CA2309345C - High throughput method for functionally classifying proteins identified using a genomics approach - Google Patents

High throughput method for functionally classifying proteins identified using a genomics approach Download PDF

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Publication number
CA2309345C
CA2309345C CA002309345A CA2309345A CA2309345C CA 2309345 C CA2309345 C CA 2309345C CA 002309345 A CA002309345 A CA 002309345A CA 2309345 A CA2309345 A CA 2309345A CA 2309345 C CA2309345 C CA 2309345C
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CA
Canada
Prior art keywords
protein
multiplicity
target protein
molecules
containers
Prior art date
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Expired - Lifetime
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CA002309345A
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English (en)
French (fr)
Other versions
CA2309345A1 (en
Inventor
Michael W. Pantoliano
Francis R. Salemme
Jr. Carver Theodore E.
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Life Technologies Corp
Johnson and Johnson Pharmaceutical Research and Development LLC
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Publication of CA2309345A1 publication Critical patent/CA2309345A1/en
Application granted granted Critical
Publication of CA2309345C publication Critical patent/CA2309345C/en
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96444—Factor X (3.4.21.6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
    • G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002309345A 1997-11-12 1998-11-12 High throughput method for functionally classifying proteins identified using a genomics approach Expired - Lifetime CA2309345C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6512997P 1997-11-12 1997-11-12
US60/065,129 1997-11-12
PCT/US1998/024035 WO1999024050A1 (en) 1997-11-12 1998-11-12 High throughput method for functionally classifying proteins identified using a genomics approach

Publications (2)

Publication Number Publication Date
CA2309345A1 CA2309345A1 (en) 1999-05-20
CA2309345C true CA2309345C (en) 2007-01-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002309345A Expired - Lifetime CA2309345C (en) 1997-11-12 1998-11-12 High throughput method for functionally classifying proteins identified using a genomics approach

Country Status (15)

Country Link
US (2) US20010003648A1 (de)
EP (1) EP1030678B1 (de)
JP (1) JP2002514571A (de)
KR (2) KR20030040425A (de)
AT (1) ATE335498T1 (de)
AU (1) AU750501B2 (de)
CA (1) CA2309345C (de)
DE (1) DE69835531T2 (de)
DK (1) DK1030678T3 (de)
ES (1) ES2273442T3 (de)
HU (1) HU225767B1 (de)
IL (1) IL136046A0 (de)
NZ (1) NZ504483A (de)
PL (1) PL194483B1 (de)
WO (1) WO1999024050A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997009342A1 (en) * 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
IL136046A0 (en) 1997-11-12 2001-05-20 Dimensional Pharm Inc Methods for classifying proteins
US6569631B1 (en) * 1998-11-12 2003-05-27 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes
JP2004537047A (ja) * 2001-06-14 2004-12-09 アナディーズ　ファーマスーティカルズ，アイエヌシー． 標的分子のリガンドをスクリーニングする方法
WO2003025528A2 (en) * 2001-09-18 2003-03-27 Energetic Geonomics Corporation A high throughput energy array
US20030170810A1 (en) * 2001-09-18 2003-09-11 Masoud Vedadi Methods and apparatuses for purification
GB0125436D0 (en) * 2001-10-23 2001-12-12 Deltadot Ltd Analysis of temperature-dependent molecular configurations
WO2003061570A2 (en) * 2002-01-16 2003-07-31 Zyomyx, Inc. Engineered binding proteins
CA2414570A1 (en) * 2002-01-24 2003-04-03 Ecopia Biosciences Inc. Method, system, and knowledge repository for identifying a secondary metabolite from a microorganism
US20050079526A1 (en) * 2002-02-20 2005-04-14 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing refolding and aggregation of biological molecules
CA2477021A1 (en) * 2002-02-20 2003-08-28 Affinium Pharmaceuticals, Inc. Methods and apparatuses for characterizing stability of biological molecules
WO2003087834A2 (en) * 2002-04-08 2003-10-23 Affinium Pharmaceuticals, Inc. High throughput analysis of recombinant proteins in multi-well plates
US20040121445A1 (en) * 2002-07-31 2004-06-24 Fabien Marino Cell cultures
DE102004017039A1 (de) * 2004-04-02 2005-11-03 Rwth Aachen Verfahren und Vorrichtung zur Erfassung von Prozessparametern von Reaktionsflüssigkeiten in mehreren geschüttelten Mikroreaktoren
WO2009055050A1 (en) * 2007-10-25 2009-04-30 Canon U.S. Life Sciences, Inc High-resolution melting analysis
US8993714B2 (en) * 2007-10-26 2015-03-31 Imiplex Llc Streptavidin macromolecular adaptor and complexes thereof
US9102526B2 (en) 2008-08-12 2015-08-11 Imiplex Llc Node polypeptides for nanostructure assembly
WO2010109204A1 (en) * 2009-03-26 2010-09-30 Ucb Pharma S.A. Thermofluor method
WO2010132363A1 (en) 2009-05-11 2010-11-18 Imiplex Llc Method of protein nanostructure fabrication
GB201106548D0 (en) * 2011-04-18 2011-06-01 Evitraproteoma Ab A method for determining ligand binding to a target protein using a thermal shift assahy
US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
WO2013034160A1 (en) * 2011-09-06 2013-03-14 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Methods for analyzing biological macromolecular complexes and use thereof
US9568478B2 (en) 2012-01-27 2017-02-14 Life Technologies Corporation Protein melt analysis using dipyrrometheneboron difluoride compounds
US10024863B2 (en) 2013-03-15 2018-07-17 Life Technologies Corporation Methods for dye selection for protein melt temperature determinations
GB2524519B (en) * 2014-03-25 2019-11-06 Pelago Bioscience AB Methods for identifying a biomarker indicative of a reduced drug response using a thermal shift assay
WO2016140990A1 (en) * 2015-03-01 2016-09-09 Board Of Regents, The University Of Texas System Apparatuses and methods for pathogen detection using microfluidic biochips
US11001872B2 (en) * 2015-09-22 2021-05-11 Shawn Clark Designing customized protein-specific buffer system
US11466309B2 (en) 2018-10-12 2022-10-11 The Board Of Trustees Of The University Of Arkansas Mechanically-strained oligonucleotide constructs and methods of using the same
CN121039476A (zh) * 2023-04-13 2025-11-28 赛默卡普实验室有限公司 多个样品的同时热测量

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4626684A (en) 1983-07-13 1986-12-02 Landa Isaac J Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples
US4580895A (en) 1983-10-28 1986-04-08 Dynatech Laboratories, Incorporated Sample-scanning photometer
US4628026A (en) 1983-11-15 1986-12-09 Dietlind Gardell Method and apparatus for automated double fluorochromization analysis in lymphocytotoxicity testing
US5096807A (en) 1985-03-06 1992-03-17 Murex Corporation Imaging immunoassay detection system with background compensation and its use
JPS61241639A (ja) 1985-04-19 1986-10-27 Hitachi Ltd 反応試料分析装置
DE3683573D1 (de) 1985-06-26 1992-03-05 Japan Tectron Instr Corp Automatischer analysenapparat.
US4859609A (en) 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4880750A (en) * 1987-07-09 1989-11-14 Miragen, Inc. Individual-specific antibody identification methods
US5217869A (en) 1987-10-13 1993-06-08 Terrapin Technologies, Inc. Method to produce immunodiagnostic reagents
US5338659A (en) 1991-04-02 1994-08-16 Terrapin Technologies, Inc. Method for determining analyte concentration by cross-reactivity profiling
US5133866A (en) 1988-03-24 1992-07-28 Terrapin Technologies, Inc. Method to identify analyte-bending ligands
US5300425A (en) 1987-10-13 1994-04-05 Terrapin Technologies, Inc. Method to produce immunodiagnostic reagents
US4963263A (en) 1988-03-24 1990-10-16 Terrapin Technologies, Inc. Method of identity analyte-binding peptides
US5340474A (en) 1988-03-24 1994-08-23 Terrapin Technologies, Inc. Panels of analyte-binding ligands
US5409611A (en) 1988-03-24 1995-04-25 Terrapin Technoogies, Inc. Method to identify analyte-binding ligands
JP2656564B2 (ja) 1988-08-26 1997-09-24 株式会社日立製作所 免疫分析方法
US5325295A (en) 1989-05-04 1994-06-28 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Adaptation of microtiter plate technology to measurement of platelet aggregation
HUT69771A (en) 1990-08-17 1995-09-28 Univ Boston Proteases causing abnormal degradation of amyloid beta-proteine precursors
US5506097A (en) 1990-08-24 1996-04-09 President And Fellows Of Harvard College Method for inhibiting β-protein enzymatic activity
US5200504A (en) 1990-10-02 1993-04-06 The Scripps Research Institute Metallopeptides having stabilized secondary structures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE4123817C2 (de) 1991-07-18 1994-06-09 Berthold Lab Prof Dr Strahlungsmeßgerät, insbesondere zur Messung der Lumineszenz
WO1993014781A1 (en) 1992-01-24 1993-08-05 The Regents Of The University Of California Novel peptides and method for altering the activity of allosteric proteins
EP0592624B1 (de) 1992-03-09 2001-09-19 Accumed International Inc. Diagnostische mikrobiologische testvorrichtung und verfahren
CA2093481A1 (en) 1992-04-30 1993-10-31 Gottlieb Schacher Processing station for carrying out fluorescence polarization measurements in an analyzer
US5255976A (en) 1992-07-10 1993-10-26 Vertex Pharmaceuticals Incorporated Temperature gradient calorimeter
US5314825A (en) 1992-07-16 1994-05-24 Schiapparelli Biosystems, Inc. Chemical analyzer
US5585275A (en) 1992-09-02 1996-12-17 Arris Pharmaceutical Corporation Pilot apparatus for peptide synthesis and screening
US5355215A (en) 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
CA2115900A1 (en) 1993-02-22 1994-08-23 Gerald W. Becker Pharmaceutical screens and antibodies
US5383023A (en) 1993-03-01 1995-01-17 Walleczek; Jan Method and apparatus for performing dual-beam dual-wavelength fluorescence spectrophotometric evaluation of a biological specimen
US5356784A (en) 1993-03-30 1994-10-18 Terrapin Technologies, Inc. Determination of concentration by affinity titration
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5585277A (en) * 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
JP3598123B2 (ja) 1993-07-15 2004-12-08 浜松ホトニクス株式会社 核酸の変性検出装置
US5436718A (en) 1993-07-30 1995-07-25 Biolumin Corporation Mutli-functional photometer with movable linkage for routing optical fibers
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5525300A (en) 1993-10-20 1996-06-11 Stratagene Thermal cycler including a temperature gradient block
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
ATE236401T1 (de) 1994-01-06 2003-04-15 Telik Inc Surrogate für bindemoleküle und verbesserte referenzpanele
US5587293A (en) 1994-01-06 1996-12-24 Terrapin Technologies, Inc. Method to identify binding partners
US5935803A (en) 1994-02-01 1999-08-10 Terrapin Technologies, Inc. Methods to identify immunomodulators using cognate interaction of PKC-theta
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5557398A (en) 1994-04-15 1996-09-17 Molecular Devices Corporation Photometric device
US5463564A (en) 1994-09-16 1995-10-31 3-Dimensional Pharmaceuticals, Inc. System and method of automatically generating chemical compounds with desired properties
US5589351A (en) 1994-12-06 1996-12-31 Nps Pharmaceuticals, Inc. Fluorescence detection apparatus
AU5132096A (en) 1995-01-30 1996-08-21 Terrapin Technologies, Inc. Glubodies - multiplicities of proteins capable of binding a variety of small molecules
WO1997009342A1 (en) 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
US5633762A (en) * 1995-10-23 1997-05-27 Motorola Dual image manifestation apparatus with integrated electro-optical package
CA2184195C (en) 1995-10-25 2002-04-16 Andrew Pakula Screening method for identifying ligands for target proteins
JP2000502440A (ja) * 1995-12-07 2000-02-29 スクリプトジェン・ファーマスーティカルズ，インコーポレイテッド リガンドを識別するための蛍光に基づくスクリーニング方法
HUP9902418A3 (en) 1996-05-09 2001-10-29 Dimensional Pharm Inc Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
DE69735411T2 (de) 1996-10-09 2006-09-07 Symyx Technologies, Inc., Santa Clara Infrarot-spektroskopie und abbildung von bibliotheken
US6331392B1 (en) 1997-03-05 2001-12-18 Anadys Pharmaceutical, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
IL136046A0 (en) 1997-11-12 2001-05-20 Dimensional Pharm Inc Methods for classifying proteins
US6376180B1 (en) 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions

Also Published As

Publication number Publication date
US7122321B2 (en) 2006-10-17
EP1030678A1 (de) 2000-08-30
DK1030678T3 (da) 2006-12-11
IL136046A0 (en) 2001-05-20
KR20010032048A (ko) 2001-04-16
HUP0100037A3 (en) 2003-08-28
WO1999024050A9 (en) 1999-08-12
PL340449A1 (en) 2001-02-12
EP1030678A4 (de) 2002-10-09
KR100406310B1 (ko) 2003-11-19
AU750501B2 (en) 2002-07-18
HU225767B1 (en) 2007-08-28
NZ504483A (en) 2002-11-26
US20010003648A1 (en) 2001-06-14
US20020168686A1 (en) 2002-11-14
KR20030040425A (ko) 2003-05-22
AU1398099A (en) 1999-05-31
DE69835531D1 (de) 2006-09-21
HUP0100037A2 (hu) 2001-05-28
EP1030678B1 (de) 2006-08-09
PL194483B1 (pl) 2007-06-29
DE69835531T2 (de) 2007-04-05
ATE335498T1 (de) 2006-09-15
ES2273442T3 (es) 2007-05-01
CA2309345A1 (en) 1999-05-20
JP2002514571A (ja) 2002-05-21
WO1999024050A1 (en) 1999-05-20

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