CA2396015A1 - Reactif d'enveloppe a viscosite elevee pour cytometrie de flux - Google Patents
Reactif d'enveloppe a viscosite elevee pour cytometrie de flux Download PDFInfo
- Publication number
- CA2396015A1 CA2396015A1 CA002396015A CA2396015A CA2396015A1 CA 2396015 A1 CA2396015 A1 CA 2396015A1 CA 002396015 A CA002396015 A CA 002396015A CA 2396015 A CA2396015 A CA 2396015A CA 2396015 A1 CA2396015 A1 CA 2396015A1
- Authority
- CA
- Canada
- Prior art keywords
- fluid
- viscosity
- flow
- increasing agent
- polyvinyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 239000003153 chemical reaction reagent Substances 0.000 title description 4
- 239000012530 fluid Substances 0.000 claims abstract description 106
- 239000002245 particle Substances 0.000 claims abstract description 48
- 238000004458 analytical method Methods 0.000 claims abstract description 41
- 229920002554 vinyl polymer Polymers 0.000 claims abstract description 18
- HNJBEVLQSNELDL-UHFFFAOYSA-N pyrrolidin-2-one Chemical compound O=C1CCCN1 HNJBEVLQSNELDL-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000003204 osmotic effect Effects 0.000 claims abstract description 6
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- 239000003795 chemical substances by application Substances 0.000 claims description 41
- 239000007787 solid Substances 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 210000002257 embryonic structure Anatomy 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 241000255588 Tephritidae Species 0.000 claims description 8
- 229920001223 polyethylene glycol Polymers 0.000 claims description 8
- 230000007423 decrease Effects 0.000 claims description 7
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- 229920001202 Inulin Polymers 0.000 claims description 6
- 241000244206 Nematoda Species 0.000 claims description 6
- 229920002125 Sokalan® Polymers 0.000 claims description 6
- 150000001241 acetals Chemical class 0.000 claims description 6
- 229920002678 cellulose Polymers 0.000 claims description 6
- 235000010980 cellulose Nutrition 0.000 claims description 6
- 229920003086 cellulose ether Polymers 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 6
- 229920001542 oligosaccharide Polymers 0.000 claims description 6
- 150000002482 oligosaccharides Chemical class 0.000 claims description 6
- 229920002401 polyacrylamide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 6
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims description 6
- 239000010453 quartz Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 229920000620 organic polymer Polymers 0.000 claims 6
- 239000000725 suspension Substances 0.000 claims 3
- 239000000523 sample Substances 0.000 description 37
- 230000003287 optical effect Effects 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 9
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- 238000012986 modification Methods 0.000 description 8
- 229920000642 polymer Polymers 0.000 description 7
- 238000005259 measurement Methods 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000010355 oscillation Effects 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 238000009652 hydrodynamic focusing Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
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- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000006196 drop Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 244000215068 Acacia senegal Species 0.000 description 1
- 235000006491 Acacia senegal Nutrition 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 229940012356 eye drops Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003676 hair preparation Substances 0.000 description 1
- 229920006158 high molecular weight polymer Polymers 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000015227 regulation of liquid surface tension Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 231100000735 select agent Toxicity 0.000 description 1
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- 150000003384 small molecules Chemical class 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N15/1409—Handling samples, e.g. injecting samples
- G01N2015/1411—Features of sheath fluids
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
L'invention concerne un cytomètre de flux destiné à l'analyse de particules de grande taille ou de petits organismes multicellulaires. Ce cytomètre risque d'être perturbé par un flux instable étant donné le diamètre important de la chambre d'écoulement. L'utilisation d'un fluide d'enveloppe ou d'une enveloppe et d'un échantillon, outre l'ajout d'un agent renforçateur de viscosité conférant une viscosité supérieure à celle de l'eau, constitue une garantie que le flux dans la section de pré-analyse sera un flux laminaire entièrement développé et que le flux dans la section d'analyse sera laminaire. Cela permet d'effectuer une analyse et un classement précis de particules de grande taille ou une analyse et un classement précis de particules plus petites à une vitesse accrue. L'utilisation de polymères solubles dans l'eau est recommandée du fait qu'ils augmentent la vitesse du fluide et ne produisent presque aucun effet osmotique. Une solution de 0,9 % en poids de pyrollidone polyvinylique présentant un poids moléculaire moyen de 1,3 million est particulièrement efficace. L'utilisation d'agents renforçateurs de viscosité entraînant une augmentation minimale de tension superficielle du fluide est également recommandée.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17357299P | 1999-12-29 | 1999-12-29 | |
| US60/173,572 | 1999-12-29 | ||
| PCT/US2000/035543 WO2001048455A2 (fr) | 1999-12-29 | 2000-12-29 | Reactif d'enveloppe a viscosite elevee pour cytometrie de flux |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2396015A1 true CA2396015A1 (fr) | 2001-07-05 |
Family
ID=22632632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002396015A Abandoned CA2396015A1 (fr) | 1999-12-29 | 2000-12-29 | Reactif d'enveloppe a viscosite elevee pour cytometrie de flux |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1242804A2 (fr) |
| JP (1) | JP2004500562A (fr) |
| AU (1) | AU2606301A (fr) |
| CA (1) | CA2396015A1 (fr) |
| WO (1) | WO2001048455A2 (fr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7116407B2 (en) | 1998-12-15 | 2006-10-03 | Union Biometrica, Inc. | System for axial pattern analysis of multicellular organisms |
| US7468789B2 (en) * | 2004-02-05 | 2008-12-23 | Advanced Analytical Technologies, Inc. | Flow cytometer for rapid bacteria detection |
| JP4606261B2 (ja) * | 2005-07-13 | 2011-01-05 | 三井造船株式会社 | 粒子測定方法 |
| US7867778B2 (en) * | 2007-02-23 | 2011-01-11 | Visiongate, Inc. | Fluid focusing for positional control of a specimen for 3-D imaging |
| CN103543093B (zh) * | 2007-06-07 | 2016-08-10 | 技术研究及发展基金有限公司 | 细胞测量设备 |
| JP2009115672A (ja) * | 2007-11-08 | 2009-05-28 | Sony Corp | 微小粒子の光学的測定方法及び分取方法、並びに前記光学的測定方法及び分取方法に用いる流路、光学的測定装置及びフローサイトメータ |
| JP4556996B2 (ja) | 2007-12-13 | 2010-10-06 | ソニー株式会社 | 光学的検出方法 |
| CN103608110B (zh) | 2011-03-09 | 2017-06-06 | 彼克斯赛尔医疗科技有限公司 | 用于制备用于分析的包含细胞的样品流体的一次性盒子 |
| KR102095617B1 (ko) * | 2013-03-15 | 2020-03-31 | 아이리스 인터내셔널 인크. | 혈액 샘플에서의 입자 분석을 위한 오토포커스 시스템 및 방법 |
| US9316635B2 (en) | 2013-03-15 | 2016-04-19 | Iris International, Inc. | Sheath fluid systems and methods for particle analysis in blood samples |
| US9857361B2 (en) | 2013-03-15 | 2018-01-02 | Iris International, Inc. | Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples |
| CN108780031A (zh) * | 2016-03-30 | 2018-11-09 | 西门子保健有限责任公司 | 使用环境粘弹性流体流对准样本流中的非球形生物实体 |
| JP6716057B2 (ja) * | 2016-04-08 | 2020-07-01 | 富山県 | 細胞を分離する方法及び装置 |
| WO2019057794A1 (fr) * | 2017-09-19 | 2019-03-28 | Hifibio Sas | Tri de particules dans un système microfluidique |
| WO2023006372A1 (fr) * | 2021-07-29 | 2023-02-02 | Technische Universität München | Détection d'objets biologiques moléculaires, d'objets biologiques cellulaires et d'agrégats cellulaires par microscopie quantitative à contraste de phase |
| EP4124846A1 (fr) * | 2021-07-29 | 2023-02-01 | Technische Universität München | Détection d'agrégats cellulaires en utilisant la microscopie quantitative à contraste de phase |
| CN116973279A (zh) * | 2022-04-22 | 2023-10-31 | 中国石油天然气股份有限公司 | 一种基于变粘度方式检测颗粒粒度的系统及方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69319715D1 (de) * | 1993-05-28 | 1998-08-20 | Enea Ente Nuove Tec | Verfahren zur Analyse, Abtrennung und Mikroverkapselung von zellulären Elementen |
| JPH09126989A (ja) * | 1995-11-07 | 1997-05-16 | Hitachi Ltd | フロー式粒子画像解析装置 |
| US6130309A (en) * | 1996-09-20 | 2000-10-10 | Tyndale Plains-Hunter, Ltd. | Hydrophilic polyether polyurethanes containing carboxylic acid |
-
2000
- 2000-12-29 JP JP2001548919A patent/JP2004500562A/ja not_active Withdrawn
- 2000-12-29 WO PCT/US2000/035543 patent/WO2001048455A2/fr not_active Ceased
- 2000-12-29 EP EP00989570A patent/EP1242804A2/fr not_active Withdrawn
- 2000-12-29 AU AU26063/01A patent/AU2606301A/en not_active Abandoned
- 2000-12-29 CA CA002396015A patent/CA2396015A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2606301A (en) | 2001-07-09 |
| WO2001048455A3 (fr) | 2002-05-10 |
| EP1242804A2 (fr) | 2002-09-25 |
| WO2001048455A2 (fr) | 2001-07-05 |
| JP2004500562A (ja) | 2004-01-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |