CA2333115A1 - Methode de detection de la toxicite cellulaire potentielle de composes - Google Patents

Methode de detection de la toxicite cellulaire potentielle de composes Download PDF

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Publication number
CA2333115A1
CA2333115A1 CA002333115A CA2333115A CA2333115A1 CA 2333115 A1 CA2333115 A1 CA 2333115A1 CA 002333115 A CA002333115 A CA 002333115A CA 2333115 A CA2333115 A CA 2333115A CA 2333115 A1 CA2333115 A1 CA 2333115A1
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CA
Canada
Prior art keywords
liposomes
test medium
test
dye
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002333115A
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English (en)
Inventor
Charles Mcclellan Morris
Leonas Leono Grinius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Procter and Gamble Co
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2333115A1 publication Critical patent/CA2333115A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne une procédure d'essai pour le criblage de la toxicité cellulaire potentielle d'un composé d'essai, comprenant les étapes suivantes: (a) fabrication de liposomes à partir d'un mélange de lipides, pour la simulation d'une composition de membrane cellulaire à analyser, les liposomes étant fabriqués dans un milieu aqueux ayant un pH correspondant ainsi au pH intérieur des liposomes; (b) incorporation des liposomes à un milieu d'essai aqueux ayant un pH d'au moins environ 0,5 pH unités supérieur au pH intérieur des liposomes, un gradient de pH existant entre le milieu d'essai et l'intérieur des liposomes; (c) incorporation d'un pigment fluorescent au milieu d'essai, le pigment ayant tendance à pénétrer dans les liposomes en raison du gradient de pH et étant sensible au pH de sorte que la fluorescence du pigment soit éliminée à l'intérieur des liposomes; (d) incorporation du composé d'essai au milieu d'essai; si le composé d'essai interrompt la structure des liposomes ou induit la dissipation du gradient de pH entre le milieu d'essai et l'intérieur des liposomes, la fluorescence du pigment n'est pas éliminée; (e) détermination de la fluorescence du milieu d'essai et comparaison avec celle des témoins.
CA002333115A 1998-06-04 1999-06-01 Methode de detection de la toxicite cellulaire potentielle de composes Abandoned CA2333115A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8797798P 1998-06-04 1998-06-04
US60/087,977 1998-06-04
PCT/IB1999/000998 WO1999063343A1 (fr) 1998-06-04 1999-06-01 Methode de detection de la toxicite cellulaire potentielle de composes

Publications (1)

Publication Number Publication Date
CA2333115A1 true CA2333115A1 (fr) 1999-12-09

Family

ID=22208381

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002333115A Abandoned CA2333115A1 (fr) 1998-06-04 1999-06-01 Methode de detection de la toxicite cellulaire potentielle de composes

Country Status (7)

Country Link
EP (1) EP1084405A1 (fr)
JP (1) JP2002517726A (fr)
AU (1) AU3842699A (fr)
CA (1) CA2333115A1 (fr)
IL (1) IL139746A0 (fr)
NO (1) NO20006125L (fr)
WO (1) WO1999063343A1 (fr)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2642526B1 (fr) * 1989-01-27 1992-11-13 Spiral Rech & Dev Utilisation de generateur de radicaux libres dans le domaine des dosages biologiques

Also Published As

Publication number Publication date
NO20006125L (no) 2001-02-02
WO1999063343A1 (fr) 1999-12-09
NO20006125D0 (no) 2000-12-01
IL139746A0 (en) 2002-02-10
EP1084405A1 (fr) 2001-03-21
JP2002517726A (ja) 2002-06-18
AU3842699A (en) 1999-12-20

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued