CA2309000A1 - Systeme de transposon a base de tc1 - Google Patents
Systeme de transposon a base de tc1 Download PDFInfo
- Publication number
- CA2309000A1 CA2309000A1 CA002309000A CA2309000A CA2309000A1 CA 2309000 A1 CA2309000 A1 CA 2309000A1 CA 002309000 A CA002309000 A CA 002309000A CA 2309000 A CA2309000 A CA 2309000A CA 2309000 A1 CA2309000 A1 CA 2309000A1
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- Prior art keywords
- nucleic acid
- cell
- sequence
- coding sequence
- transposase
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
- C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La présente invention concerne des vecteurs de transposon codant pour des pièges à gènes et des pièges à régions de commande d'expression. La présente invention concerne également des vecteurs dicistroniques. Selon certains modes de réalisation, l'invention concerne, en outre, des sites d'entrée de ribosome interne.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6530397P | 1997-11-13 | 1997-11-13 | |
| US60/065,303 | 1997-11-13 | ||
| PCT/US1998/024348 WO1999025817A2 (fr) | 1997-11-13 | 1998-11-13 | Systeme de transposon a base d'adn permettant d'introduire un acide nucleique dans l'adn d'une cellule |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2309000A1 true CA2309000A1 (fr) | 1999-05-27 |
Family
ID=22061760
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002309000A Abandoned CA2309000A1 (fr) | 1997-11-13 | 1998-11-13 | Systeme de transposon a base de tc1 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1034258A2 (fr) |
| JP (1) | JP2001523450A (fr) |
| AU (1) | AU1410399A (fr) |
| CA (1) | CA2309000A1 (fr) |
| WO (1) | WO1999025817A2 (fr) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE466948T1 (de) | 1997-03-11 | 2010-05-15 | Univ Minnesota | Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle |
| US7160682B2 (en) | 1998-11-13 | 2007-01-09 | Regents Of The University Of Minnesota | Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell |
| JP2002543792A (ja) * | 1999-05-11 | 2002-12-24 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | ベクターによるトランスポゾン配列の供給及び組込み |
| WO2001029205A2 (fr) * | 1999-10-19 | 2001-04-26 | Minos Biosystems Limited | Procede de manipulation genetique |
| WO2001030965A2 (fr) | 1999-10-28 | 2001-05-03 | The Board Of Trustees Of The Leland Stanford Junior University | Methodes de transfert genique in vivo a l'aide d'un systeme de transposon «sleeping beauty» |
| CA2398790A1 (fr) | 2000-01-28 | 2001-08-02 | The Scripps Research Institute | Procedes pour identifier des elements regulateurs transcriptionnels et translationnels synthetiques, et compositions correspondantes |
| DE10120829A1 (de) * | 2000-04-27 | 2001-12-20 | Max Delbrueck Centrum | Sleeping Beauty, ein Transposonvektor mit breitem Wirtsbereich für die genetische Transformation bei Wirbeltieren |
| WO2001089579A2 (fr) * | 2000-05-19 | 2001-11-29 | Regents Of The University Of Minnesota | Composition pour l'introduction de composes dans des cellules |
| WO2003089618A2 (fr) | 2002-04-22 | 2003-10-30 | Regents Of The University Of Minnesota | Systeme de transposons, et procedes d'utilisation |
| DE10224242A1 (de) * | 2002-05-29 | 2003-12-11 | Max Delbrueck Centrum | Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren |
| US7985739B2 (en) | 2003-06-04 | 2011-07-26 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced sleeping beauty transposon system and methods for using the same |
| US7919583B2 (en) | 2005-08-08 | 2011-04-05 | Discovery Genomics, Inc. | Integration-site directed vector systems |
| EP2160461B1 (fr) | 2007-07-04 | 2012-08-08 | Max-Delbrück-Centrum für Molekulare Medizin (MDC) | Variantes hyperactives de protéine transposase du système de transposon sleeping beauty |
| WO2011153440A2 (fr) | 2010-06-04 | 2011-12-08 | Regents Of The University Of Minnesota | Système de mutagenèse par transposons et ses méthodes d'utilisation |
| CA2936564C (fr) | 2014-01-07 | 2022-10-18 | Fundacio Privada Institut De Medicina Predictiva I Personalitzada Del Cancer | Methodes de generation de librairies d'adn a double brin et methodes de sequencage destinees a l'identification de cytosines methylatees |
| WO2019010551A1 (fr) * | 2017-07-10 | 2019-01-17 | Instituto De Biologia Molecular Do Paraná - Ibmp | Plateforme génétique pour la surexpression hétérologue associée à la sélection de cellules hautement productrices de protéines |
| SG11202000606TA (en) | 2017-07-29 | 2020-02-27 | Juno Therapeutics Inc | Reagents for expanding cells expressing recombinant receptors |
| US12258580B2 (en) | 2017-11-01 | 2025-03-25 | Juno Therapeutics, Inc. | Process for generating therapeutic compositions of engineered cells |
| EP3704229B1 (fr) | 2017-11-01 | 2023-12-20 | Juno Therapeutics, Inc. | Procédé pour la production d'une composition de lymphocytes t |
| CN119193493A (zh) | 2017-12-08 | 2024-12-27 | 朱诺治疗学股份有限公司 | 生产工程化t细胞组合物的过程 |
| JP2022554348A (ja) | 2019-11-05 | 2022-12-28 | ジュノー セラピューティクス インコーポレイテッド | 治療用ｔ細胞組成物の属性を決定する方法 |
| EP4127184A4 (fr) * | 2020-03-30 | 2024-04-24 | Institute Of Zoology, Chinese Academy Of Sciences | Systèmes de transposon d'adn actif et leurs procédés d'utilisation |
| KR20230024283A (ko) | 2020-05-13 | 2023-02-20 | 주노 쎄러퓨티크스 인코퍼레이티드 | 임상 반응과 관련된 특징을 식별하는 방법 및 이의 용도 |
| AU2022241654A1 (en) | 2021-03-22 | 2023-09-28 | Juno Therapeutics, Inc. | Methods of determining potency of a therapeutic cell composition |
| WO2023122716A1 (fr) | 2021-12-22 | 2023-06-29 | Vanderbilt University | Transpososomes de nouvelle génération |
| US20250345432A1 (en) | 2022-05-25 | 2025-11-13 | Celgene Corporation | Method for predicting response to a t cell therapy |
| KR20250013199A (ko) | 2022-05-27 | 2025-01-31 | 카이트 파마 인코포레이티드 | 세포 요법 작제물의 비-바이러스 전달 |
| WO2024220588A1 (fr) | 2023-04-18 | 2024-10-24 | Juno Therapeutics, Inc. | Essai cytotoxique pour évaluer la puissance de compositions de cellules thérapeutiques |
| KR20260026101A (ko) | 2023-05-23 | 2026-02-25 | 주노 쎄러퓨티크스 인코퍼레이티드 | T 세포의 활성화 마커 및 t 세포 활성화를 평가하는 방법 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001503962A (ja) * | 1996-02-09 | 2001-03-27 | ヘット ネーデルランツ カンケル インスティチュート | 細胞のゲノム中に組込まれる付加核酸物質を細胞に付与するためのベクターおよび方法 |
| ATE466948T1 (de) * | 1997-03-11 | 2010-05-15 | Univ Minnesota | Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle |
-
1998
- 1998-11-13 CA CA002309000A patent/CA2309000A1/fr not_active Abandoned
- 1998-11-13 AU AU14103/99A patent/AU1410399A/en not_active Abandoned
- 1998-11-13 WO PCT/US1998/024348 patent/WO1999025817A2/fr not_active Ceased
- 1998-11-13 JP JP2000521183A patent/JP2001523450A/ja not_active Withdrawn
- 1998-11-13 EP EP98957974A patent/EP1034258A2/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999025817A9 (fr) | 1999-09-10 |
| WO1999025817A2 (fr) | 1999-05-27 |
| AU1410399A (en) | 1999-06-07 |
| WO1999025817A3 (fr) | 1999-08-05 |
| EP1034258A2 (fr) | 2000-09-13 |
| JP2001523450A (ja) | 2001-11-27 |
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