CA2367488A1 - Vecteurs retroviraux comportant des sites donneurs et accepteurs d'epissure, fonctionnels et non fonctionnels - Google Patents

Vecteurs retroviraux comportant des sites donneurs et accepteurs d'epissure, fonctionnels et non fonctionnels Download PDF

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Publication number
CA2367488A1
CA2367488A1 CA002367488A CA2367488A CA2367488A1 CA 2367488 A1 CA2367488 A1 CA 2367488A1 CA 002367488 A CA002367488 A CA 002367488A CA 2367488 A CA2367488 A CA 2367488A CA 2367488 A1 CA2367488 A1 CA 2367488A1
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Prior art keywords
vector
retroviral
viral
site
cell
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CA002367488A
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Mark Uden
Kyriacos Mitrophanous
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Oxford Biomedica UK Ltd
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Abstract

Ce vecteur rétroviral comporte un site fonctionnel donneur d'épissure (FSDS) et un site fonctionnel accepteur d'épissure (FSAS). Le FSDS et le FSAS flanquent une première séquence nucléotidique, le FSDS se trouvant en amont du FSAS. Ce vecteur rétroviral est dérivé d'un pro-vecteur rétroviral, lequel comporte une première séquence nucléotidique capable de fournir le site fonctionnel donneur d'épissure ainsi qu'une deuxième séquence nucléotidique capable de fournir le site fonctionnel accepteur d'épissure. Ce pro-vecteur comporte également une troisième séquence nucléotidique, capable de fournir un site non fonctionnel donneur d'épissure (NFSDS), ainsi qu'une quatrième, capable de fournir un site non fonctionnel d'épissure (NFSS). La première séquence nucléotidique se trouve en aval de la deuxième, la troisième et la quatrième étant en amont de la deuxième, de sorte, qu'après transcription inverse du pro-vecteur rétroviral sur un site cible désiré, le vecteur rétroviral est à même d'être épissé.
CA002367488A 1999-03-22 2000-03-22 Vecteurs retroviraux comportant des sites donneurs et accepteurs d'epissure, fonctionnels et non fonctionnels Abandoned CA2367488A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9906615.1 1999-03-22
GBGB9906615.1A GB9906615D0 (en) 1999-03-22 1999-03-22 Vector
PCT/GB2000/001091 WO2000056910A1 (fr) 1999-03-22 2000-03-22 Vecteurs retroviraux comportant des sites donneurs et accepteurs d'epissure, fonctionnels et non fonctionnels

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Publication Number Publication Date
CA2367488A1 true CA2367488A1 (fr) 2000-09-28

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CA002367488A Abandoned CA2367488A1 (fr) 1999-03-22 2000-03-22 Vecteurs retroviraux comportant des sites donneurs et accepteurs d'epissure, fonctionnels et non fonctionnels

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EP (1) EP1163356A1 (fr)
JP (1) JP2002539797A (fr)
KR (1) KR20020003215A (fr)
CN (1) CN1344326A (fr)
AU (1) AU3312800A (fr)
CA (1) CA2367488A1 (fr)
GB (2) GB9906615D0 (fr)
WO (1) WO2000056910A1 (fr)
ZA (1) ZA200106031B (fr)

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Publication number Priority date Publication date Assignee Title
US7052904B2 (en) 2000-01-31 2006-05-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Hybrid adeno-retroviral vector for the transfection of cells
US7226779B2 (en) 2001-01-30 2007-06-05 The United States Of America As Represented By The Department Of Health And Human Services Hybrid adenoviral vector
CN109679994B (zh) * 2018-12-13 2021-02-02 湖北汇智铭传生物科技股份有限公司 通过四环素诱导表达外源基因的游离载体及其构建方法
SG11202106356QA (en) * 2018-12-20 2021-07-29 Vigeneron Gmbh An optimized acceptor splice site module for biological and biotechnological applications
GB202005096D0 (en) * 2020-04-07 2020-05-20 Glaxosmithkline Modified vectors for production of retrovirus
CN113549656A (zh) * 2021-09-23 2021-10-26 山东维真生物科技有限公司 一种用于多基因转化的慢病毒载体表达系统

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Publication number Priority date Publication date Assignee Title
US5631162A (en) * 1993-06-11 1997-05-20 Massachusetts Institute Of Technology Retroviral vectors for transducing β-globin gene and β-locus control region derivatives
WO1996028563A1 (fr) * 1995-03-09 1996-09-19 Bavarian Nordic Vecteurs qui portent des genes therapeutiques codant des peptides antimicrobiens pour la therapie genique
GB9720465D0 (en) * 1997-09-25 1997-11-26 Oxford Biomedica Ltd Dual-virus vectors
KR20010052076A (ko) * 1997-09-23 2001-06-25 찰스 굿맨 허혈 조건에서 헤마토포이에틱 스템 세포의 유전자 발현

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WO2000056910A1 (fr) 2000-09-28
EP1163356A1 (fr) 2001-12-19
KR20020003215A (ko) 2002-01-10
GB2362886A (en) 2001-12-05
AU3312800A (en) 2000-10-09
JP2002539797A (ja) 2002-11-26
ZA200106031B (en) 2002-09-18
CN1344326A (zh) 2002-04-10
GB9906615D0 (en) 1999-05-19
GB0120709D0 (en) 2001-10-17

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