CA2326687A1 - Interconversion de desaturases et d'hydroxylases d'acides gras vegetaux - Google Patents

Interconversion de desaturases et d'hydroxylases d'acides gras vegetaux Download PDF

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Publication number
CA2326687A1
CA2326687A1 CA002326687A CA2326687A CA2326687A1 CA 2326687 A1 CA2326687 A1 CA 2326687A1 CA 002326687 A CA002326687 A CA 002326687A CA 2326687 A CA2326687 A CA 2326687A CA 2326687 A1 CA2326687 A1 CA 2326687A1
Authority
CA
Canada
Prior art keywords
desaturase
amino acid
modified
hydroxylase
oleate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002326687A
Other languages
English (en)
Inventor
Pierre Broun
John Shanklin
Edward J. Whittle
Chris Somerville
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carnegie Institution of Washington
Brookhaven Science Associates LLC
Monsanto Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2326687A1 publication Critical patent/CA2326687A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne un procédé permettant de modifier en hydroxylase d'acyle gras une désaturase d'acyle gras. Ce procédé consiste à identifier et modifier un maximum de quatre radicaux acide aminé qui sont conservés dans des désaturases fonctionnellement équivalentes issues de diverses espèces végétales, mais qui ne sont pas identiques quant aux hydroxylases d'acyles gras présentant une importante ressemblance de séquence d'ensemble avec les désaturases d'acyles gras, et qui catalysent l'hydroxylation au niveau de l'un des radicaux carbone du substrat acyle gras qui est désaturé par la désaturase correspondante. Les modifications consistent en un changement du radical acide aminé qui est identique ou fonctionnellement équivalent au radical acide aminé se trouvant dans l'hydroxylase naturellement présente. L'invention concerne également un procédé semblable visant à modifier en désaturase d'acyle gras une hydroxylase d'acyle gras en modifiant un maximum de sept radicaux acide aminé. L'invention concerne enfin des plantes transgéniques et des produits de telles plantes transgéniques, auquel cas les plantes ont été modifiées pour produire une hydroxylase ou une désaturase modifiée.
CA002326687A 1998-04-16 1999-04-16 Interconversion de desaturases et d'hydroxylases d'acides gras vegetaux Abandoned CA2326687A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US8193698P 1998-04-16 1998-04-16
US60/081,936 1998-04-16
US12316899P 1999-03-05 1999-03-05
US60/123,168 1999-03-05
PCT/US1999/008400 WO1999053073A2 (fr) 1998-04-16 1999-04-16 Interconversion de desaturases et d'hydroxylases d'acides gras vegetaux

Publications (1)

Publication Number Publication Date
CA2326687A1 true CA2326687A1 (fr) 1999-10-21

Family

ID=26766148

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002326687A Abandoned CA2326687A1 (fr) 1998-04-16 1999-04-16 Interconversion de desaturases et d'hydroxylases d'acides gras vegetaux

Country Status (6)

Country Link
EP (1) EP1071785A2 (fr)
AU (1) AU3862099A (fr)
BR (1) BR9910125A (fr)
CA (1) CA2326687A1 (fr)
IL (1) IL139062A0 (fr)
WO (1) WO1999053073A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6310194B1 (en) 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
CA2492544A1 (fr) * 2002-08-02 2004-02-12 Basf Plant Science Gmbh Modification d'huile de graines par expression d'une cytidyltransferase putative dans des plantes transgeniques
EP2806730A4 (fr) * 2012-01-23 2015-12-23 Linnaeus Plant Sciences Inc Modification du profil en acides gras de l'huile de cameline
BR112022015690A2 (pt) * 2020-02-07 2022-10-18 Isca Tech Métodos de produção de feromônios de insetos

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0668919T3 (da) * 1992-11-17 2003-09-15 Du Pont Gener for mikorsomale delta-12-fedtsyredesaturaser og beslægtede enzymer fra planter
JPH08506490A (ja) * 1993-02-05 1996-07-16 モンサント・カンパニー 植物中の改変リノレン酸及びリノール酸含量
US5801026A (en) * 1994-09-26 1998-09-01 Carnegie Institution Of Washington Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants
US6310194B1 (en) * 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
AU1293297A (en) * 1995-12-14 1997-07-03 Cargill Incorporated Plants having mutant sequences that confer altered fatty acid profiles
US6600029B1 (en) * 1995-12-19 2003-07-29 Regents Of The University Of Minnesota Metabolic engineering of polyhydroxyalkanoate monomer synthases
US5705391A (en) * 1996-08-14 1998-01-06 Associated Universities, Inc. Modified acyl-ACP desaturase
IL132393A0 (en) * 1997-04-15 2001-03-19 Commw Scient Ind Res Org Plant fatty acid epoxygenase genes and uses therefor

Also Published As

Publication number Publication date
IL139062A0 (en) 2001-11-25
WO1999053073A3 (fr) 2000-02-10
WO1999053073A2 (fr) 1999-10-21
AU3862099A (en) 1999-11-01
BR9910125A (pt) 2003-02-25
WO1999053073A9 (fr) 2000-07-20
EP1071785A2 (fr) 2001-01-31

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued