CA2310510A1 - Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules - Google Patents

Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules Download PDF

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Publication number
CA2310510A1
CA2310510A1 CA002310510A CA2310510A CA2310510A1 CA 2310510 A1 CA2310510 A1 CA 2310510A1 CA 002310510 A CA002310510 A CA 002310510A CA 2310510 A CA2310510 A CA 2310510A CA 2310510 A1 CA2310510 A1 CA 2310510A1
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CA
Canada
Prior art keywords
gene
genes
functional
identifying
egss
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002310510A
Other languages
English (en)
Other versions
CA2310510C (fr
Inventor
Timothy W. Nilsen
Hugh D. Robertson
Thomas J. Kindt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/976,220 external-priority patent/US6013447A/en
Application filed by Individual filed Critical Individual
Publication of CA2310510A1 publication Critical patent/CA2310510A1/fr
Application granted granted Critical
Publication of CA2310510C publication Critical patent/CA2310510C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/126—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes involving RNAse P

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002310510A 1997-11-21 1998-11-20 Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules Expired - Fee Related CA2310510C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/976,220 US6013447A (en) 1997-11-21 1997-11-21 Random intracellular method for obtaining optimally active nucleic acid molecules
US976,220 1997-11-21
US7985198P 1998-03-30 1998-03-30
US60/079,851 1998-03-30
PCT/US1998/024854 WO1999027135A2 (fr) 1997-11-21 1998-11-20 Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules

Publications (2)

Publication Number Publication Date
CA2310510A1 true CA2310510A1 (fr) 1999-06-03
CA2310510C CA2310510C (fr) 2007-04-17

Family

ID=26762503

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002310510A Expired - Fee Related CA2310510C (fr) 1997-11-21 1998-11-20 Procede d'identification et d'inhibition de molecules fonctionnelles d'acide nucleique dans des cellules

Country Status (5)

Country Link
EP (1) EP1032707A2 (fr)
JP (1) JP2001524317A (fr)
AU (1) AU732321B2 (fr)
CA (1) CA2310510C (fr)
WO (1) WO1999027135A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028508A1 (fr) * 1997-12-04 1999-06-10 Smithkline Beecham Corporation Procede mettant en oeuvre des sequences antisens d'expression pour produire des cellules mutantes exprimees de maniere conditionnelle
AU4186600A (en) * 1999-03-31 2000-10-16 Rosetta Inpharmatics, Inc. Essential genes of yeast as targets for antifungal agents, herbicides, insecticides and anti-proliferation drugs
GB9912965D0 (en) * 1999-06-03 1999-08-04 Oxford Biomedica Ltd In vivo selection method
GB9929744D0 (en) * 1999-12-17 2000-02-09 Univ Nottingham Modifying micro-organisms
EP1248856A2 (fr) * 1999-12-23 2002-10-16 Xantos Biomedicine AG Procede d'echantillonnage pour acides nucleiques
AU2008202554C1 (en) * 2001-02-23 2011-07-07 Dsm Ip Assets B.V. Novel genes encoding novel proteolytic enzymes
US20130244905A1 (en) * 2010-07-06 2013-09-19 Ed Grabczyk Reporter for RNA Polymerase II Termination

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5168053A (en) * 1989-03-24 1992-12-01 Yale University Cleavage of targeted RNA by RNAase P
JP3015463B2 (ja) * 1992-04-28 2000-03-06 エール ユニバーシティ 真核リボヌクレアーゼｐを用いるｒｎａの標的化切断および外部ガイド配列
WO1994013791A1 (fr) * 1992-12-04 1994-06-23 Innovir Laboratories, Inc. Acide nucleique regulable a usage therapeutique et procedes d'utilisation associes
AU4961996A (en) * 1994-12-14 1996-07-03 Innovir Laboratories, Inc. Ribozyme-mediated inactivation of leukemia-associated rna
EP0850318A1 (fr) * 1995-09-13 1998-07-01 Chiron Corporation Procede et produit d'assemblage pour la recherche d'inhibiteurs d'activation de la transcription
US5877162A (en) * 1996-03-14 1999-03-02 Innovir Laboratories, Inc. Short external guide sequences
US5976874A (en) * 1996-08-16 1999-11-02 Yale University Phenotypic conversion of drug-resistant bacteria to drug-sensitivity
CA2278734A1 (fr) * 1997-01-23 1998-07-30 Immusol Incorporated Analyse fonctionnelle et decouverte de genes a l'aide de banques de vecteurs de genes de ribozymes specifiques a une cible ou bien rendues aleatoires

Also Published As

Publication number Publication date
AU732321B2 (en) 2001-04-12
JP2001524317A (ja) 2001-12-04
EP1032707A2 (fr) 2000-09-06
WO1999027135A3 (fr) 1999-07-29
AU1532399A (en) 1999-06-15
WO1999027135A2 (fr) 1999-06-03
CA2310510C (fr) 2007-04-17

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Effective date: 20141120