CA2353578A1 - Application de contrainte de cisaillement par ecoulement a des cellules de muscle lisse pour la production d'implants - Google Patents

Application de contrainte de cisaillement par ecoulement a des cellules de muscle lisse pour la production d'implants Download PDF

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Publication number
CA2353578A1
CA2353578A1 CA002353578A CA2353578A CA2353578A1 CA 2353578 A1 CA2353578 A1 CA 2353578A1 CA 002353578 A CA002353578 A CA 002353578A CA 2353578 A CA2353578 A CA 2353578A CA 2353578 A1 CA2353578 A1 CA 2353578A1
Authority
CA
Canada
Prior art keywords
cells
shear flow
smooth muscle
scaffold
flow stress
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002353578A
Other languages
English (en)
Inventor
Ann A. Lee
Joan Zeltinger
Stephanie M. Kladakis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advanced Tissue Sciences Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2353578A1 publication Critical patent/CA2353578A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Vascular Medicine (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention se rapporte à des procédés de culture de cellules de muscle lisse destinées à la production de greffons tissulaires ou d'autres structures de remplacement implantables. L'invention concerne plus spécifiquement l'application de contrainte de cisaillement par écoulement appliquée à des cellules de muscle lisse en culture, dans laquelle les cellules prennent un alignement perpendiculaire à la direction de l'écoulement, alignement qui est proche de l'orientation des cellules in vivo. Les cultures résultantes et les procédés sont utiles pour la production de greffons vasculaires améliorés, de vaisseaux et d'autres structures implantables destinées à la correction de défauts ou de tissus anormaux du corps.
CA002353578A 1998-12-11 1999-12-10 Application de contrainte de cisaillement par ecoulement a des cellules de muscle lisse pour la production d'implants Abandoned CA2353578A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11176098P 1998-12-11 1998-12-11
US60/111,760 1998-12-11
PCT/US1999/029257 WO2000034442A2 (fr) 1998-12-11 1999-12-10 Application de contrainte de cisaillement par ecoulement a des cellules de muscle lisse pour la production d'implants

Publications (1)

Publication Number Publication Date
CA2353578A1 true CA2353578A1 (fr) 2000-06-15

Family

ID=22340317

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002353578A Abandoned CA2353578A1 (fr) 1998-12-11 1999-12-10 Application de contrainte de cisaillement par ecoulement a des cellules de muscle lisse pour la production d'implants

Country Status (6)

Country Link
EP (1) EP1144596A2 (fr)
JP (1) JP2002531118A (fr)
AU (1) AU2171900A (fr)
CA (1) CA2353578A1 (fr)
IL (1) IL143564A0 (fr)
WO (1) WO2000034442A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1782849A3 (fr) * 1999-04-12 2007-05-23 Theregen, Inc. Tissu stromal tridimensionnel
US20030007954A1 (en) * 1999-04-12 2003-01-09 Gail K. Naughton Methods for using a three-dimensional stromal tissue to promote angiogenesis
DE10061704A1 (de) * 2000-12-12 2002-06-20 Hans Joerg Bauer Verfahren und Vorrichtung zur Herstellung von biologischem Gewebe in einer Wachstumskammer
JP2002315566A (ja) * 2001-04-24 2002-10-29 Takagi Ind Co Ltd 細胞・組織培養装置
EP1458428A1 (fr) * 2001-11-28 2004-09-22 Universit Laval Procede permettant d'ameliorer la fonctionnalite de constructions tissulaires
US20050019488A1 (en) * 2001-11-30 2005-01-27 Cambridge Polymer Group, Inc., Boston, Ma Layered aligned polymer structures and methods of making same
US7048963B2 (en) 2001-11-30 2006-05-23 Cambridge Polymers Group, Inc. Layered aligned polymer structures and methods of making same
US7387645B2 (en) * 2003-04-25 2008-06-17 Medtronic Vascular, Inc. Cellular therapy to heal vascular tissue
US7384786B2 (en) 2003-07-16 2008-06-10 Scimed Life Systems, Inc. Aligned scaffolds for improved myocardial regeneration
US7738682B2 (en) * 2003-10-22 2010-06-15 Medtrain Technologies, Llc Tissue engineered construct analytical imaging system and method of obtaining and analyzing images of tissue engineered constructs
US9493735B2 (en) 2005-04-15 2016-11-15 Wake Forest University Health Sciences Bioreactor system and method of enhancing functionality of muscle cultured in vitro
WO2007074500A1 (fr) * 2005-12-26 2007-07-05 Osaka University Procédé de culture de tissu en trois dimensions, ayant une configuration dans l'organisme vivant
JP4989108B2 (ja) 2006-05-25 2012-08-01 Ｊｎｃ株式会社 血管平滑筋細胞の培養方法、培養器材および培養によって得られる医療用材料
US8058057B2 (en) 2008-05-30 2011-11-15 Corning Incorporated Cell culture apparatus and method
AU2010233169A1 (en) 2009-04-09 2011-11-03 Arizona Board Of Regents On Behalf Of The University Of Arizona Cellular seeding and co-culture of a three dimensional fibroblast construct
US8507263B2 (en) 2009-08-07 2013-08-13 Maria Adelaide Asnaghi Rotating bioreactor
WO2012021814A2 (fr) 2010-08-13 2012-02-16 Wake Forest University Health Sciences Méthodes de préparation in vitro d'un substitut de réparation musculaire destiné à être implanté in vivo
WO2014174899A1 (fr) * 2013-04-23 2014-10-30 国立大学法人徳島大学 Cellule compressée ou tissu compressé en tant que modèle cellulaire d'affection pathologique et procédé de fabrication associé
CA3067558A1 (fr) 2017-06-16 2018-12-20 Avery Therapeutics, Inc. Compositions de tissu tridimensionnel et procedes d'utilisation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2195095A (en) * 1994-03-24 1995-10-09 University Of Washington Devices and methods for implanting transduced cells
US5792603A (en) * 1995-04-27 1998-08-11 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Also Published As

Publication number Publication date
IL143564A0 (en) 2002-04-21
WO2000034442A2 (fr) 2000-06-15
JP2002531118A (ja) 2002-09-24
EP1144596A2 (fr) 2001-10-17
WO2000034442A3 (fr) 2001-11-01
AU2171900A (en) 2000-06-26

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20031210