CA2366488C - Optimisation de l'affinite de ligands four des cibles d'arn faisant appel a la spectrometrie de masse - Google Patents

Optimisation de l'affinite de ligands four des cibles d'arn faisant appel a la spectrometrie de masse Download PDF

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Publication number
CA2366488C
CA2366488C CA2366488A CA2366488A CA2366488C CA 2366488 C CA2366488 C CA 2366488C CA 2366488 A CA2366488 A CA 2366488A CA 2366488 A CA2366488 A CA 2366488A CA 2366488 C CA2366488 C CA 2366488C
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Canada
Prior art keywords
ligand
target
rna
ligands
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2366488A
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English (en)
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CA2366488A1 (fr
Inventor
Richard Griffey
Steven Hofstadler
Jared J. Drader
Kristin S. Lowrey
Venkatraman Mohan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ionis Pharmaceuticals Inc
Original Assignee
Isis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/499,875 external-priority patent/US6770486B1/en
Priority claimed from US09/573,479 external-priority patent/US8188011B1/en
Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc
Publication of CA2366488A1 publication Critical patent/CA2366488A1/fr
Application granted granted Critical
Publication of CA2366488C publication Critical patent/CA2366488C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés d'identification de composés de ligands se liant à des molécules cibles, notamment des protéines ou des ARN structurés avec une affinité de l'ordre du millimole (mM) faisant appel à la spectrométrie de masse. Ces procédés sont utilisés pour déterminer le mode d'interaction de liaison entre deux ou plusieurs composés de ligands avec la cible ainsi que leurs affinités relatives. Font aussi l'objet de cette invention des procédés de conception de composés présentant une affinité plus importante avec une molécule cible par identification de deux ligands au moins grâce à la spectrométrie de masse et de liaison des ligands entre eux afin d'obtenir un nouveau composé.
CA2366488A 2000-02-08 2001-02-08 Optimisation de l'affinite de ligands four des cibles d'arn faisant appel a la spectrometrie de masse Expired - Lifetime CA2366488C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/499,875 2000-02-08
US09/499,875 US6770486B1 (en) 2000-02-08 2000-02-08 Optimization of ligand affinity for RNA targets using mass spectrometry
US09/573,479 2000-05-16
US09/573,479 US8188011B1 (en) 2000-02-08 2000-05-16 Optimization of ligand affinity for RNA targets using mass spectrometry
PCT/US2001/040064 WO2001058573A1 (fr) 2000-02-08 2001-02-08 Optimisation de l'affinite de ligands four des cibles d'arn faisant appel a la spectrometrie de masse

Publications (2)

Publication Number Publication Date
CA2366488A1 CA2366488A1 (fr) 2001-08-16
CA2366488C true CA2366488C (fr) 2012-07-03

Family

ID=27053323

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2366488A Expired - Lifetime CA2366488C (fr) 2000-02-08 2001-02-08 Optimisation de l'affinite de ligands four des cibles d'arn faisant appel a la spectrometrie de masse

Country Status (4)

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EP (1) EP1183091A4 (fr)
JP (1) JP2003522331A (fr)
AU (1) AU763209B2 (fr)
CA (1) CA2366488C (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202004961D0 (en) 2020-04-03 2020-05-20 Micromass Ltd De-clustering guide
EP4089717A1 (fr) * 2021-05-10 2022-11-16 Tofwerk AG Procédé d'ionisation chimique et réacteur ion-molécule
GB2624012B (en) * 2022-11-04 2026-03-25 Thermo Fisher Scient Bremen Gmbh Enhancing mass spectrometer signals

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6428956B1 (en) * 1998-03-02 2002-08-06 Isis Pharmaceuticals, Inc. Mass spectrometric methods for biomolecular screening

Also Published As

Publication number Publication date
AU763209B2 (en) 2003-07-17
JP2003522331A (ja) 2003-07-22
AU4796401A (en) 2001-08-20
CA2366488A1 (fr) 2001-08-16
EP1183091A1 (fr) 2002-03-06
EP1183091A4 (fr) 2005-03-02

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