CA2318522A1 - Genes de biosynthese de la riboflavine extraits de plantes et leur utilisation - Google Patents

Genes de biosynthese de la riboflavine extraits de plantes et leur utilisation Download PDF

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Publication number
CA2318522A1
CA2318522A1 CA002318522A CA2318522A CA2318522A1 CA 2318522 A1 CA2318522 A1 CA 2318522A1 CA 002318522 A CA002318522 A CA 002318522A CA 2318522 A CA2318522 A CA 2318522A CA 2318522 A1 CA2318522 A1 CA 2318522A1
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CA
Canada
Prior art keywords
plant
enzyme
dna molecule
seq
synthase activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002318522A
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English (en)
Inventor
Charles David Guyer
Marie Ann Johnson
Sandra Lynn Volrath
Sandra Alice Brunn
Eric Russell Ward
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Participations AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2318522A1 publication Critical patent/CA2318522A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des gènes de biosynthèse de la riboflavine extraits de plantes, qui comprennent un gène encodant la sous-unité bêta du complexe enzymatique de la riboflavine synthase de la plante (lumazine synthase), et un gène encodant l'enzyme bifonctionnelle GTP cyclohydrolase II/DHBP synthase. L'invention concerne également la production recombinante desdites enzymes biosynthétiques de la riboflavine extraites de plantes dans des hôtes hétérologues, le criblage de produits chimiques au moyen desdites enzymes obtenues par recombinaison pour déceler une activité herbicide, et l'utilisation de produits chimiques herbicides ainsi identifiés pour freiner la croissance d'une végétation non désirée. L'invention concerne en outre des procédés de mise au point d'une tolérance aux herbicides dans des plantes, tissus de plantes, semences de plantes et cellules de plantes, au moyen desdits gènes de biosynthèse de la riboflavine.
CA002318522A 1998-01-30 1999-01-28 Genes de biosynthese de la riboflavine extraits de plantes et leur utilisation Abandoned CA2318522A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10981098P 1998-01-30 1998-01-30
US60/109,810 1998-01-30
PCT/EP1999/000556 WO1999038986A2 (fr) 1998-01-30 1999-01-28 Genes de biosynthese de la riboflavine extraits de plantes et leur utilisation

Publications (1)

Publication Number Publication Date
CA2318522A1 true CA2318522A1 (fr) 1999-08-05

Family

ID=22329685

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002318522A Abandoned CA2318522A1 (fr) 1998-01-30 1999-01-28 Genes de biosynthese de la riboflavine extraits de plantes et leur utilisation

Country Status (17)

Country Link
EP (1) EP1051504A2 (fr)
JP (1) JP2002501753A (fr)
KR (1) KR20010082509A (fr)
CN (1) CN1289369A (fr)
AU (1) AU744487B2 (fr)
BR (1) BR9908213A (fr)
CA (1) CA2318522A1 (fr)
CZ (1) CZ20002750A3 (fr)
HU (1) HUP0101278A3 (fr)
ID (1) ID25910A (fr)
IL (1) IL137303A0 (fr)
IN (1) IN2000CH00209A (fr)
MX (1) MXPA00007432A (fr)
PL (1) PL342805A1 (fr)
TR (1) TR200002193T2 (fr)
WO (1) WO1999038986A2 (fr)
ZA (1) ZA99716B (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010083077A (ko) * 1998-08-12 2001-08-31 추후제출 제초제 선택성 작물을 생성하는 ｄｎａ 재편성
EP1141381A1 (fr) * 1998-12-15 2001-10-10 Adelbert Bacher Procede de detection d'inhibiteurs de biosynthese de la riboflavine
DE19910102B4 (de) * 1999-03-08 2006-06-01 Fischer, Markus, Dr.rer.nat. Proteinkonjugate, Verfahren, Vektoren, Proteine und DNA zu deren Herstellung, deren Verwendung, sowie Arzneimittel und Impfstoffe mit einem Gehalt derselben
DE19942175A1 (de) * 1999-09-03 2001-03-08 Adelbert Bacher Methode zum Auffinden von Inhibitoren der Riboflavinbiosynthese
WO2001079514A2 (fr) * 2000-04-18 2001-10-25 Syngenta Participations Ag Genes d' « acide amine » glyoxylate aminotransferase d'origine vegetale et utilisations de ceux-ci
CN110964769B (zh) * 2019-11-29 2022-11-11 河南巨龙生物工程股份有限公司 一种提高枯草芽孢杆菌发酵生产核黄素收率的方法

Also Published As

Publication number Publication date
KR20010082509A (ko) 2001-08-30
CZ20002750A3 (cs) 2000-11-15
BR9908213A (pt) 2000-11-28
TR200002193T2 (tr) 2001-05-21
MXPA00007432A (en) 2001-02-01
EP1051504A2 (fr) 2000-11-15
ZA99716B (en) 1999-07-30
IN2000CH00209A (fr) 2005-05-20
JP2002501753A (ja) 2002-01-22
AU744487B2 (en) 2002-02-28
AU2720299A (en) 1999-08-16
HUP0101278A3 (en) 2003-04-28
IL137303A0 (en) 2001-07-24
HUP0101278A2 (hu) 2001-08-28
CN1289369A (zh) 2001-03-28
PL342805A1 (en) 2001-07-02
WO1999038986A2 (fr) 1999-08-05
ID25910A (id) 2000-11-09
WO1999038986A3 (fr) 1999-09-23

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Legal Events

Date Code Title Description
FZDE Discontinued