CA2369524A1 - Extension nucleotidique sur un micro-arrangement d'amorces immobilisees au moyen d'un gel - Google Patents
Extension nucleotidique sur un micro-arrangement d'amorces immobilisees au moyen d'un gel Download PDFInfo
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- CA2369524A1 CA2369524A1 CA002369524A CA2369524A CA2369524A1 CA 2369524 A1 CA2369524 A1 CA 2369524A1 CA 002369524 A CA002369524 A CA 002369524A CA 2369524 A CA2369524 A CA 2369524A CA 2369524 A1 CA2369524 A1 CA 2369524A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne des méthodes et des compositions destinées à l'extension nucléotidique d'amorces immobilisées dans des matrices de gel sur une microplaquette utilisant des amorces définissant plusieurs bases ou des séries multiples d'amorces, ou des combinaisons de celles-ci. Des molécules ou des fractions de molécules sont identifiées. L'extension à base unique a été amplifiée par l'exécution de la réaction dans des conditions de hautes températures.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30067599A | 1999-04-27 | 1999-04-27 | |
| US09/300,675 | 1999-04-27 | ||
| PCT/US2000/011286 WO2000065098A2 (fr) | 1999-04-27 | 2000-04-25 | Extension nucleotidique sur un micro-arrangement d'amorces immobilisees au moyen d'un gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2369524A1 true CA2369524A1 (fr) | 2000-11-02 |
Family
ID=23160129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002369524A Abandoned CA2369524A1 (fr) | 1999-04-27 | 2000-04-25 | Extension nucleotidique sur un micro-arrangement d'amorces immobilisees au moyen d'un gel |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1171637A2 (fr) |
| AU (1) | AU4669100A (fr) |
| CA (1) | CA2369524A1 (fr) |
| WO (1) | WO2000065098A2 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7179638B2 (en) | 1999-07-30 | 2007-02-20 | Large Scale Biology Corporation | Microarrays and their manufacture by slicing |
| US6713309B1 (en) | 1999-07-30 | 2004-03-30 | Large Scale Proteomics Corporation | Microarrays and their manufacture |
| WO2001085988A1 (fr) * | 2000-05-09 | 2001-11-15 | Diatech Pty. Ltd. | Methodes de detection de molecules d'acide nucleique comportant des sequences nucleotidiques particulieres |
| EP1373557A4 (fr) * | 2000-12-28 | 2004-07-14 | Engelhardt Inst Of Molecular B | Utilisation de micropuces oligonucleotidiques generiques dans la detection d'interactions proteines et acides nucleiques |
| WO2003033691A1 (fr) * | 2001-10-19 | 2003-04-24 | Institut Molekulyarnoi Biologii Im. V.A. Engelgardta Rossiiskoi Akademii Nauk | Micropuce cellulaire et son utilisation dans un procede d'analyse de cellules vivantes |
| US7049073B2 (en) | 2002-10-30 | 2006-05-23 | The University Of Chicago | Double stranded nucleic acid biochips |
| JP6269059B2 (ja) * | 2012-03-29 | 2018-01-31 | 三菱ケミカル株式会社 | βグロビン遺伝子の変異を検出するためのマイクロアレイ及びその検出方法 |
| CN110678557A (zh) | 2017-03-31 | 2020-01-10 | 玛赛拉·基亚里 | 通过管内杂交与通用标签-微阵列的组合的突变的基因分型 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2044591C (fr) * | 1989-02-13 | 2002-08-13 | James Langham Dale | Determination des sequences nucleotidiques ou de modifications de celles-ci |
| US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| RU1794088C (ru) * | 1991-03-18 | 1993-02-07 | Институт Молекулярной Биологии Ан@ Ссср | Способ определени нуклеотидной последовательности ДНК и устройство дл его осуществлени |
| EP0607151B1 (fr) * | 1992-06-17 | 2002-11-13 | City Of Hope | Procede de detection de sequences d'acide nucleique et de discrimination entre ces sequences |
| ATE244406T1 (de) * | 1993-05-10 | 2003-07-15 | Nissui Pharm Co Ltd | Verfahren zur bestimmung von mehr als einem immunologischen liganden und bestimmungsreagenz sowie satz dafuer |
| RU2041261C1 (ru) * | 1993-08-11 | 1995-08-09 | Институт молекулярной биологии им.В.А.Энгельгардта РАН | Способ изготовления матрицы для детектирования мисматчей |
| GB9507238D0 (en) * | 1995-04-07 | 1995-05-31 | Isis Innovation | Detecting dna sequence variations |
| AUPO427996A0 (en) * | 1996-12-20 | 1997-01-23 | Co-Operative Research Centre For Diagnostic Technologies | Method for detecting a nucleotide at a specific location within a polynucleotide sequence and apparatus therefor |
| JPH10253632A (ja) * | 1997-03-10 | 1998-09-25 | Nissui Pharm Co Ltd | 分析方法、キット及び装置 |
| US20020055100A1 (en) * | 1997-04-01 | 2002-05-09 | Kawashima Eric H. | Method of nucleic acid sequencing |
-
2000
- 2000-04-25 WO PCT/US2000/011286 patent/WO2000065098A2/fr not_active Ceased
- 2000-04-25 CA CA002369524A patent/CA2369524A1/fr not_active Abandoned
- 2000-04-25 EP EP00928451A patent/EP1171637A2/fr not_active Withdrawn
- 2000-04-25 AU AU46691/00A patent/AU4669100A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2000065098A2 (fr) | 2000-11-02 |
| WO2000065098A3 (fr) | 2001-07-19 |
| AU4669100A (en) | 2000-11-10 |
| EP1171637A2 (fr) | 2002-01-16 |
| WO2000065098A9 (fr) | 2001-08-09 |
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