CA2329509C - Methodes et materiels ameliores de transformation - Google Patents

Methodes et materiels ameliores de transformation Download PDF

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Publication number
CA2329509C
CA2329509C CA002329509A CA2329509A CA2329509C CA 2329509 C CA2329509 C CA 2329509C CA 002329509 A CA002329509 A CA 002329509A CA 2329509 A CA2329509 A CA 2329509A CA 2329509 C CA2329509 C CA 2329509C
Authority
CA
Canada
Prior art keywords
rna
dna
nnnnnnnnnn nnnnnnnnnn
viral
replication
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002329509A
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English (en)
Other versions
CA2329509A1 (fr
Inventor
Lada Rasochova
Thomas L. German
Paul G. Ahlquist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of CA2329509A1 publication Critical patent/CA2329509A1/fr
Application granted granted Critical
Publication of CA2329509C publication Critical patent/CA2329509C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne de nouvelles méthodes et de nouveaux matériels destinés à la transformation d'une cellule hôte et à l'expression dans celle-ci d'ARN exogène. Spécifiquement, l'invention concerne des plates-formes de lancement d'ADN utilisées pour introduire un segment viral réplicant fixé à un polynucléotide exogène dans une cellule, de manière que le polynucléotide exogène soit exprimé dans ladite cellule et confère un trait détectable.
CA002329509A 1998-05-22 1999-05-21 Methodes et materiels ameliores de transformation Expired - Lifetime CA2329509C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8652698P 1998-05-22 1998-05-22
US60/086,526 1998-05-22
PCT/US1999/011250 WO1999061597A2 (fr) 1998-05-22 1999-05-21 Methodes et materiels ameliores de transformation

Publications (2)

Publication Number Publication Date
CA2329509A1 CA2329509A1 (fr) 1999-12-02
CA2329509C true CA2329509C (fr) 2009-10-06

Family

ID=22199163

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002329509A Expired - Lifetime CA2329509C (fr) 1998-05-22 1999-05-21 Methodes et materiels ameliores de transformation

Country Status (7)

Country Link
EP (1) EP1086237A2 (fr)
JP (1) JP3959965B2 (fr)
AR (1) AR020324A1 (fr)
AU (1) AU763096B2 (fr)
BR (1) BR9911065A (fr)
CA (1) CA2329509C (fr)
WO (1) WO1999061597A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0007231D0 (en) 2000-03-24 2000-05-17 Chiron Spa Modified rna for gene delivery
US6800748B2 (en) * 2001-01-25 2004-10-05 Large Scale Biology Corporation Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
EP1672066B1 (fr) 2003-10-01 2012-01-18 Japan Science and Technology Agency Fragment ADN, procédé de production de transformant pour la production de protéines, utilisation de celui-ci

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1288073C (fr) * 1985-03-07 1991-08-27 Paul G. Ahlquist Vecteur de transformation de l'arn
WO1990012107A1 (fr) * 1989-03-31 1990-10-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Systeme d'expression par recombinaison base sur le virus de la mosaique du tabac satellitaire
JPH04121200A (ja) * 1990-09-07 1992-04-22 Nippon Nohyaku Co Ltd 植物細胞におけるポリペプチドの製造法
DK0814154T3 (da) * 1993-09-15 2009-08-31 Novartis Vaccines & Diagnostic Rekombinante alfavirusvektorer
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants

Also Published As

Publication number Publication date
JP3959965B2 (ja) 2007-08-15
WO1999061597A3 (fr) 2000-03-30
AU4310199A (en) 1999-12-13
BR9911065A (pt) 2001-02-06
JP2002516089A (ja) 2002-06-04
WO1999061597A2 (fr) 1999-12-02
CA2329509A1 (fr) 1999-12-02
AR020324A1 (es) 2002-05-08
EP1086237A2 (fr) 2001-03-28
WO1999061597A9 (fr) 2000-02-24
AU763096B2 (en) 2003-07-10

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Effective date: 20190521