CA2420277A1 - Anticorps specifiques aux lysines methylees dans des histones - Google Patents

Anticorps specifiques aux lysines methylees dans des histones Download PDF

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Publication number
CA2420277A1
CA2420277A1 CA002420277A CA2420277A CA2420277A1 CA 2420277 A1 CA2420277 A1 CA 2420277A1 CA 002420277 A CA002420277 A CA 002420277A CA 2420277 A CA2420277 A CA 2420277A CA 2420277 A1 CA2420277 A1 CA 2420277A1
Authority
CA
Canada
Prior art keywords
antibody
seq
chromatin
histone
methyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002420277A
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English (en)
Inventor
C. David Allis
Brian D. Strahl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UVA Licensing and Ventures Group
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2420277A1 publication Critical patent/CA2420277A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La présente invention concerne la production d'anticorps d'histone spécifiques à la méthyllysine. Plus particulièrement, l'anticorps spécifique de méthylation de H3 lysine 4 (méthyl(K4)H3) se fixe sur l'histone H3 méthylée en lysine 4. La méthylation de la lysine 4 (K4) sur l'histone H3 a été associée à des régions transcriptionnellement actives de la chromatine. Un second anticorps, appelé anticorps spécifique de méthylation de H3 lysine 9 (méthyl(K9)H3), se fixe spécifiquement sur l'histone H3 méthylée en lysine 9. La méthylation de la lysine 9 (K9) sur l'histone H3 a été associée à l'extinction des gènes. Ces anticorps sont utiles pour identifier des régions de l'hétérochromatine et de l'euchromatine, et peuvent être destinés au diagnostic et à l'analyse.
CA002420277A 2000-08-25 2001-08-23 Anticorps specifiques aux lysines methylees dans des histones Abandoned CA2420277A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22776700P 2000-08-25 2000-08-25
US60/227,767 2000-08-25
US30274701P 2001-07-03 2001-07-03
US60/302,747 2001-07-03
PCT/US2001/026283 WO2002018418A1 (fr) 2000-08-25 2001-08-23 Anticorps specifiques aux lysines methylees dans des histones

Publications (1)

Publication Number Publication Date
CA2420277A1 true CA2420277A1 (fr) 2002-03-07

Family

ID=26921747

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002420277A Abandoned CA2420277A1 (fr) 2000-08-25 2001-08-23 Anticorps specifiques aux lysines methylees dans des histones

Country Status (5)

Country Link
EP (1) EP1313756A4 (fr)
JP (1) JP2004520270A (fr)
AU (1) AU2001285214A1 (fr)
CA (1) CA2420277A1 (fr)
WO (1) WO2002018418A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0111218D0 (en) 2001-05-08 2001-06-27 Cancer Res Ventures Ltd Assays methods and means
AU2003216291A1 (en) * 2002-02-20 2003-09-09 Chroma Therapeutics Ltd. A non-invasive diagnostic test utilizing histone modification markers
US7153691B2 (en) * 2002-11-13 2006-12-26 G6 Science Corp. Method of identifying and assessing DNA euchromatin in biological cells for detecting disease, monitoring wellness, assessing bio-activity, and screening pharmacological agents
WO2004080288A2 (fr) * 2003-03-10 2004-09-23 University Of Virginia Patent Foundation Modifications post-translationnelles de proteines en tant que commutations regulatrices
EP1707576A1 (fr) * 2003-12-02 2006-10-04 Advanced Life Science Institute, Inc. Anticorps reconnaissant la methyl-lysine, procede permettant de produire ces anticorps et utilisation de ces anticorps
EP1827454A4 (fr) * 2004-12-09 2008-07-16 Brigham & Womens Hospital Compositions et procedes a base de la kinase haspine
US20160069903A1 (en) 2014-09-10 2016-03-10 Fundació Institute De Ciències Foròniques Method for detecting cells
US10441644B2 (en) 2015-05-05 2019-10-15 The Regents Of The University Of California H3.3 CTL peptides and uses thereof
KR101590450B1 (ko) 2015-05-22 2016-02-02 중앙대학교 산학협력단 Filaggrin 유전자 프로모터의 히스톤 변형 검출 방법 및 이를 이용한 염증성 피부질환 진단용 조성물
CN108289937A (zh) 2015-06-03 2018-07-17 爱兰细胞技术公司 用于产生和递送来自干细胞的有益因子的方法和装置
CN109415431A (zh) * 2016-04-20 2019-03-01 爱兰细胞技术公司 与k180二甲基化h1.0蛋白相关的组合物和方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5989829A (en) * 1991-08-09 1999-11-23 Washington University Autoantibodies and their targets in the diagnosis of peripheral neuropathies
EP1411975A4 (fr) * 2001-07-03 2005-04-27 Univ Virginia Methylation de l'histone h4 au niveau de l'arginine 3

Also Published As

Publication number Publication date
EP1313756A4 (fr) 2004-12-22
AU2001285214A1 (en) 2002-03-13
JP2004520270A (ja) 2004-07-08
WO2002018418A1 (fr) 2002-03-07
EP1313756A1 (fr) 2003-05-28

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Legal Events

Date Code Title Description
FZDE Discontinued