CA2311193C - Methode de production de matieres proteiniques vegetales ultrapures - Google Patents

Methode de production de matieres proteiniques vegetales ultrapures Download PDF

Info

Publication number: CA2311193C
Authority: CA; Canada
Prior art keywords: slurry; protein material; vegetable protein; treated; effective
Prior art date: 2000-06-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA 2311193

Other languages

English (en)

Other versions

CA2311193A1 (fr

Inventor

Theodore M. Wong

David A. Singer

Santa H. Lin (Deceased)

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Solae LLC

Original Assignee

Protein Technologies International Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-06-12

Filing date

2000-06-12

Publication date

2004-08-24

2000-06-12 Application filed by Protein Technologies International Inc filed Critical Protein Technologies International Inc

2000-06-12 Priority to CA 2311193 priority Critical patent/CA2311193C/fr

2001-12-12 Publication of CA2311193A1 publication Critical patent/CA2311193A1/fr

2004-08-24 Application granted granted Critical

2004-08-24 Publication of CA2311193C publication Critical patent/CA2311193C/fr

2020-06-12 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

239000000463 material Substances 0.000 title claims abstract description 194
108010082495 Dietary Plant Proteins Proteins 0.000 title claims abstract description 129
238000004519 manufacturing process Methods 0.000 title claims description 5
239000002002 slurry Substances 0.000 claims abstract description 193
IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims abstract description 154
235000002949 phytic acid Nutrition 0.000 claims abstract description 153
102000004169 proteins and genes Human genes 0.000 claims abstract description 124
108090000623 proteins and genes Proteins 0.000 claims abstract description 124
229920002477 rna polymer Polymers 0.000 claims abstract description 115
238000000034 method Methods 0.000 claims abstract description 106
102000004190 Enzymes Human genes 0.000 claims abstract description 103
108090000790 Enzymes Proteins 0.000 claims abstract description 103
229940088598 enzyme Drugs 0.000 claims abstract description 103
IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims abstract description 87
229940068041 phytic acid Drugs 0.000 claims abstract description 86
239000000467 phytic acid Substances 0.000 claims abstract description 86
108010051457 Acid Phosphatase Proteins 0.000 claims abstract description 72
102000013563 Acid Phosphatase Human genes 0.000 claims abstract description 72
108010011619 6-Phytase Proteins 0.000 claims abstract description 48
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 claims description 61
238000002360 preparation method Methods 0.000 claims description 57
229940071440 soy protein isolate Drugs 0.000 claims description 39
230000000694 effects Effects 0.000 claims description 33
239000007787 solid Substances 0.000 claims description 33
229940085127 phytase Drugs 0.000 claims description 30
108091005804 Peptidases Proteins 0.000 claims description 22
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 22
238000005406 washing Methods 0.000 claims description 21
239000012141 concentrate Substances 0.000 claims description 18
108010073771 Soybean Proteins Proteins 0.000 claims description 14
229940001941 soy protein Drugs 0.000 claims description 14
229910052500 inorganic mineral Inorganic materials 0.000 claims description 13
239000011707 mineral Substances 0.000 claims description 13
230000007062 hydrolysis Effects 0.000 claims description 8
238000006460 hydrolysis reaction Methods 0.000 claims description 8
238000001035 drying Methods 0.000 claims description 6
239000000523 sample Substances 0.000 description 31
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 24
239000013068 control sample Substances 0.000 description 20
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 16
KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 16
239000004365 Protease Substances 0.000 description 13
230000007515 enzymatic degradation Effects 0.000 description 12
239000007921 spray Substances 0.000 description 12
235000019419 proteases Nutrition 0.000 description 11
238000010411 cooking Methods 0.000 description 10
239000000203 mixture Substances 0.000 description 10
235000010469 Glycine max Nutrition 0.000 description 9
108010080981 3-phytase Proteins 0.000 description 8
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
229940079919 digestives enzyme preparation Drugs 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
238000004458 analytical method Methods 0.000 description 5
239000007864 aqueous solution Substances 0.000 description 5
230000007071 enzymatic hydrolysis Effects 0.000 description 5
238000006047 enzymatic hydrolysis reaction Methods 0.000 description 5
235000013350 formula milk Nutrition 0.000 description 5
238000010438 heat treatment Methods 0.000 description 5
238000002156 mixing Methods 0.000 description 5
239000011574 phosphorus Substances 0.000 description 5
229910052698 phosphorus Inorganic materials 0.000 description 5
239000011701 zinc Substances 0.000 description 5
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 4
239000002253 acid Substances 0.000 description 4
239000011575 calcium Substances 0.000 description 4
229910052791 calcium Inorganic materials 0.000 description 4
229910052802 copper Inorganic materials 0.000 description 4
239000010949 copper Substances 0.000 description 4
229910052742 iron Inorganic materials 0.000 description 4
239000011591 potassium Substances 0.000 description 4
229910052700 potassium Inorganic materials 0.000 description 4
239000011734 sodium Substances 0.000 description 4
229910052708 sodium Inorganic materials 0.000 description 4
238000001694 spray drying Methods 0.000 description 4
229910052725 zinc Inorganic materials 0.000 description 4
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 3
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 3
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 3
230000015556 catabolic process Effects 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
239000003153 chemical reaction reagent Substances 0.000 description 3
238000006731 degradation reaction Methods 0.000 description 3
230000000593 degrading effect Effects 0.000 description 3
230000002255 enzymatic effect Effects 0.000 description 3
238000001914 filtration Methods 0.000 description 3
239000012535 impurity Substances 0.000 description 3
239000011777 magnesium Substances 0.000 description 3
229910052749 magnesium Inorganic materials 0.000 description 3
WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 3
230000000813 microbial effect Effects 0.000 description 3
239000000047 product Substances 0.000 description 3
239000000243 solution Substances 0.000 description 3
241000228212 Aspergillus Species 0.000 description 2
241000228245 Aspergillus niger Species 0.000 description 2
108010004032 Bromelains Proteins 0.000 description 2
244000068988 Glycine max Species 0.000 description 2
GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
241000235527 Rhizopus Species 0.000 description 2
239000012670 alkaline solution Substances 0.000 description 2
239000002956 ash Substances 0.000 description 2
235000019835 bromelain Nutrition 0.000 description 2
235000014633 carbohydrates Nutrition 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
239000003518 caustics Substances 0.000 description 2
229920002678 cellulose Polymers 0.000 description 2
239000001913 cellulose Substances 0.000 description 2
238000007865 diluting Methods 0.000 description 2
239000000835 fiber Substances 0.000 description 2
230000002538 fungal effect Effects 0.000 description 2
238000011534 incubation Methods 0.000 description 2
239000002198 insoluble material Substances 0.000 description 2
239000011572 manganese Substances 0.000 description 2
235000010755 mineral Nutrition 0.000 description 2
229910017604 nitric acid Inorganic materials 0.000 description 2
235000016709 nutrition Nutrition 0.000 description 2
235000013824 polyphenols Nutrition 0.000 description 2
229940116540 protein supplement Drugs 0.000 description 2
235000005974 protein supplement Nutrition 0.000 description 2
GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
239000007858 starting material Substances 0.000 description 2
235000013311 vegetables Nutrition 0.000 description 2
238000005303 weighing Methods 0.000 description 2
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
240000002791 Brassica napus Species 0.000 description 1
235000004977 Brassica sinapistrum Nutrition 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
244000046052 Phaseolus vulgaris Species 0.000 description 1
235000010582 Pisum sativum Nutrition 0.000 description 1
240000004713 Pisum sativum Species 0.000 description 1
102000006382 Ribonucleases Human genes 0.000 description 1
108010083644 Ribonucleases Proteins 0.000 description 1
LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
229910052770 Uranium Inorganic materials 0.000 description 1
FENRSEGZMITUEF-ATTCVCFYSA-E [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+].OP(=O)([O-])O[C@@H]1[C@@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H](OP(=O)([O-])[O-])[C@H](OP(=O)(O)[O-])[C@H]1OP(=O)([O-])[O-] FENRSEGZMITUEF-ATTCVCFYSA-E 0.000 description 1
239000012445 acidic reagent Substances 0.000 description 1
239000003929 acidic solution Substances 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
239000000908 ammonium hydroxide Substances 0.000 description 1
235000013361 beverage Nutrition 0.000 description 1
AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
239000000920 calcium hydroxide Substances 0.000 description 1
229910001861 calcium hydroxide Inorganic materials 0.000 description 1
150000001768 cations Chemical class 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000010908 decantation Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
235000013325 dietary fiber Nutrition 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000002657 fibrous material Substances 0.000 description 1
235000013305 food Nutrition 0.000 description 1
235000020256 human milk Nutrition 0.000 description 1
210000004251 human milk Anatomy 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
229910052816 inorganic phosphate Inorganic materials 0.000 description 1
238000002386 leaching Methods 0.000 description 1
235000021374 legumes Nutrition 0.000 description 1
229910052748 manganese Inorganic materials 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000000926 separation method Methods 0.000 description 1
229940083982 sodium phytate Drugs 0.000 description 1
235000010265 sodium sulphite Nutrition 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
239000005418 vegetable material Substances 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Nutrition Science (AREA)
Biochemistry (AREA)
Engineering & Computer Science (AREA)
Food Science & Technology (AREA)
Polymers & Plastics (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

CA 2311193 2000-06-12 2000-06-12 Methode de production de matieres proteiniques vegetales ultrapures Expired - Fee Related CA2311193C (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA 2311193 CA2311193C (fr)	2000-06-12	2000-06-12	Methode de production de matieres proteiniques vegetales ultrapures

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA 2311193 CA2311193C (fr)	2000-06-12	2000-06-12	Methode de production de matieres proteiniques vegetales ultrapures

Publications (2)

Publication Number	Publication Date
CA2311193A1 CA2311193A1 (fr)	2001-12-12
CA2311193C true CA2311193C (fr)	2004-08-24

Family

ID=4166449

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2311193 Expired - Fee Related CA2311193C (fr)	2000-06-12	2000-06-12	Methode de production de matieres proteiniques vegetales ultrapures

Country Status (1)

Country	Link
CA (1)	CA2311193C (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN103431159A (zh) *	2013-05-16	2013-12-11	华南理工大学	一种低值酸的大豆分离蛋白及其制备方法

2000
- 2000-06-12 CA CA 2311193 patent/CA2311193C/fr not_active Expired - Fee Related

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN103431159A (zh) *	2013-05-16	2013-12-11	华南理工大学	一种低值酸的大豆分离蛋白及其制备方法
CN103431159B (zh) *	2013-05-16	2014-10-08	华南理工大学	一种低值酸的大豆分离蛋白及其制备方法

Also Published As

Publication number	Publication date
CA2311193A1 (fr)	2001-12-12

Legal Events

Date	Code	Title	Description
2000-08-15	EEER	Examination request
2010-06-14	MKLA	Lapsed

Publication	Publication Date	Title
US20010018197A1 (en)	2001-08-30	Method for producing ultrapure vegetable protein materials
US5989600A (en)	1999-11-23	Method for improving the solubility of vegetable proteins
JP5125514B2 (ja)	2013-01-23	大豆ペプチド混合物の製造法
EP0380343A2 (fr)	1990-08-01	Procédé de production d'isolats et concentrés de protéine de soja sans phytate ou à faible teneur en phytate
NO754410L (fr)	1976-07-05
JP5682697B1 (ja)	2015-03-11	植物性分離蛋白およびその製造法
US20090011083A1 (en)	2009-01-08	Modified Vegetable Protein Having Low Levels of Phytic Acid, Isoflavones and Ash
US4375431A (en)	1983-03-01	Aluminum treated proteins
EP1181869B1 (fr)	2005-10-26	Procédé de préparation d'un matériau protéique végétal purifié à teneur réduite en acides ribonucléiques
EP1482810A2 (fr)	2004-12-08	Concentre de proteine de soja a faible teneur en oligosaccharides non digestibles et son procede de production
CA2311193C (fr)	2004-08-24	Methode de production de matieres proteiniques vegetales ultrapures
US20020127288A1 (en)	2002-09-12	Method for producing ultrapure vegetable protein materials
JP3813055B2 (ja)	2006-08-23	高純度植物タンパク材料の製造方法
US20060159826A1 (en)	2006-07-20	Soy protein for infant formula
US7112424B2 (en)	2006-09-26	Process for the preparation of protein hydrolysate from legumes
US20020123090A1 (en)	2002-09-05	Ultrapure vegetable protein materials
AU750447B2 (en)	2002-07-18	Method for producing ultrapure vegetable protein materials
ZA200306957B (en)	2004-07-28	Process for preparation of protein hydrolysate from soy flour.
KR100703637B1 (ko)	2007-04-05	초정제 식물성 단백질 물질의 제조 방법
JP2000300185A (ja)	2000-10-31	大豆蛋白の製造方法
MXPA00006376A (es)	2002-01-10	Metodo para producir materiales ultrapuros de proteina vegetal.
RU2203555C2 (ru)	2003-05-10	Способ получения ультрачистого растительного белкового материала (варианты)
CN1334020A (zh)	2002-02-06	制备超纯植物蛋白材料的方法
JP2015119699A (ja)	2015-07-02	植物性分離蛋白およびその製造法