CA2392010A1 - Utilisation de vecteurs lentiviraux pour la presentation de l'antigene dans les cellules dendritiques - Google Patents
Utilisation de vecteurs lentiviraux pour la presentation de l'antigene dans les cellules dendritiques Download PDFInfo
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- CA2392010A1 CA2392010A1 CA002392010A CA2392010A CA2392010A1 CA 2392010 A1 CA2392010 A1 CA 2392010A1 CA 002392010 A CA002392010 A CA 002392010A CA 2392010 A CA2392010 A CA 2392010A CA 2392010 A1 CA2392010 A1 CA 2392010A1
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- C12N2740/00—Reverse transcribing RNA viruses
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- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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Abstract
La présente invention concerne des méthodes destinées à induire une réaction immunitaire contre un antigène chez un sujet par l'administration d'une dose thérapeutiquement efficace d'une cellule dendritique, ou d'un progéniteur de ladite cellule, transduite avec un vecteur lentiviral pseudotype à réplication déficiente contenant une séquence d'acide nucléique codant un polypeptide, qui est exprimée dans les cellules dendritiques et qui est présentée à la surface de la cellule dendritique, de manière que, après avoir été administrée à un sujet, une réaction immunitaire soit induite contre l'antigène chez le sujet en question. L'invention concerne également une méthode permettant d'induire une réaction immunitaire contre un antigène chez un sujet par transduction d'un cellule dendritique, ou d'un progéniteur de ladite cellule, avec un vecteur lentiviral pseudotype contenant une séquence d'acide nucléique codant un polypeptide, qui est exprimée dans la cellule dentritique et qui est présenté à la surface de la cellule dendritique, et par la mise en contact de la cellule dendritique avec un lymphocyte T, de manière à produire un lymphocyte T activé. L'invention concerne, en outre, une méthode permettant d'activer un lymphocyte T par la mise en contact dudit lymphocyte avec une cellule dendritique, ou un progéniteur de ladite cellule, qui exprime un antigène codé par un vecteur lentiviral pseudotype transduit, au niveau de sa surface. L'invention concerne enfin un vecteur, qui contient une protéine d'enveloppe provenant d'un virus autre qu'un lentivirus, et un génome lentiviral codant des produits géniques env et nef non fonctionnels ; ou un génome lentiviral codant des produits géniques env et nef et au moins trois produits géniques de protéines accessoires, notamment les produits non fonctionnels vif, vpr et vpu.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38471999A | 1999-08-27 | 1999-08-27 | |
| US09/384,719 | 1999-08-27 | ||
| PCT/US2000/023162 WO2001016342A1 (fr) | 1999-08-27 | 2000-08-23 | Utilisation de vecteurs lentiviraux pour la presentation de l'antigene dans les cellules dendritiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2392010A1 true CA2392010A1 (fr) | 2001-03-08 |
Family
ID=23518472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002392010A Abandoned CA2392010A1 (fr) | 1999-08-27 | 2000-08-23 | Utilisation de vecteurs lentiviraux pour la presentation de l'antigene dans les cellules dendritiques |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1214437A1 (fr) |
| AU (1) | AU6930200A (fr) |
| CA (1) | CA2392010A1 (fr) |
| WO (1) | WO2001016342A1 (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2221600A (en) | 1998-12-31 | 2000-07-31 | Chiron Corporation | Improved expression of hiv polypeptides and production of virus-like particles |
| AU2487300A (en) | 1998-12-31 | 2000-07-31 | Chiron Corporation | Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof |
| EP1411770A4 (fr) | 2001-07-05 | 2006-05-10 | Chiron Corp | Polynucleotides codant des polypeptides de type c du vih antigeniques, polypeptides et leurs utilisations |
| US20030170614A1 (en) | 2001-08-31 | 2003-09-11 | Megede Jan Zur | Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof |
| CA2505403A1 (fr) * | 2002-11-07 | 2004-05-27 | Lung-Ji Chang | Cellules dendritiques modifiees |
| EP1885186B1 (fr) * | 2005-06-01 | 2015-09-02 | California Institute Of Technology | Procede permettant le transfert cible de genes au moyen de vecteurs viraux |
| US8329162B2 (en) | 2006-07-21 | 2012-12-11 | California Institute Of Technology | Targeted gene delivery for dendritic cell vaccination |
| RS53257B2 (sr) | 2009-07-24 | 2018-06-29 | Immune Design Corp | Lentiviralni vektori pseudotipizirani sa omotačem od glikobelančevine iz sindbis-virusa |
| US9713635B2 (en) | 2012-03-30 | 2017-07-25 | Immune Design Corp. | Materials and methods for producing improved lentiviral vector particles |
| WO2013149167A1 (fr) | 2012-03-30 | 2013-10-03 | Immune Design Corp. | Particules de vecteur lentiviral ayant une meilleure efficacité de transduction pour des cellules exprimant dc-sign |
| US8323662B1 (en) | 2012-03-30 | 2012-12-04 | Immune Design Corp. | Methods useful for generating highly mannosylated pseudotyped lentiviral vector particles comprising a Vpx protein |
| US9493789B2 (en) * | 2014-04-24 | 2016-11-15 | Miltenyi Biotec Gmbh | Automated generation of genetically modified stem cells |
| EP3472281A4 (fr) | 2016-06-16 | 2020-02-19 | Nuseed Pty Ltd | Événement élite canola ns-b50027-4 |
| GB202105278D0 (en) * | 2021-04-13 | 2021-05-26 | Imperial College Innovations Ltd | Cell therapy |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010007659A1 (en) * | 1997-04-17 | 2001-07-12 | The University Of California | Use of lentiviral vectors for antigen presentation in dendritic cells |
-
2000
- 2000-08-23 CA CA002392010A patent/CA2392010A1/fr not_active Abandoned
- 2000-08-23 AU AU69302/00A patent/AU6930200A/en not_active Abandoned
- 2000-08-23 EP EP00957722A patent/EP1214437A1/fr not_active Withdrawn
- 2000-08-23 WO PCT/US2000/023162 patent/WO2001016342A1/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001016342A9 (fr) | 2002-09-19 |
| AU6930200A (en) | 2001-03-26 |
| EP1214437A1 (fr) | 2002-06-19 |
| WO2001016342A1 (fr) | 2001-03-08 |
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