Jianguo et al. Studies of poly (vinyl acetate-co-diviyl benzene) beads as a carrier for the immobilization of penicillin acylase and the kinetics of immobilized penicillin acylase
CN203295262U (en) Biological drum reactor
Meusel et al. Biodegradation of dichloroacetic acid by freely suspended and adsorptive immobilized Xanthobacter autotrophicus GJ10 in soil

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20131029