CA2352476A1 - Arrangement et procede d'analyse de sequences d'acides nucleiques - Google Patents
Arrangement et procede d'analyse de sequences d'acides nucleiques Download PDFInfo
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- CA2352476A1 CA2352476A1 CA002352476A CA2352476A CA2352476A1 CA 2352476 A1 CA2352476 A1 CA 2352476A1 CA 002352476 A CA002352476 A CA 002352476A CA 2352476 A CA2352476 A CA 2352476A CA 2352476 A1 CA2352476 A1 CA 2352476A1
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- nucleic acid
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- acid sequence
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- 229960002317 succinimide Drugs 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
- 239000007160 ty medium Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
L'invention concerne un arrangement servant à analyser une séquence d'acides nucléiques ou un mélange de séquences d'acides nucléiques. Cet arrangement comprend : a) un support, et b) au moins deux séquences d'acides nucléiques différentes, liées audit support, chaque séquence liée comprenant au moins une séquence d'acides nucléiques correspondant à la séquence d'un fragment de restriction que l'on peut obtenir par restriction d'un ADN génomique et/ou d'au moins un ADNc avec au moins une enzyme de restriction à points de coupure fréquents et au moins une enzyme de restriction à point de coupure rare. Cet arrangement comprend, de préférence, au moins 10, préférablement au moins 100, et idéalement au moins 1000 séquences d'acides nucléiques différentes liées au support. En outre, cet arrangement comprend plusieurs marqueurs AFLP~ pris à partir d'un individu ou d'un groupe d'individus apparentés. L'invention concerne également un procédé de préparation de séquences d'acides nucléiques, et notamment de marqueurs AFLP~, utiles dans un tel arrangement, de même que des procédés d'analyse d'une séquence d'acides nucléiques ou d'un mélange de séquences d'acides nucléiques au moyen d'un tel arrangement. Enfin, l'invention concerne une trousse d'éléments comprenant notamment l'arrangement de l'invention, de même que des données obtenues au moyen de cet arrangement.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98204114 | 1998-12-04 | ||
| EP98204114.7 | 1998-12-04 | ||
| PCT/NL1999/000743 WO2000034518A1 (fr) | 1998-12-04 | 1999-12-03 | Arrangement et procede d'analyse de sequences d'acides nucleiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2352476A1 true CA2352476A1 (fr) | 2000-06-15 |
Family
ID=8234431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002352476A Abandoned CA2352476A1 (fr) | 1998-12-04 | 1999-12-03 | Arrangement et procede d'analyse de sequences d'acides nucleiques |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1135524A1 (fr) |
| JP (1) | JP2002532070A (fr) |
| AU (1) | AU1696800A (fr) |
| CA (1) | CA2352476A1 (fr) |
| IL (1) | IL143486A0 (fr) |
| WO (1) | WO2000034518A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000061801A2 (fr) * | 1999-04-09 | 2000-10-19 | Keygene N.V. | METHODE DE DETECTION ET/OU D'ANALYSE, AU MOYEN DE TECHNIQUES D'EXTENSION D'AMORCES, DE POLYMORPHISMES NUCLEOTIDIQUES UNIQUES DANS DES FRAGMENTS DE RESTRICTION, ET NOTAMMENT DANS DES FRAGMENTS DE RESTRICTION AMPLIFIES GENERES PAR AFLP$m(3) |
| ATE317024T1 (de) * | 1999-12-29 | 2006-02-15 | Keygene Nv | Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden |
| MXPA02009431A (es) * | 2000-03-29 | 2005-08-26 | Cambia | Metodos para la genotipificacion mediante analisis de hibridacion. |
| US6713258B2 (en) * | 2000-03-29 | 2004-03-30 | Center For The Application Of Molecular Biology To International Agriculture (Cambia) | Methods for genotyping by hybridization analysis |
| US7141371B2 (en) | 2002-09-06 | 2006-11-28 | State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Methods for detecting and localizing DNA mutations by microarray |
| DK1885882T3 (da) * | 2005-05-10 | 2011-04-11 | State Of Oregon Acting By & Through The State Board Of Higher Eduction On Behalf Of The University O | Fremgangsmåder til kortlægning af polymorfier og polymorfi-mikroarray |
| JP5799484B2 (ja) * | 2009-12-14 | 2015-10-28 | トヨタ自動車株式会社 | Dnaマイクロアレイにおけるプローブ設計方法、当該方法により設計されたプローブを有するdnaマイクロアレイ |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0804618B1 (fr) * | 1994-11-28 | 1999-01-27 | E.I. Du Pont De Nemours And Company | Sondes de microsatellites composes pour la detection de polymorphismes genetiques |
| JP2002515738A (ja) * | 1996-01-23 | 2002-05-28 | アフィメトリックス,インコーポレイティド | 核酸分析法 |
| EP1007730A4 (fr) * | 1996-08-30 | 2004-04-28 | Invitrogen Corp | PROCEDES D'IDENTIFICATION ET D'ISOLEMENT DE SEQUENCES DE NUCLEOTIDES SPECIFIQUES DANS L'ADNc ET L'ADN GENOMIQUE |
| JP2001521754A (ja) * | 1997-10-30 | 2001-11-13 | コールド スプリング ハーバー ラボラトリー | Dna識別のためのプローブアレイ及びプローブアレイの使用方法 |
-
1999
- 1999-12-03 WO PCT/NL1999/000743 patent/WO2000034518A1/fr not_active Ceased
- 1999-12-03 EP EP99960021A patent/EP1135524A1/fr not_active Withdrawn
- 1999-12-03 CA CA002352476A patent/CA2352476A1/fr not_active Abandoned
- 1999-12-03 AU AU16968/00A patent/AU1696800A/en not_active Abandoned
- 1999-12-03 JP JP2000586951A patent/JP2002532070A/ja active Pending
- 1999-12-03 IL IL14348699A patent/IL143486A0/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1135524A1 (fr) | 2001-09-26 |
| JP2002532070A (ja) | 2002-10-02 |
| AU1696800A (en) | 2000-06-26 |
| IL143486A0 (en) | 2002-04-21 |
| WO2000034518A1 (fr) | 2000-06-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued | ||
| FZDE | Discontinued |
Effective date: 20041203 |