CA2342108A1 - Procede et dispositif de concentration de groupes selectionnes de micro-organismes - Google Patents

Procede et dispositif de concentration de groupes selectionnes de micro-organismes Download PDF

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Publication number
CA2342108A1
CA2342108A1 CA002342108A CA2342108A CA2342108A1 CA 2342108 A1 CA2342108 A1 CA 2342108A1 CA 002342108 A CA002342108 A CA 002342108A CA 2342108 A CA2342108 A CA 2342108A CA 2342108 A1 CA2342108 A1 CA 2342108A1
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CA
Canada
Prior art keywords
beads
microorganisms
enclosure
suspension
size
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002342108A
Other languages
English (en)
Inventor
Ruth F. Eden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centrus International Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2342108A1 publication Critical patent/CA2342108A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un procédé et un dispositif permettant de concentrer des organismes cibles à partir d'un mélange d'organismes. Des perles (1) fabriquées dans une matière telle que le nylon, le polystyrène ou le verre sont recouvertes d'anticorps de micro-organismes spécifiques. Ces perles (1) sont contenues dans une enceinte (2) entourée d'un matériau grillagée. La taille des pores de la grille est inférieure à la taille des perles afin de s'assurer que les perles restent à l'intérieur du matériau grillagé, et plus grande que la taille des micro-organismes pour permettre l'interaction des micro-organismes avec les perles. Une ficelle (5) est attachée à la partie supérieure de l'enceinte (2) pour permettre d'agiter le dispositif dans le milieu de croissance contenant les organismes cibles.
CA002342108A 1998-08-24 1999-08-23 Procede et dispositif de concentration de groupes selectionnes de micro-organismes Abandoned CA2342108A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9762798P 1998-08-24 1998-08-24
US60/097,627 1998-08-24
PCT/US1999/018618 WO2000010702A1 (fr) 1998-08-24 1999-08-23 Procede et dispositif de concentration de groupes selectionnes de micro-organismes

Publications (1)

Publication Number Publication Date
CA2342108A1 true CA2342108A1 (fr) 2000-03-02

Family

ID=22264348

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002342108A Abandoned CA2342108A1 (fr) 1998-08-24 1999-08-23 Procede et dispositif de concentration de groupes selectionnes de micro-organismes

Country Status (6)

Country Link
EP (1) EP1117476A4 (fr)
CN (1) CN1320060A (fr)
AU (1) AU773645B2 (fr)
CA (1) CA2342108A1 (fr)
MX (1) MXPA01001873A (fr)
WO (1) WO2000010702A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060105349A1 (en) * 2004-11-12 2006-05-18 Promega Corporation Device and method for purification of biological materials
CN101215598B (zh) * 2008-01-11 2010-11-17 中华人民共和国吉林出入境检验检疫局 免疫富集丝检测细菌的方法及免疫富集刷
CN102449460B (zh) * 2009-04-03 2014-11-12 3M创新有限公司 微生物浓集方法和装置
CN110093261B (zh) * 2019-04-28 2022-11-25 陕西科技大学 一种用于沉积物中厌氧菌原位分离的装置及使用方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3840345A (en) * 1972-06-23 1974-10-08 Co Ind De Procedes & D Applic Self agglomerating fluidized bed reacting apparatus
US3970518A (en) * 1975-07-01 1976-07-20 General Electric Company Magnetic separation of biological particles
US4230685A (en) * 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
DE3617672C2 (de) * 1986-05-26 2000-02-17 Claus Heuck Verfahren zur Herstellung eines Reagenz, ein danach hergestelltes Reagenz sowie dessen Verwendung zur Bindung von Polymeren und Mikroorganismen aus wäßrigen Lösungen
JPS635263A (ja) * 1986-06-24 1988-01-11 Yasunobu Tsukioka 血液等の検査方法
NO162946C (no) * 1987-08-21 1990-03-14 Otto Soerensen Anordning for magnetisk separasjon av celler.
FR2624401A1 (fr) * 1987-12-11 1989-06-16 Charbonnages De France Grille de fluidisation refroidie
US4931401A (en) * 1988-09-01 1990-06-05 La Societe De Recherche Snc Inc. Bioreactor
US5175093A (en) * 1989-11-07 1992-12-29 Lehigh University Bioactive cells immobilized in alginate beads containing voids formed with polyethylene glycol
US5186824A (en) * 1991-09-04 1993-02-16 Large Scale Biology Corporation System for solid phase reactions
US5342790A (en) * 1992-10-30 1994-08-30 Becton Dickinson And Company Apparatus for indirect fluorescent assay of blood samples

Also Published As

Publication number Publication date
CN1320060A (zh) 2001-10-31
AU5488699A (en) 2000-03-14
WO2000010702A1 (fr) 2000-03-02
MXPA01001873A (es) 2002-09-02
EP1117476A1 (fr) 2001-07-25
EP1117476A4 (fr) 2007-08-22
AU773645B2 (en) 2004-05-27

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