CA2391571A1 - Procede d'identification rapide et precise de micro-organismes - Google Patents

Procede d'identification rapide et precise de micro-organismes Download PDF

Info

Publication number
CA2391571A1
CA2391571A1 CA002391571A CA2391571A CA2391571A1 CA 2391571 A1 CA2391571 A1 CA 2391571A1 CA 002391571 A CA002391571 A CA 002391571A CA 2391571 A CA2391571 A CA 2391571A CA 2391571 A1 CA2391571 A1 CA 2391571A1
Authority
CA
Canada
Prior art keywords
primer
target sequence
sequence
oligonucleotide
organism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002391571A
Other languages
English (en)
Inventor
Zhiping Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Apollo Biotechnology Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2391571A1 publication Critical patent/CA2391571A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et des compositions utilisés pour l'identification rapide de micro-organismes. L'invention concerne également des procédés et des compositions servant à l'identification rapide et simultanée dans un échantillon biologique de multiples micro-organismes, comportant des bactéries, des champignons, des virus et de la levure. L'invention fait également état de procédés et de compositions employant de techniques d'amplification et d'hybridation de séquence spécifique, afin de détecter d'une séquence de polynucléotide spécifique d'une espèce dans un échantillon. L'invention présente aussi de nouveaux procédés associant des sondes d'oligonucléotides à des surfaces en verre à l'efficacité améliorée.
CA002391571A 1999-11-16 2000-11-16 Procede d'identification rapide et precise de micro-organismes Abandoned CA2391571A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16588199P 1999-11-16 1999-11-16
US60/165,881 1999-11-16
US47945700A 2000-01-06 2000-01-06
US09/479,457 2000-01-06
PCT/US2000/031579 WO2001036683A2 (fr) 1999-11-16 2000-11-16 Procede d'identification rapide et precise de micro-organismes

Publications (1)

Publication Number Publication Date
CA2391571A1 true CA2391571A1 (fr) 2001-05-25

Family

ID=26861776

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002391571A Abandoned CA2391571A1 (fr) 1999-11-16 2000-11-16 Procede d'identification rapide et precise de micro-organismes

Country Status (4)

Country Link
EP (1) EP1261716A2 (fr)
AU (1) AU1771901A (fr)
CA (1) CA2391571A1 (fr)
WO (1) WO2001036683A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1421216A4 (fr) * 2001-09-01 2006-05-17 Samsung Electronics Co Ltd Procede pour fabriquer une biopuce en hydrogel au moyen d'un derive de polyethyleneglycol etoile comportant un groupe epoxy
JP2003144153A (ja) * 2001-11-09 2003-05-20 Gifu Univ 遺伝子検出方法、遺伝子検出用プライマー、ｄｎａマイクロアレイ及び遺伝子検出用キット
US20050202414A1 (en) * 2001-11-15 2005-09-15 The Regents Of The University Of California Apparatus and methods for detecting a microbe in a sample
US6881835B2 (en) * 2002-01-04 2005-04-19 Dr. Chip Biotechnology Inc. Detection of respiratory viruses
DE10244456A1 (de) * 2002-09-24 2004-04-15 Hain Lifescience Gmbh Verfahren zum Nachweis und Differenzierung von Bakterien
FI113549B (fi) * 2002-11-19 2004-05-14 Mobidiag Oy Diagnostinen menetelmä hengitystieinfektioita aiheuttavien bakteerien osoittamiseksi ja tunnistamiseksi ja menetelmässä käyttökelpoinen alukeseos
FR2860801B1 (fr) * 2003-10-10 2007-09-21 Bertin Technologies Sa Methode de detection rapide de micro-organismes sur puces a adn
US20090197249A1 (en) * 2004-11-01 2009-08-06 George Mason University Compositions and methods for diagnosing colon disorders
RU2348695C2 (ru) * 2006-05-23 2009-03-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа
JP5596894B2 (ja) * 2006-11-10 2014-09-24 キヤノン株式会社 プローブセット、プローブ固定担体及び遺伝子検査方法
MX345230B (es) 2010-02-24 2017-01-20 The Broad Inst Inc * Metodos para diagnosticar patogenos de enfermedad infecciosa y su sensibilidad a farmacos.
GB201314873D0 (en) * 2013-08-20 2013-10-02 Univ Cape Town Method for diagnosing tuberculosis in a urine sample
EP3607096A1 (fr) * 2017-04-03 2020-02-12 Bio-Me AS Procédés de détection de micro-organismes
CN119040455B (zh) * 2024-10-31 2025-02-25 南京农业大学三亚研究院 高通量靶向检测反刍动物病原体及耐药基因的引物组、试剂盒及其应用
CN120738374A (zh) * 2025-08-27 2025-10-03 北京昭衍生物技术有限公司 一种用于检测螺原体的qPCR引物探针组合物、试剂盒及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4992172A (en) * 1987-09-14 1991-02-12 Gelman Sciences, Inc. Blotting methods using polyaldehyde activated membranes
US5708160A (en) * 1995-04-26 1998-01-13 The National Research Council HSP-60 genomic locus and primers for species identification
US5994066A (en) * 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
WO1998028444A2 (fr) * 1996-12-23 1998-07-02 The University Of Chicago Micropuces oligonucleotidiques sur mesure utilisees comme capteurs multiples
DE19716456C2 (de) * 1997-04-21 2002-05-08 Schmitt Heinz Josef Verwendung einer Kombination von Primern zum Nachweis von Erregern von Infektionskrankheiten
US6159949A (en) * 1997-08-29 2000-12-12 Smithkline Beecham Corporation FtsY of Staphyloccus aureus

Also Published As

Publication number Publication date
WO2001036683A2 (fr) 2001-05-25
WO2001036683A3 (fr) 2002-09-26
AU1771901A (en) 2001-05-30
EP1261716A2 (fr) 2002-12-04

Similar Documents

Publication Publication Date Title
US6656689B2 (en) Hybridization probes derived from the spacer region between the 16S and 23S rRNA genes for the detection of non-viral microorganisms
KR101038519B1 (ko) 인체 감염성질환 병원체 감별진단 및 이의 항생제 내성 유무 판별방법, 멀티플렉스 키트 그리고 이를 포함하는 칩
US20060160121A1 (en) Probe arrays for detecting multiple strains of different species
US20130157876A1 (en) Systems and Methods for Detecting Antibiotic Resistance
US20060046246A1 (en) Genus, group, species and/or strain specific 16S rDNA sequences
CA2391571A1 (fr) Procede d'identification rapide et precise de micro-organismes
US20030134293A1 (en) Method for rapid and accurate identification of microorganisms
US5536638A (en) Hybridization probes derived from the spacer region between the 16S and 23S rRNA genes for the detection of Neisseria gonorrhoeae
CN101287844A (zh) 用于检测和/或鉴定微生物的方法
US7659388B2 (en) Method and compositions for detecting respiratory disease-causing bacterial species
JPH08510920A (ja) Ｈａｅｍｏｐｈｉｌｕｓｉｎｆｌｕｅｎｚａｅを検出するための核酸プローブ
US20040253617A1 (en) Molecular typing of group B streptococci
JP2006509500A (ja) トポイソメラーゼ遺伝子の領域からの核酸プローブ及びブロードレンジプライマー並びにそれらを使用する方法
CA2428646A1 (fr) Groupe et utilisations connexes
EP1725681A2 (fr) Dosage pour la detection et l'identification de micro-organismes
KR100973435B1 (ko) 식중독균 검출용 올리고뉴클레오티드 프로브 세트 및 상기프로브 세트를 포함하는 마이크로어레이
EP1739193A2 (fr) Sérotypage de Salmonella
EP1466011B1 (fr) Nouvelles amorces pour la detection et l'identification de groupes d'indicateurs bacteriens
US20100167951A1 (en) Dna chip for detection of staphylococcus aureus
KR20090124021A (ko) 엔테로박테리아 속 식중독균 검출용 올리고뉴클레오티드프로브 세트 및 상기 프로브 세트를 포함하는마이크로어레이
KR100617647B1 (ko) 아시네토박터 바우마니 균의 검출용 ｄｎａ 칩
KR100973432B1 (ko) 바실랄레스 목 식중독균 검출용 올리고뉴클레오티드 프로브세트 및 상기 프로브 세트를 포함하는 마이크로어레이
KR20070069680A (ko) 스타필로코코스 아우레우스 검출용 ｄｎａ 칩
KR20030087912A (ko) Ｉｔｓ로부터 유도된 세균성 감염질환 원인균의 검출용ｄｎａ탐침
KR20030087913A (ko) 23Ｓ ｒＲＮＡ 유전자로부터 유도된 세균성 감염질환원인균의 검출용 ＤＮＡ 탐침

Legal Events

Date Code Title Description
FZDE Dead